Acute hepatotoxicity of a tetrachlorobiphenyl?Changes in the hepatocyte ultrastructure and plasma membrane-bound enzymes

1979 ◽  
Vol 8 (3) ◽  
pp. 321-333 ◽  
Author(s):  
F. S. Lin ◽  
M. T. Hsia ◽  
J. R. Allen
Keyword(s):  
Plasma Membrane ◽  
Hepatocyte Ultrastructure ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

scholarly journals ULTRASTRUCTURAL STUDY OF MEMBRANE-BOUND ENZYMES IN THYMOCYTES

1974 ◽  
Vol 22 (12) ◽  
pp. 1128-1134 ◽  
Author(s):  
ARIANE MONNERON ◽  
JEAN-CLAUDE BENICHOU ◽  
INSTITUT PASTEUR ◽  
YVETTE FLORENTIN ◽  
ELIANE GUERRY
Keyword(s):  
Plasma Membrane ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Uridine Diphosphate ◽  
Lead Phosphate ◽  
Isolated Nuclei ◽  
Whole Cells ◽  
Diphosphate Glucose ◽  
Membrane Bound ◽  
Membrane Bound Enzymes ◽  
Phosphate Deposits

Calf thymocytes in suspension, as well as isolated calf thymocyte nuclei, were incubated in the presence of several phosphorylated substrates. 5'-Nucleotidase was easily detected on the plasma membrane of thymocytes (external side), but could be demonstrated on isolated nuclei only to a small extent. No other substrates were detectably hydrolyzed by isolated nuclei except adenosine triphosphate and 3'-thymidine monophosphate. The surface of whole cells was found to be much more reactive. A 3'-nucleotidase activity was shown to occur on the plasma membrane of a number of thymocytes and produced large lead phosphate deposits, some of them protruding into the cytoplasm. Enzymic activities splitting β-nicotinamide adenine dinucleotide phosphate and uridine diphosphate glucose were also readily detectable on the surface of cells. Since the pattern of the lead phosphate deposits and the number of reactive cells varied with the added substrate, and since cells were compared with their isolated nuclei, the positive reactions were considered to indicate the presence (on the exposed membranes) of the corresponding enzymes on the exposed membranes.

Effects of ethanol administration on rat liver plasma membrane-bound enzymes

1985 ◽  
Vol 34 (15) ◽  
pp. 2685-2689 ◽  
Author(s):  
Jorge L. Gonzalez-Calvin ◽  
John B. Saunders ◽  
Ian R. Crossley ◽  
Christopher J. Dickenson ◽  
Heather M. Smith ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Ethanol Administration ◽  
Liver Plasma Membrane ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

Leukotriene D4 and E4 formation by plasma membrane bound enzymes

1984 ◽  
Vol 28 (6) ◽  
pp. 929-938 ◽  
Author(s):  
Christine G. Kuo ◽  
Matthew T. Lewis ◽  
Barbara A. Jakschik
Keyword(s):  
Plasma Membrane ◽  
Leukotriene D4 ◽  
Membrane Bound ◽  
Membrane Bound Enzymes

scholarly journals Alterations in the activities of hepatic plasma-membrane and microsomal enzymes during liver regeneration

1983 ◽  
Vol 212 (2) ◽  
pp. 445-452 ◽  
Author(s):  
G Deliconstantinos ◽  
G Ramantanis
Keyword(s):  
Plasma Membrane ◽  
Liver Regeneration ◽  
Membrane Fluidity ◽  
Plasma Membranes ◽  
Membrane Lipids ◽  
Biological Significance ◽  
Hill Coefficient ◽  
Membrane Bound ◽  
The Hill ◽  
Membrane Bound Enzymes

A marked increase in the activities of rat liver plasma-membrane (Na+ + K+)-stimulated ATPase and microsomal Ca2+-stimulated ATPase was observed 18h after partial hepatectomy. Lipid analyses for both membrane preparations reveal that in partially hepatectomized rats the cholesterol and sphingomyelin content are decreased with a subsequent decrease in the cholesterol/phospholipid molar ratio compared with those of sham-operated animals. Changes in the allosteric properties of plasma-membrane (Na+ + K+)-stimulated ATPase by F- (as reflected by changes in the Hill coefficient) indicated a fluidization of the lipid bilayer of both membrane preparations in 18 h-regenerating liver. The amphipathic dodecyl glucoside incorporated into the hepatic plasma membranes evoked a marked increase in the (Na+ + K+)-stimulated ATPase and 5′-nucleotidase activities. The lack of effect of the glucoside on the Lubrol-PX-solubilized 5′-nucleotidase indicates that changes in the activities of the membrane-bound enzymes caused by the glucoside are due to modulation of the membrane fluidity. Dodecyl glucoside appears to increase the membrane fluidity, evaluated through changes in the Hill coefficient for plasma-membrane (Na+ + K+)-stimulated ATPase. The biological significance of these data is discussed in terms of the differences and changes in the interaction of membrane-bound enzymes with membrane lipids during liver regeneration.

Effect of free fatty acids and cholesterol in vitro on liver plasma membrane-bound enzymes

1982 ◽  
Vol 38 (1) ◽  
pp. 102-104 ◽  
Author(s):  
S. Leoni ◽  
P. Luly ◽  
M. T. Mangiantini ◽  
S. Spagnuolo
Keyword(s):  
Fatty Acids ◽  
Plasma Membrane ◽  
Free Fatty Acids ◽  
Liver Plasma Membrane ◽  
Membrane Bound ◽  
Membrane Bound Enzymes
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