The effect of protein on preimplantation mouse embryo development in vitro

1984 ◽  
Vol 1 (3) ◽  
pp. 183-187 ◽  
Author(s):  
Catriona M. Caro ◽  
Alan Trounson
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Development In Vitro

Comparative effects of essential and nonessential metals on preimplantation mouse embryo development in vitro

Toxicology ◽  
1997 ◽  
Vol 116 (1-3) ◽  
pp. 123-131 ◽  
Author(s):  
Lynn A. Hanna ◽  
Jeffrey M. Peters ◽  
Lynn M. Wiley ◽  
Michael S. Clegg ◽  
Carl L. Keen
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Development In Vitro

Effects of sera from patients given various anesthetics on preimplantation mouse embryo development in vitro

1991 ◽  
Vol 8 (4) ◽  
pp. 191-197 ◽  
Author(s):  
Dennis W. Matt ◽  
Kenneth A. Steingold ◽  
Celia M. Dastvan ◽  
Cathy A. James ◽  
Walter Dunwiddie
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Development In Vitro

scholarly journals O-085. Effect of EGF on preimplantation mouse embryo development in vitro

1997 ◽  
Vol 12 (Suppl_2) ◽  
pp. 40-40
Author(s):  
M. Argirou ◽  
R. Bletsa ◽  
D. Loutradis ◽  
S. Davies ◽  
T. Patarias ◽  
...  
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Development In Vitro

scholarly journals Effects of peritoneal fluid on preimplantation mouse embryo development and apoptosis in vitro

2005 ◽  
Vol 11 (5) ◽  
pp. 615-619 ◽  
Author(s):  
Navid Esfandiari ◽  
Tommaso Falcone ◽  
Jeffrey M Goldberg ◽  
Ashok Agarwal ◽  
Rakesh K Sharma
Keyword(s):  
Embryo Development ◽  
Mouse Embryo ◽  
Peritoneal Fluid ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse

scholarly journals 111. THE ROLE OF L-PROLINE IN PREIMPLANTATION MOUSE EMBRYO DEVELOPMENT IN VITRO

2010 ◽  
Vol 22 (9) ◽  
pp. 29 ◽  
Author(s):  
S. Ozsoy ◽  
M. B. Morris ◽  
M. L. Day
Keyword(s):  
Amino Acids ◽  
Embryo Development ◽  
Preimplantation Embryo ◽  
Blastocyst Stage ◽  
Low Density ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Development In Vitro

Amino acids are known to play important roles in preimplantation embryo development, including regulation of cell volume and metabolism. Inclusion of l-glutamine, glycine and betaine in embryo culture medium has been shown to improve development in vitro by acting as organic osmolytes, thereby regulating cell volume. The purpose of the present study was to examine the effect of l-proline on preimplantation mouse embryo development in vitro. One-cell stage embryos were cultured in modified HTF, at low density (1 embryo/100 μL) and high density (1 embryo/μL) in the presence and absence of amino acids. Development of the embryos was scored every 24 h until the blastocyst stage. At low density, l-proline significantly increased the proportion of embryos developing to the blastocyst stage. This effect was abolished by culture at high density, suggesting that l-proline was activating a pathway similar to that involved in autocrine signalling by trophic factors in the preimplantation embryo. The improvement in development observed in the presence of l-proline was not due to its action as an organic osmolyte since the osmolality of the medium was 270 mOsm. Furthermore, glycine and betaine, which are known to act as osmolytes in embryos, had no effect on blastocyst development. In embryonic stem cells L-proline is taken up by an amino acid transporter and is involved in the regulation of growth and differentiation (1). The present data suggest that l-proline may have a similar, important role in preimplantation development. (1) JM Washington, J Rathjen, F Felquer, A Lonic, MD Bettess, N Hamra, L Semendric, BSN Tan, J-A Lake, RA Keough, MB Morris and PD Rathjen (2010) Am J Physiol Cell Physiol 298: C982–C992.

Effect of zinc and copper on preimplantation mouse embryo development in vitro and metallothionein levels

Zygote ◽  
1993 ◽  
Vol 1 (3) ◽  
pp. 225-229 ◽  
Author(s):  
Francesca Vidal ◽  
Juan Hidalgo
Keyword(s):  
Embryo Development ◽  
Developmental Stages ◽  
Blastocyst Stage ◽  
Preimplantation Embryos ◽  
Mrna Levels ◽  
Mouse Embryo Development ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse ◽  
Development In Vitro

The effect of zinc and copper on the in vitro development of mouse preimplantation embryos and on metallothionein (MT) levels was studied by exposing the embryos to 100 μM concentrations of the metals for 24 h at the 1-cell,2-cell, 6-8-cell, morula and blastocyst stages. Zinc affected embryo development in the early but not in the late stages, whereas copper affected it more generally. The combined presence of both metals caused a stronger embryotoxicity. MT levels were measured by radioimmunoassay and were found to be similar at all developmental stages, though possibly higher at the blastocyst stage. The exposure of embryos to zinc and copper increased MT levels significantly only at the blastocyst stage, supporting previously published results on MT mRNA levels.

Sign in / Sign up

Export Citation Format

Share Document