Ionic channels, ion transport and plant cell membranes: Potential applications of the patch-clamp technique

PROTOPLASMA ◽  
1985 ◽  
Vol 127 (3) ◽  
pp. 147-162 ◽  
Author(s):  
K. Takeda ◽  
A. C. Kurkdjian ◽  
R. T. Kado
Keyword(s):  
Patch Clamp ◽  
Ion Transport ◽  
Plant Cell ◽  
Cell Membranes ◽  
Ionic Channels ◽  
Patch Clamp Technique ◽  
Clamp Technique ◽  
Potential Applications ◽  
Plant Cell Membranes

Patch-clamp technique in renal physiology

1986 ◽  
Vol 250 (3) ◽  
pp. F379-F385 ◽  
Author(s):  
L. G. Palmer
Keyword(s):  
Ion Channels ◽  
Patch Clamp ◽  
Ion Transport ◽  
Renal Physiology ◽  
Patch Clamp Technique ◽  
Clamp Technique ◽  
Basic Concepts

The patch-clamp technique of Neher and Sakmann and their colleagues has been widely used over the last 5 years to investigate ion channels in excitable tissues. More recently, it has become useful as a tool to study channels involved in transepithelial ion transport. In this review, I briefly cover the basic concepts behind the patch-clamp technique and the kinds of information that can be obtained with it. I then summarize the applications of the technique to renal tissues and describe some of the channel types that have been observed to date in epithelia.

Ionic channels in plant cell membranes

1988 ◽  
Vol 72 (4) ◽  
pp. 816-820 ◽  
Author(s):  
Ruth L. Satter ◽  
Nava Moran
Keyword(s):  
Plant Cell ◽  
Cell Membranes ◽  
Ionic Channels ◽  
Plant Cell Membranes

Ketamine blocks Ca2+-activated K+ channels in rabbit cerebral arterial smooth muscle cells

2003 ◽  
Vol 285 (3) ◽  
pp. H1347-H1355 ◽  
Author(s):  
Jin Han ◽  
Nari Kim ◽  
Hyun Joo ◽  
Euiyong Kim
Keyword(s):  
Smooth Muscle ◽  
Patch Clamp ◽  
Smooth Muscle Cells ◽  
Cerebral Arteries ◽  
Muscle Cells ◽  
Channel Activity ◽  
Patch Clamp Technique ◽  
Arterial Smooth Muscle ◽  
Clamp Technique ◽  
Arterial Smooth Muscle Cells

Although ketamine and Ca2+-activated K+ (KCa) channels have been implicated in the contractile activity regulation of cerebral arteries, no studies have addressed the specific interactions between ketamine and the KCa channels in cerebral arteries. The purpose of this study was to examine the direct effects of ketamine on KCa channel activities using the patch-clamp technique in single-cell preparations of rabbit middle cerebral arterial smooth muscle. We tested the hypothesis that ketamine modulates the KCa channel activity of the cerebral arterial smooth muscle cells of the rabbit. Vascular myocytes were isolated from rabbit middle cerebral arteries using enzymatic dissociation. Single KCa channel activities of smooth muscle cells from rabbit cerebral arteries were recorded using the patch-clamp technique. In the inside-out patches, ketamine in the micromolar range inhibited channel activity with a half-maximal inhibition of the ketamine conentration value of 83.8 ± 12.9 μM. The Hill coefficient was 1.2 ± 0.3. The slope conductance of the current-voltage relationship was 320.1 ± 2.0 pS between 0 and +60 mV in the presence of ketamine and symmetrical 145 mM K+. Ketamine had little effect on either the voltage-dependency or open- and closed-time histograms of KCa channel. The present study clearly demonstrates that ketamine inhibits KCa channel activities in rabbit middle cerebral arterial smooth muscle cells. This inhibition of KCa channels may represent a mechanism for ketamine-induced cerebral vasoconstriction.

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