Rapid purification of native SecA fromEscherichia coli: Development of a new affinity chromatography procedure

1994 ◽  
Vol 29 (6) ◽  
pp. 323-329 ◽  
Author(s):  
Kevin B. Kiser ◽  
Philippe Arnaud ◽  
Michael G. Schmidt
Keyword(s):  
Affinity Chromatography ◽  
Fromescherichia Coli ◽  
Rapid Purification

Yeast Aminopeptidase II: Rapid Purification Using Affinity Chromatography of the Periplasmic Enzyme

1982 ◽  
Vol 37 (9) ◽  
pp. 845-848 ◽  
Author(s):  
Joseph Knüver ◽  
Klaus-Heinrich Röhm
Keyword(s):  
Affinity Chromatography ◽  
Ammonium Sulfate ◽  
Isolation Method ◽  
Yeast Protein ◽  
Rapid Purification ◽  
Periplasmic Enzyme

Periplasmic aminopeptidase II of yeast was isolated from protoplast supernatants by ammonium sulfate precipita­tion, DEAE-Sephacel chromatography and affinity chromatography on immobilized bestatin. This isolation method is more rapid than conventional procedures and, in addition, avoids contact of the enzyme with yeast protein-ases. Thus the preparation obtained is likely to represent the native periplasmic from of aminopeptidase II.

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