Rapid purification of the 30 kDa calcimedin using DNase I affinity chromatography

1989 ◽  
Vol 159 (2) ◽  
pp. 828-833 ◽  
Author(s):  
Vana L. Smith ◽  
John R. Dedman
Keyword(s):  
Affinity Chromatography ◽  
Dnase I ◽  
Rapid Purification

Yeast Aminopeptidase II: Rapid Purification Using Affinity Chromatography of the Periplasmic Enzyme

1982 ◽  
Vol 37 (9) ◽  
pp. 845-848 ◽  
Author(s):  
Joseph Knüver ◽  
Klaus-Heinrich Röhm
Keyword(s):  
Affinity Chromatography ◽  
Ammonium Sulfate ◽  
Isolation Method ◽  
Yeast Protein ◽  
Rapid Purification ◽  
Periplasmic Enzyme

Periplasmic aminopeptidase II of yeast was isolated from protoplast supernatants by ammonium sulfate precipita­tion, DEAE-Sephacel chromatography and affinity chromatography on immobilized bestatin. This isolation method is more rapid than conventional procedures and, in addition, avoids contact of the enzyme with yeast protein-ases. Thus the preparation obtained is likely to represent the native periplasmic from of aminopeptidase II.

Rapid Purification of Human DNase I Using Mouse Monoclonal Anti-DNase I Antibodies and Characterization of the Antibodies

2000 ◽  
Vol 17 (2) ◽  
pp. 71-76 ◽  
Author(s):  
Tamiko Nakajima ◽  
Toshihiro Yasuda ◽  
Haruo Takeshita ◽  
Yoshimitsu Nakashima ◽  
Shinjiro Mori ◽  
...  
Keyword(s):  
Dnase I ◽  
Rapid Purification
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