scholarly journals Rapid purification of phospholipase A2 from Crotalus adamanteus venom by affinity chromatography

1975 ◽  
Vol 250 (16) ◽  
pp. 6564-6566
Author(s):  
CO Rock ◽  
F Snyder
Keyword(s):  
Phospholipase A2 ◽  
Affinity Chromatography ◽  
Rapid Purification

Yeast Aminopeptidase II: Rapid Purification Using Affinity Chromatography of the Periplasmic Enzyme

1982 ◽  
Vol 37 (9) ◽  
pp. 845-848 ◽  
Author(s):  
Joseph Knüver ◽  
Klaus-Heinrich Röhm
Keyword(s):  
Affinity Chromatography ◽  
Ammonium Sulfate ◽  
Isolation Method ◽  
Yeast Protein ◽  
Rapid Purification ◽  
Periplasmic Enzyme

Periplasmic aminopeptidase II of yeast was isolated from protoplast supernatants by ammonium sulfate precipita­tion, DEAE-Sephacel chromatography and affinity chromatography on immobilized bestatin. This isolation method is more rapid than conventional procedures and, in addition, avoids contact of the enzyme with yeast protein-ases. Thus the preparation obtained is likely to represent the native periplasmic from of aminopeptidase II.

Use of a phosphopeptide as a ligand to purify phospholipase A2 from the venom of Crotalus durisuss terrificus by affinity chromatography

2020 ◽  
Vol 1146 ◽  
pp. 122070 ◽  
Author(s):  
Soledad L. Saavedra ◽  
Gerardo Acosta ◽  
Lucía Ávila ◽  
Silvana L. Giudicessi ◽  
Silvia A. Camperi ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Affinity Chromatography
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