Analysis of electrophoretic whole-cell protein profiles as a tool for characterization ofEnterococcus species

1994 ◽  
Vol 28 (3) ◽  
pp. 149-153 ◽  
Author(s):  
Vania Lucia Carreira Merquior ◽  
Jose Mauro Peralta ◽  
Richard R. Facklam ◽  
Lucia Martins Teixeira
Keyword(s):  
Cell Protein ◽  
Protein Profiles ◽  
Whole Cell ◽  
Whole Cell Protein

scholarly journals  Analysis of whole cell protein profiles of Salmonella serovars isolated from chicken, turkey and sheep faeces by SDS-PAGE

2010 ◽  
Vol 55 (No. 6) ◽  
pp. 259-263 ◽  
Author(s):  
A. Aksakal
Keyword(s):  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Visual Assessment ◽  
Cell Protein ◽  
Protein Profiles ◽  
Whole Cell ◽  
Sds Page ◽  
Molecular Masses ◽  
Whole Cell Protein ◽  
Salmonella Serovars

This study was carried out to determine the whole cell protein profiles of Salmonella serovars from chicken, turkey and sheep faeces by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A total of 34 Salmonella strains were included in the study, 14 of them were isolated from chicken, 14 from turkey and six from sheep. SDS-PAGE was carried out using 12% (w/v) separating and 4% (w/v) stacking gels. The results showed more than 30 protein bands ranging in size from 97 kDa (kilodaltons) to below 14.4 kDa as determined by visual assessment of their approximate molecular masses. Protein bands of 78.1, 51.2, 41.5, 37.3, 35.1, 33.9, 30.7, 27.6, 25.4, and 24 kDa were detected in all Salmonella serovars. Salmonella strains used in this study were closely related and could not be differentiated depending on the whole cell protein profiles using SDS-PAGE.

scholarly journals Whole-Cell Protein Profiles of Disintegrated Freshwater Green Algae and Cyanobacterium

2014 ◽  
Vol 25 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Dušan Samek ◽  
Ladislava Mišurcová ◽  
Ludmila Machů ◽  
Leona Buňková ◽  
Antonín Minařík ◽  
...  
Keyword(s):  
Green Algae ◽  
Cell Protein ◽  
Protein Profiles ◽  
Whole Cell ◽  
Whole Cell Protein

scholarly journals Differentiation of Pathogenic Groups of Xylella fastidiosa Strains with Whole-Cell Protein Profiles

Plant Disease ◽  
2002 ◽  
Vol 86 (8) ◽  
pp. 875-879 ◽  
Author(s):  
R. L. Wichman ◽  
D. L. Hopkins
Keyword(s):  
Xylella Fastidiosa ◽  
Sodium Dodecyl ◽  
Mass Range ◽  
Cell Protein ◽  
Pierce's Disease ◽  
Protein Profiles ◽  
Whole Cell ◽  
Leaf Scorch ◽  
Pierce’S Disease ◽  
Whole Cell Protein

Whole-cell protein analyses of 75 Xylella fastidiosa strains by sodium dodecyl sulfatepolyacrylamide gel electrophoresis were compared, and variations in the protein banding patterns among the strains were observed. Based on the presence, absence, or difference in intensity of 10 protein bands within the 21.5 to 45.0 kDa molecular mass range, the strains could be subdivided into four distinct pathogenic groups and two miscellaneous groups whose members were pathogenic to various different hosts. Group 1 was the Pierce's disease of grapevine pathogenic group. Although 4 of these 45 strains had hosts of origin other than grapevine, they all produced Pierce's disease symptoms. Uniform, distinct protein profiles also occurred with group 2 (elderberry leaf scorch strains), group 3 (oak leaf scorch strains), and group 4 (oleander leaf scorch strains). Groups 5 and 6 were made up of strains pathogenic to almond, blackberry, lupine, mulberry, periwinkle, elm, and plum. Thus, whole-cell protein analysis was shown to be a rapid and consistent method for identifying four pathogenic groups of X. fastidiosa strains.

An extended QBASIC program for the normalization and computation of whole-cell protein profiles and the application to clinically importantCandidaspecies

1990 ◽  
Vol 28 (4) ◽  
pp. 303-315 ◽  
Author(s):  
E.J. Smit ◽  
P.W.J. Van Wyk ◽  
J.P.J. v.d. Westhuizen ◽  
J.L.F. Kock
Keyword(s):  
Cell Protein ◽  
Protein Profiles ◽  
Whole Cell ◽  
Whole Cell Protein

scholarly journals Diversity and differentiation among fluorescent pseudomonads in crop rhizospheres with whole-cell protein profiles

2008 ◽  
Vol 163 (5) ◽  
pp. 571-578 ◽  
Author(s):  
V. Shanmugam ◽  
Naosekpam Singh Ajit ◽  
Rajni Verma ◽  
Vivek Sharma
Keyword(s):  
Fluorescent Pseudomonads ◽  
Cell Protein ◽  
Protein Profiles ◽  
Whole Cell ◽  
Whole Cell Protein

scholarly journals Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines

2006 ◽  
Vol 56 (5) ◽  
pp. 973-978 ◽  
Author(s):  
Yoshinori Sato ◽  
Hirofumi Nishihara ◽  
Masao Yoshida ◽  
Makiko Watanabe ◽  
Jose D. Rondal ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Sequence Similarity ◽  
The Philippines ◽  
Cell Protein ◽  
Rrna Gene ◽  
Protein Profiles ◽  
Whole Cell ◽  
Chemolithotrophic Bacteria ◽  
Whole Cell Protein

Taxonomic studies were performed on ten hydrogen-oxidizing, facultatively chemolithotrophic bacteria that were isolated from volcanic mudflow deposits derived from the eruption of Mt. Pinatubo in the Philippines in 1991. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the genus Cupriavidus of the Betaproteobacteria; sequence similarity values with their nearest phylogenetic neighbour, Cupriavidus basilensis, were 97.1–98.3 %. In addition to phylogenetic analysis, results of whole-cell protein profiles and biochemical tests revealed that these strains were members of two distinct species. DNA–DNA hybridizations and whole-cell protein profiles enabled these isolates to be differentiated from related Cupriavidus species with validly published names. The isolates were aerobic, Gram-negative, non-sporulating, peritrichously flagellated rods. Their G+C contents ranged from 65.2 to 65.9 mol% and their major isoprenoid quinone was ubiquinone Q-8. On the basis of these results, two novel species are proposed, Cupriavidus pinatubonensis sp. nov. [nine strains, with 1245T (=CIP 108725T=PNCM 10346T) as the type strain] and Cupriavidus laharis sp. nov. [one strain, the type strain 1263aT (=CIP 108726T=PNCM 10347T)]. It is also suggested that Ralstonia sp. LMG 1197 (=JMP 134) should be included in the species C. pinatubonensis.

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