Quantitative histochemical determination of succinic dehydrogenase activity in skeletal muscle fibres

1988 ◽  
Vol 20 (4) ◽  
pp. 230-243 ◽  
Author(s):  
C. E. Blanco ◽  
G. C. Sieck ◽  
V. R. Edgerton
Keyword(s):  
Skeletal Muscle ◽  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Muscle Fibres ◽  
Succinic Dehydrogenase Activity ◽  
Skeletal Muscle Fibres

scholarly journals AN OSMIOPHILIC DISTYRYL DITETRAZOLIUM SALT (DS-NBT) FOR ULTRASTRUCTURAL DEHYDROGENASE ACTIVITY

1971 ◽  
Vol 19 (5) ◽  
pp. 273-285 ◽  
Author(s):  
ARNOLD M. SELIGMAN ◽  
IZHAK NIR ◽  
ROBERT E. PLAPINGER
Keyword(s):  
Skeletal Muscle ◽  
Dehydrogenase Activity ◽  
Succinic Dehydrogenase ◽  
Lactic Dehydrogenase ◽  
Structural Features ◽  
Rat Skeletal Muscle ◽  
Succinic Dehydrogenase Activity ◽  
Tissue Penetration ◽  
Diaphorase Activity ◽  
Doubly Charged

The preparation of a new osmiophilic ditetrazolium salt (DS-NBT) containing two styryl groups, but retaining the essential structural features of nitro-BT, is given. DS-NBT may be used to demonstrate succinic dehydrogenase activity (SDH) faster than with nitro-BT and fully as rapidly as with TC-NBT. DS-NBT is more stable than TC-NBT and it is more reliably and more readily osmicated than TC-NBT. The ultrastructural sites of SDH and NADH2 diaphorase activity are similar to those shown for cytochrome oxidase activity with diaminobenzidine in rat heart. The sites of lactic dehydrogenase activity are found in the sarcoplasmic reticulum and mitochondria of rat skeletal muscle fixed in formaldehyde for 3 hr. Tissue penetration of the doubly charged ditetrazolium salt (DS-NBT) was facilitated by 40-sec sonication and by 5% dimethyl sulfoxide. Illustrative examples and controls are included.

The cytochemical determination of enzyme activities in single skeletal-muscle fibres from patients with a partial deficiency of cytochrome oxidase

1985 ◽  
Vol 13 (4) ◽  
pp. 730-730 ◽  
Author(s):  
HEINZ REICHMANN ◽  
MARGARET A. JOHNSON ◽  
DOUGLASS M. TURNBULL ◽  
H. STANLEY A. SHERRATT
Keyword(s):  
Skeletal Muscle ◽  
Cytochrome Oxidase ◽  
Enzyme Activities ◽  
Muscle Fibres ◽  
Partial Deficiency ◽  
Skeletal Muscle Fibres

Succinic Dehydrogenase Activity of the Heart and Skeletal Muscle of Exercised Rats

1956 ◽  
Vol 185 (2) ◽  
pp. 348-350 ◽  
Author(s):  
George R. Hearn ◽  
W. W. Wainio
Keyword(s):  
Skeletal Muscle ◽  
Body Weight ◽  
Dehydrogenase Activity ◽  
Skeletal Muscles ◽  
Succinic Dehydrogenase ◽  
Albino Rats ◽  
Heart Ventricle ◽  
Succinic Dehydrogenase Activity ◽  
Heart Ventricles ◽  
Wistar Institute

Male albino rats of the Wistar Institute strain with an average beginning weight of 250 gm were exercised from 5–8 weeks on a training regimen consisting of swimming for 1/2 hour daily. Pair-fed, nonswimming animals served as controls. All exercised animals gained less body weight than did their controls. The adrenals and heart ventricles of the exercised animals hypertrophied significantly ( P < 0.01), while the skeletal muscles (gastrocnemii) did not. Succinic dehydrogenase activities (reduction of ferricytochrome c) were determined in the heart ventricles and the gastrocnemii. The results were expressed in terms of unit activities, actual total activities and relative total activities. The exercise, whether for 5, 6, 7 or 8 weeks, did not significantly alter the unit and actual total activities of either tissue, or the relative total activities of skeletal muscles, but did significantly increase the relative total activities of the heart ventricle ( P < 0.02).

scholarly journals DETERMINATION OF SUCCINIC DEHYDROGENASE ACTIVITY IN TISSUE CULTURE

1959 ◽  
Vol 7 (2) ◽  
pp. 144-146 ◽  
Author(s):  
NATHAN KAUFMAN ◽  
ROBERT HILL
Keyword(s):  
Tissue Culture ◽  
Dehydrogenase Activity ◽  
Hela Cells ◽  
Succinic Dehydrogenase ◽  
Protective Layer ◽  
The Other ◽  
Tissue Cultures ◽  
Succinic Dehydrogenase Activity

Two methods of demonstrating succinic dehydrogenase activity in tissue cultures of HeLa cells have been demonstrated. One method employs a protective layer of agar applied to the cells prior to incubation in the selected substrate. The other method uses a mixture of agar and substrate which is placed on the cells; incubation follows without the further addition of substrate.

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