Muscarinic receptor subtypes in equine tracheal smooth muscle

1992 ◽  
Vol 16 (4) ◽  
pp. 301-310 ◽  
Author(s):  
M. Yu ◽  
N. E. Robinson ◽  
Z. Wang ◽  
F. J. Derksen
Keyword(s):  
Smooth Muscle ◽  
Muscarinic Receptor ◽  
Tracheal Smooth Muscle ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes

Muscarinic receptor subtypes in canine tracheal smooth muscle

1995 ◽  
Vol 50 (3) ◽  
pp. 374
Author(s):  
Manabu Kagaya ◽  
Takanobu Shioya ◽  
Shun Kondo ◽  
Miki Itaba ◽  
Masaaki Sano ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscarinic Receptor ◽  
Tracheal Smooth Muscle ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes

Human esophageal smooth muscle cells express muscarinic receptor subtypes M1 through M5

2000 ◽  
Vol 279 (5) ◽  
pp. G1059-G1069 ◽  
Author(s):  
Jian Wang ◽  
Pawel S. Krysiak ◽  
Lisanne G. Laurier ◽  
Stephen M. Sims ◽  
Harold G. Preiksaitis
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Muscarinic Receptor ◽  
Muscle Cells ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
Functional Responses ◽  
Selective Antagonist ◽  
Esophageal Physiology ◽  
Esophageal Smooth Muscle Cells

Receptor characterization in human esophageal smooth muscle is limited by tissue availability. We used human esophageal smooth muscle cells in culture to examine the expression and function of muscarinic receptors. Primary cultures were established using cells isolated by enzymatic digestion of longitudinal muscle (LM) and circular muscle (CM) obtained from patients undergoing esophagectomy for cancer. Cultured cells grew to confluence after 10–14 days in medium containing 10% fetal bovine serum and stained positively for anti-smooth muscle specific α-actin. mRNA encoding muscarinic receptor subtypes M1–M5 was identified by RT-PCR. The expression of corresponding protein for all five subtypes was confirmed by immunoblotting and immunocytochemistry. Functional responses were assessed by measuring free intracellular Ca2+ concentration ([Ca2+]i) using fura 2 fluorescence. Basal [Ca2+]i, which was 135 ± 22 nM, increased transiently to 543 ± 29 nM in response to 10 μM ACh in CM cells ( n = 8). This response was decreased <95% by 0.01 μM 4-diphenylacetoxy- N-methylpiperidine, a M1/M3-selective antagonist, whereas 0.1 μM methoctramine, a M2/M4-selective antagonist, and 0.1 μM pirenzepine, a M1-selective antagonist, had more modest effects. LM and CM cells showed similar results. We conclude that human smooth muscle cells in primary culture express five muscarinic receptor subtypes and respond to ACh with a rise in [Ca2+]i mediated primarily by the M3 receptor and involving release of Ca2+ from intracellular stores. This culture model provides a useful tool for further study of esophageal physiology.

Muscarinic receptor subtypes in human and rat colon smooth muscle

1992 ◽  
Vol 43 (11) ◽  
pp. 2413-2419 ◽  
Author(s):  
Aurelio Gómez ◽  
Francisco Martos ◽  
Inmaculada Bellido ◽  
Elisabel Marquez ◽  
Antonio J. Garcia ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscarinic Receptor ◽  
Rat Colon ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes

Muscarinic receptor subtypes involved in carbachol-induced contraction of mouse uterine smooth muscle

2007 ◽  
Vol 377 (4-6) ◽  
pp. 503-513 ◽  
Author(s):  
Takio Kitazawa ◽  
Ryuichi Hirama ◽  
Kozue Masunaga ◽  
Tatsuro Nakamura ◽  
Koichi Asakawa ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Muscarinic Receptor ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
Uterine Smooth Muscle

The Role of M2 Muscarinic Receptor Subtypes Mediating Contraction of the Circular and Longitudinal Smooth Muscle of the Pig Proximal Urethra

2002 ◽  
Vol 168 (1) ◽  
pp. 308-314 ◽  
Author(s):  
Tomonori Yamanishi ◽  
Christopher R. Chapple ◽  
Kosaku Yasuda ◽  
Ken-Ichiro Yoshida ◽  
Russell Chess-Williams
Keyword(s):  
Smooth Muscle ◽  
Muscarinic Receptor ◽  
Receptor Subtypes ◽  
Muscarinic Receptor Subtypes ◽  
Longitudinal Smooth Muscle ◽  
M2 Muscarinic Receptor ◽  
Proximal Urethra
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