Osmiophilic cytoplasmic inclusions in the lymph node post-capillary venules of the guinea pig

1981 ◽  
Vol 178 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Chr Kittas ◽  
L. Henry ◽  
C. Papadimitriou ◽  
N. X. Papacharalampous
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Cytoplasmic Inclusions

Interlaboratory Evaluation of the Local Lymph Node Assay with 25 Chemicals and Comparison with Guinea Pig Test Data

1991 ◽  
Vol 1 (1) ◽  
pp. 30-43 ◽  
Author(s):  
D. A. Basketter ◽  
E. W. Scholes ◽  
I. Kimber ◽  
P. A. Botham ◽  
J. Hilton ◽  
...  
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Test Data ◽  
Local Lymph Node Assay ◽  
Local Lymph Node

scholarly journals Transfer of experimental allergic encephalomyelitis in Lewis rats using supernates of incubated sensitized lymph node cells.

1977 ◽  
Vol 145 (5) ◽  
pp. 1405-1410 ◽  
Author(s):  
C C Whitacre ◽  
P Y Paterson
Keyword(s):  
Spinal Cord ◽  
Lymph Node ◽  
Guinea Pig ◽  
Experimental Allergic Encephalomyelitis ◽  
Nervous Tissue ◽  
Allergic Encephalomyelitis ◽  
Lewis Rats ◽  
Transfer Activity ◽  
Lymph Node Cells ◽  
Experimental Allergic

Supernates derived from incubated lymph node cells of Lewis rats sensitized to guinea pig spinal cord-Freund's adjuvant transfer experimental allergic encephalomyelitis (EAE) to syngeneic recipients. EAE supernatant transfer activity (EAE-STA) is not demonstrable in supernates derived from LNC of control donors not sensitized to nervous tissue. After addition of brain antigen to active supernates, EAE-STA is not longer demonstrable.

Fibronectin Distribution during Lymph Node Development in Guinea Pig: An Immunohistochemical Study

1986 ◽  
Vol 126 (3) ◽  
pp. 160-162 ◽  
Author(s):  
A. De Pasquale ◽  
G. Biagini ◽  
S. Pileri ◽  
M. Franchini ◽  
V. Bottanelli ◽  
...  
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Immunohistochemical Study ◽  
Node Development

Cytotoxic activity of guinea-pig lymph-node cells stimulatedin vitro

1978 ◽  
Vol 22 (5) ◽  
pp. 595-601 ◽  
Author(s):  
Amnon Altman ◽  
Herbert J. Rapp
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Cytotoxic Activity ◽  
Lymph Node Cells

scholarly journals OCCURRENCE OF DELAYED HYPERSENSITIVITY DURING THE DEVELOPMENT OF ARTHUS TYPE HYPERSENSITIVITY

1958 ◽  
Vol 107 (1) ◽  
pp. 109-124 ◽  
Author(s):  
S. B. Salvin ◽  
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Latent Period ◽  
Guinea Pigs ◽  
Specific Antigen ◽  
Egg Albumin ◽  
Lymph Node Cells ◽  
Type Inclusion ◽  
Antibody Determination ◽  
Circulating Antibody

Guinea pigs were injected in the footpads with either purified diphtheria toxoid or recrystallized egg albumin in Freund adjuvant without mycobacteria. Each guinea pig was then skin-tested only once with the specific antigen and bled for antibody determination. After injection of the sensitizing antigen, a latent period occurred during which neither sensitivity nor circulating antibody could be detected. A period of delayed sensitivity followed wherein circulating antibody could not be discerned and which could be transferred by lymph node cells. Ultimately, the Arthus type sensitivity developed, accompanied by circulating antibody. The duration and severity of reactions to homologous antigens during the last 2 phases varied with the antigen and with the dose. An increase in the sensitizing dose decreased the duration of the delayed type of allergy, a decrease in the dose prolonged the delayed type. Inclusion of mycobacterium in the sensitizing inoculum tended to introduce delayed sensitivity earlier and delay the onset of Arthus type sensitivity. When specific precipitate in antibody excess was included with the toxoid in the sensitizing dose, the onset of the Arthus phase was hastened. When lymph nodes from a large number of sensitized donors were removed during the latter part of the latent period, recipients of the cells showed a delayed type sensitivity.

Screening Petrochemicals for Contact Hypersensitivity Potential: A Comparison of the Murine Local Lymph Node Assay with Guinea Pig and Human Test Data

1994 ◽  
Vol 23 (2) ◽  
pp. 179-187
Author(s):  
D. A. EDWARDS ◽  
T. M. SORANNO ◽  
M. A. AMORUSO ◽  
R. V. HOUSE ◽  
A. C. TUMMEY ◽  
...  
Keyword(s):  
Lymph Node ◽  
Guinea Pig ◽  
Test Data ◽  
Contact Hypersensitivity ◽  
Local Lymph Node Assay ◽  
Local Lymph Node

scholarly journals Inhibition of T-antigen-binding cells by idiotypic antisera.

1977 ◽  
Vol 146 (3) ◽  
pp. 766-778 ◽  
Author(s):  
C A Prange ◽  
J Fiedler ◽  
D E Nitecki ◽  
C J Bellone
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Guinea Pig ◽  
T Cell Receptors ◽  
Binding Assay ◽  
T Antigen ◽  
Antigen Binding ◽  
Variable Regions ◽  
Heterologous System ◽  
Lymph Node Cells

Shared idiotypy between B- and T-cell receptors specific for the antigen L-tyrosine-p-azophenyltrimethylammonium [tyr(TMA)] was studied in an antigen-binding assay using idiotypic antisera. These idiotypic reagents were prepared by inoculation of rabbits with purified anti-tyr(TMA) antibody raised in strain 13 guinea pigs. The antisera blocked 78-83% of the antigen-binding T cells (T-ABC) and 50-55% of the antigen-binding B cells (B-ABC) from tyr(TMA)-immune strain 13 and outbred lymph node cells (LNC). An excess of normal guinea pig Ig in the ABC assay did not affect the ability of the idiotypic antisera to block T- and B-ABC. Nylon wool-passed tyr(TMA)-immune LNC were trypsin treated resulting in a 75% loss of T-ABC. The trypsin-treated population was then cultured for 16 h which resulted in a return of T-ABC to 92% of pretrypsin values. 77% of these regenerated T-ABC could be blocked with idiotypic antisera. Specificity of the idiotypic antisera was tested in L-tyrosine-p-azobenzenearsonate-immune guinea pig LNC. Neither T- nor B-ABC were blocked in this heterologous system. Further blocking experiments were performed to characterize the nature of the T-ABC receptor. A variety of anti-Ig reagents, some of which block B-ABC, do not inhibit T-ABC suggesting that variable regions on T cells are not linked to Ig Constant regions.

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