Active and passive Na+ fluxes across the basolateral membrane of rabbit urinary bladder

1982 ◽  
Vol 67 (1) ◽  
pp. 219-229 ◽  
Author(s):  
Douglas C. Eaton ◽  
A. Michael Frace ◽  
S. Unglaub Silverthorn
Keyword(s):  
Urinary Bladder ◽  
Basolateral Membrane ◽  
Rabbit Urinary Bladder

Apical and basolateral membrane ionic channels in rabbit urinary bladder epithelium

1985 ◽  
Vol 405 (S1) ◽  
pp. S83-S88 ◽  
Author(s):  
Simon A. Lewis ◽  
John W. Hanrahan
Keyword(s):  
Urinary Bladder ◽  
Basolateral Membrane ◽  
Ionic Channels ◽  
Bladder Epithelium ◽  
Rabbit Urinary Bladder

Effect of hyperosmotic challenge on basolateral membrane potential in rabbit urinary bladder

1990 ◽  
Vol 258 (2) ◽  
pp. C248-C257 ◽  
Author(s):  
P. J. Donaldson ◽  
S. A. Lewis
Keyword(s):  
Membrane Potential ◽  
Urinary Bladder ◽  
Basolateral Membrane ◽  
Pump Current ◽  
Bathing Solution ◽  
Partial Recovery ◽  
Parallel Operation ◽  
Voltage Recovery ◽  
Rabbit Urinary Bladder ◽  
Basolateral Membrane Potential

In the rabbit urinary bladder, serosal hyperosmotic challenge (SHOC) with either 33 mM NaCl or 66 mM mannitol caused basolateral membrane potential (Vbl) to initially depolarize from -52.6 +/- 1.6 to -48.4 +/- 1.4 mV, followed by a recovery of Vbl to -57.5 +/- 1.3 mV after 13.7 +/- 1.0 min. The voltage recovery was dependent on both serosal HCO3- and Cl-, and in the absence of both, Vbl depolarized to -11.6 +/- 1.5 mV and the ratio of apical-to-basolateral resistance (Ra/Rbl) decreased from 21.0 +/- 3.4 to 8.3 +/- 3.1. This decrease in Ra/Rbl and consequent depolarization of Vbl is caused by a decrease in basolateral K+ conductance. Replacement of serosal Cl- with NO3- or SCN- followed by SHOC caused a sustained depolarization of Vbl to -32.5 +/- 4.4 and -40.9 +/- 0.9 mV, respectively. However, when Br- was used to replace Cl-, voltage recovery occurred but was slowed (24.0 +/- 2.7 min) and reduced in magnitude (-47.5 +/- 3.5 mV). Addition of amiloride (1 mM) or niflumic acid (100 microM), but not bumetanide (1 microM), to the serosal bathing solution inhibited voltage recovery causing Vbl to depolarize to -36.3 +/- 2.6 and -41.5 +/- 4.5 mV, respectively. Serosal addition of ouabain after SHOC caused Vbl to depolarize by 10.8 +/- 0.9 mV in 2 min. We speculate that the SHOC-induced initial depolarization of Vbl is a loss of Ba2(+)-sensitive K+ conductance caused by cell shrinkage. The subsequent repolarization/hyperpolarization of Vbl is caused by an enhanced basolateral membrane Na+ pump current and a reappearance of the Ba2(+)-sensitive K+ conductance. The parallel operation of Na(+)-H+ and Cl(-)-HCO3- exchanges will then supply Na+ for the pump current and, via cellular accumulation of Na+, K+, and Cl-, might result in a partial recovery of cell volume and thus Ba2(+)-sensitive K+ conductance.

Effects of serosal anion composition on the permeability properties of rabbit urinary bladder

1989 ◽  
Vol 256 (6) ◽  
pp. F1125-F1134
Author(s):  
P. J. Donaldson ◽  
L. K. Chen ◽  
S. A. Lewis
Keyword(s):  
Urinary Bladder ◽  
Basolateral Membrane ◽  
Anion Channel ◽  
Membrane Properties ◽  
Lag Phase ◽  
Na Transport ◽  
Transepithelial Na Transport ◽  
Bladder Replacement ◽  
Lag Period ◽  
Rabbit Urinary Bladder

This study describes the effects of serosal Cl- and HCO3- substitution on transepithelial Na+ transport and basolateral membrane properties of the rabbit urinary bladder. Replacement of Cl- with NO3-, SCN-, and Br- had no effect on transepithelial Na+ transport or the basolateral membrane potential (Vbl). However, gluconate, isethionate, and cyclamate (anions that were shown previously to be not as permeable as Cl- through the basolateral membrane anion channel), decreased transepithelial Na+ transport and depolarized Vbl. Replacement of HCO3- also produced a decrease in transepithelial Na+ transport and a depolarization of Vbl. Utilizing conventional and K+-specific microelectrodes, we found the depolarization to be due to a reduction in basolateral K+ conductance. This depolarization was reversible only when Cl- was returned to the serosal solution, the normally permeant anion NO3- being unable to affect repolarization, suggesting that both the K+ and Cl- conductance are reduced during depolarization. A lag period of some 4 min preceded the repolarization of Vbl. The Na+-H+ exchange blocker amiloride prolonged the lag phase associated with repolarization, whereas niflumic acid, a Cl-(-)HCO3- exchange blocker (in red blood cells) reduced the magnitude of Vbl repolarization. Because of the possible involvement of the exchangers it is believed that the lag phase represents a volume-dependent and/or pH-dependent reactivation of the basolateral membrane conductances.

Effect of Chronic Nitrofurantoin Administration on the Rabbit Urinary Bladder

1987 ◽  
Vol 137 (6) ◽  
Author(s):  
Philip M. Hanno ◽  
Beth Witkowski
Keyword(s):  
Urinary Bladder ◽  
Rabbit Urinary Bladder

Cyclical estrogen and free radical damage to the rabbit urinary bladder

2009 ◽  
Vol 21 (4) ◽  
pp. 489-494 ◽  
Author(s):  
Alexandra Rehfuss ◽  
Catherine Schuler ◽  
Christina Maxemous ◽  
Robert E. Leggett ◽  
Robert M. Levin
Keyword(s):  
Free Radical ◽  
Urinary Bladder ◽  
Free Radical Damage ◽  
Radical Damage ◽  
Rabbit Urinary Bladder

Effects of prostaglandins E1, E2 and F2α on changes in adenylate cyclase activity in rabbit urinary bladder

1993 ◽  
Vol 45 (3) ◽  
pp. 266-267
Author(s):  
Yoshiki Noda ◽  
Shun Kondo ◽  
Yohtalou Tashima ◽  
Takashi Morita
Keyword(s):  
Urinary Bladder ◽  
Adenylate Cyclase ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Rabbit Urinary Bladder

Dissociation of the metabolic from the contractile response to muscarinic stimulation in the rabbit urinary bladder

1988 ◽  
Vol 81 (2) ◽  
Author(s):  
MichaelR. Ruggieri ◽  
AlanJ. Wein ◽  
JospehA. Hypolite ◽  
RobertM. Levin
Keyword(s):  
Urinary Bladder ◽  
Contractile Response ◽  
Muscarinic Stimulation ◽  
Rabbit Urinary Bladder

Effects of Ganoderma Lucidum on Contractile Dysfunction of the Rabbit Urinary Bladder using an In-Vitro Model of Ischemia/Reperfusion

2018 ◽  
Vol 8 (1) ◽  
pp. 77-83
Author(s):  
Robert M. Levin ◽  
Li-Xia ◽  
Wu Wei ◽  
Catherine Schuler ◽  
Robert E. Leggett ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Ganoderma Lucidum ◽  
In Vitro Model ◽  
Ischemia Reperfusion ◽  
Contractile Dysfunction ◽  
Vitro Model ◽  
Rabbit Urinary Bladder

Developmental Factors in the Contractile Response of Rabbit Urinary Bladder: Effect of Anoxia and Extracellular Acidosis

Neonatology ◽  
1995 ◽  
Vol 67 (5) ◽  
pp. 370-375
Author(s):  
Jai-Young Yoon ◽  
Stephen A. Zderic ◽  
John W. Duckett ◽  
Howard M. Snyder, III ◽  
Robert M. Levin
Keyword(s):  
Urinary Bladder ◽  
Contractile Response ◽  
Developmental Factors ◽  
Extracellular Acidosis ◽  
Rabbit Urinary Bladder

scholarly journals Sesquiterpenoids of Atractylodes lancea root: Pharmacological effects in rabbit urinary bladder

1990 ◽  
Vol 52 ◽  
pp. 384
Author(s):  
Makoto Kobayashi ◽  
Masatsugu Iwamoto ◽  
Hifumi Ishikawa ◽  
Yoshiko Hirose ◽  
Johji Yamahara
Keyword(s):  
Urinary Bladder ◽  
Pharmacological Effects ◽  
Atractylodes Lancea ◽  
Rabbit Urinary Bladder
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