Rapid isolation of mitochondrial DNA. Mitochondrial DNA fromDrosophila serrata

P. Pissios; Z. G. Scouras

doi:10.1007/bf02118316

Rapid Isolation of Mitochondrial DNA-Depleted Mammalian Cells by Ethidium Bromide and Dideoxycytidine Treatments

Journal of Applied Biological Chemistry ◽

10.3839/jabc.2014.042 ◽

2014 ◽

Vol 57 (3) ◽

pp. 259-265 ◽

Cited By ~ 2

Author(s):

Young Geol Yoon ◽

Yoo Jin Oh ◽

Young Hyun Yoo

Keyword(s):

Mitochondrial Dna ◽

Ethidium Bromide ◽

Mammalian Cells ◽

Rapid Isolation

Rapid isolation of animal mitochondrial DNA in a small fixed-angle rotor at ultrahigh speed

Biochemical Genetics ◽

10.1007/bf00554547 ◽

1987 ◽

Vol 25 (5-6) ◽

pp. 385-390 ◽

Cited By ~ 33

Author(s):

Steven M. Carr ◽

O. Mitch Griffith

Keyword(s):

Mitochondrial Dna ◽

Fixed Angle ◽

Rapid Isolation

Rapid isolation of microsatellite DNAs and identification of polymorphic mitochondrial DNA regions in the fish rotan (Perccottus glenii) invading European Russia

Conservation Genetics Resources ◽

10.1007/s12686-015-0430-x ◽

2015 ◽

Vol 7 (2) ◽

pp. 363-368

Author(s):

T. L. King ◽

M. S. Eackles ◽

A. N. Reshetnikov

Keyword(s):

Mitochondrial Dna ◽

European Russia ◽

Perccottus Glenii ◽

Rapid Isolation

Rapid isolation of mitochondrial DNA fromDrosophila adults

Biochemical Genetics ◽

10.1007/bf02401791 ◽

1988 ◽

Vol 26 (5-6) ◽

pp. 381-386 ◽

Cited By ~ 8

Author(s):

J. M. Afonso ◽

J. Pestano ◽

M. Hernández

Keyword(s):

Mitochondrial Dna ◽

Rapid Isolation

Rapid isolation method of animal mitochondrial DNA by the alkaline lysis procedure

Biochemical Genetics ◽

10.1007/bf02395525 ◽

1988 ◽

Vol 26 (11-12) ◽

pp. 815-819 ◽

Cited By ~ 88

Author(s):

Koichiro Tamura ◽

Tadashi Aotsuka

Keyword(s):

Mitochondrial Dna ◽

Isolation Method ◽

Alkaline Lysis ◽

Rapid Isolation

Rapid isolation of animal mitochondrial DNA by alkaline extraction

FEBS Letters ◽

10.1016/0014-5793(85)80122-8 ◽

1985 ◽

Vol 192 (2) ◽

pp. 267-270 ◽

Cited By ~ 80

Author(s):

Tuula K. Palva ◽

E.Tapio Palva

Keyword(s):

Mitochondrial Dna ◽

Alkaline Extraction ◽

Rapid Isolation

Rapid isolation and purification of plastid and mitochondrial DNA from carrot cell suspensions

Plant Molecular Biology Reporter ◽

10.1007/bf02885645 ◽

1984 ◽

Vol 2 (3) ◽

pp. 32-36 ◽

Cited By ~ 11

Author(s):

Lorin R. DeBonte ◽

Benjamin F. Matthews

Keyword(s):

Mitochondrial Dna ◽

Cell Suspensions ◽

Isolation And Purification ◽

Rapid Isolation ◽

Carrot Cell

Technical Notes: Rapid Isolation of Fish Mitochondrial DNA with a Commercially Available Plasmid Isolation Kit

The Progressive Fish-Culturist ◽

10.1577/1548-8640(1993)055<0125:tnriof>2.3.co;2 ◽

1993 ◽

Vol 55 (2) ◽

pp. 125-127

Author(s):

Tuula K. Palva ◽

E. Tapio Palva

Keyword(s):

Mitochondrial Dna ◽

Rapid Isolation ◽

Plasmid Isolation

Evaluation of the dark field method for large association of spread DNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081814 ◽

1978 ◽

Vol 36 (2) ◽

pp. 190-191

Author(s):

Douglas C. Barker

Keyword(s):

Electron Microscopy ◽

Molecular Weight ◽

Mitochondrial Dna ◽

Extraction Method ◽

Field Method ◽

Dark Field ◽

Kinetoplast Dna ◽

Field Electron ◽

Field Electron Microscopy ◽

Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072733 ◽

1973 ◽

Vol 31 ◽

pp. 458-459

Author(s):

K. S. McCarty ◽

R. F. Weave ◽

L. Kemper ◽

F. S. Vogel

Keyword(s):

Mitochondrial Dna ◽

Ethidium Bromide ◽

Microscopic Examination ◽

Agaricus Bisporus ◽

Osmotic Shock ◽

Electron Microscopic Examination ◽

Electron Microscopic ◽

Isolated Mitochondria ◽

Dna Strands ◽

Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.