Rapid isolation method of animal mitochondrial DNA by the alkaline lysis procedure

1988 ◽  
Vol 26 (11-12) ◽  
pp. 815-819 ◽  
Author(s):  
Koichiro Tamura ◽  
Tadashi Aotsuka
Keyword(s):  
Mitochondrial Dna ◽  
Isolation Method ◽  
Alkaline Lysis ◽  
Rapid Isolation

scholarly journals Biased nicking of mitochondrial DNA during extraction can be avoided by choice of isolation method

2021 ◽  
Author(s):  
Bruno Marçal Repolês ◽  
Choco Michael Gorospe ◽  
Phong Tran ◽  
Anna Karin Nilsson ◽  
Paulina H. Wanrooij
Keyword(s):  
Skeletal Muscle ◽  
Mitochondrial Dna ◽  
Dna Isolation ◽  
Isolation Procedure ◽  
Isolation Method ◽  
Strand Breaks ◽  
Careful Choice ◽  
Isolation Methods ◽  
Solid Tissues ◽  
Long Range Pcr

AbstractThe integrity of mitochondrial DNA (mtDNA) isolated from solid tissues is critical for analyses such as long-range PCR. We show that a commonly-used DNA isolation procedure preferentially introduces strand breaks into the mtDNA extracted from the skeletal muscle of aged mice, while mtDNA from adult animals is less affected. We present a comparison of mtDNA isolation methods and identify one that avoids this biased loss of muscle mtDNA integrity. Our results highlight the importance of a careful choice of mtDNA isolation method and serve as a resource to researchers planning analysis of mtDNA isolated from solid tissues.

A Rapid Isolation Method for Bacillus coagulans from Rice Straw

2015 ◽  
Vol 43 (4) ◽  
pp. 401-404 ◽  
Author(s):  
Bitnara Lee ◽  
Hyundong Lee ◽  
Do-Won Jeong ◽  
Jong-Hoon Lee
Keyword(s):  
Rice Straw ◽  
Bacillus Coagulans ◽  
Isolation Method ◽  
Rapid Isolation

scholarly journals Rapid isolation method for radioactive strontium using Empore™ Strontium Rad Disk

2016 ◽  
Vol 16 (0) ◽  
pp. 15-21 ◽  
Author(s):  
Zijian Zhang ◽  
Kazuhiko Ninomiya ◽  
Naruto Takahashi ◽  
Takashi Saito ◽  
Kazuyuki Kita ◽  
...  
Keyword(s):  
Isolation Method ◽  
Rapid Isolation

scholarly journals The integrity and assay performance of tissue mitochondrial DNA is considerably affected by choice of isolation method

Mitochondrion ◽  
2021 ◽  
Author(s):  
Bruno Marçal Repolês ◽  
Choco Michael Gorospe ◽  
Phong Tran ◽  
Anna Karin Nilsson ◽  
Paulina H. Wanrooij
Keyword(s):  
Mitochondrial Dna ◽  
Isolation Method ◽  
Assay Performance
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