Rapid isolation method of animal mitochondrial DNA by the alkaline lysis procedure

1988 ◽  
Vol 26 (11-12) ◽  
pp. 815-819 ◽  
Author(s):  
Koichiro Tamura ◽  
Tadashi Aotsuka

2018 ◽  
Author(s):  
Ishwar Prakash Sharma ◽  
Narendra Kumar ◽  
A.K. Sharma


2021 ◽  
Author(s):  
Bruno Marçal Repolês ◽  
Choco Michael Gorospe ◽  
Phong Tran ◽  
Anna Karin Nilsson ◽  
Paulina H. Wanrooij

AbstractThe integrity of mitochondrial DNA (mtDNA) isolated from solid tissues is critical for analyses such as long-range PCR. We show that a commonly-used DNA isolation procedure preferentially introduces strand breaks into the mtDNA extracted from the skeletal muscle of aged mice, while mtDNA from adult animals is less affected. We present a comparison of mtDNA isolation methods and identify one that avoids this biased loss of muscle mtDNA integrity. Our results highlight the importance of a careful choice of mtDNA isolation method and serve as a resource to researchers planning analysis of mtDNA isolated from solid tissues.





2015 ◽  
Vol 43 (4) ◽  
pp. 401-404 ◽  
Author(s):  
Bitnara Lee ◽  
Hyundong Lee ◽  
Do-Won Jeong ◽  
Jong-Hoon Lee


2016 ◽  
Vol 16 (0) ◽  
pp. 15-21 ◽  
Author(s):  
Zijian Zhang ◽  
Kazuhiko Ninomiya ◽  
Naruto Takahashi ◽  
Takashi Saito ◽  
Kazuyuki Kita ◽  
...  




Mitochondrion ◽  
2021 ◽  
Author(s):  
Bruno Marçal Repolês ◽  
Choco Michael Gorospe ◽  
Phong Tran ◽  
Anna Karin Nilsson ◽  
Paulina H. Wanrooij


1992 ◽  
Vol 48 (7) ◽  
pp. 671-673 ◽  
Author(s):  
P. Pissios ◽  
Z. G. Scouras


The Analyst ◽  
2000 ◽  
Vol 125 (4) ◽  
pp. 651-656 ◽  
Author(s):  
Eugene C. Yi ◽  
Murray Hackett


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