Rapid isolation and purification of plastid and mitochondrial DNA from carrot cell suspensions

1984 ◽  
Vol 2 (3) ◽  
pp. 32-36 ◽  
Author(s):  
Lorin R. DeBonte ◽  
Benjamin F. Matthews
Keyword(s):  
Mitochondrial Dna ◽  
Cell Suspensions ◽  
Isolation And Purification ◽  
Rapid Isolation ◽  
Carrot Cell

Xylose transfer to lipids by membranes of carrot cell suspensions

1993 ◽  
Vol 33 (6) ◽  
pp. 1347-1352 ◽  
Author(s):  
Luis A. Quesada-Allué ◽  
Karin Hagelin ◽  
Fiorella LoSchiavo
Keyword(s):  
Cell Suspensions ◽  
Carrot Cell

Rapid Isolation of Mitochondrial DNA-Depleted Mammalian Cells by Ethidium Bromide and Dideoxycytidine Treatments

2014 ◽  
Vol 57 (3) ◽  
pp. 259-265 ◽  
Author(s):  
Young Geol Yoon ◽  
Yoo Jin Oh ◽  
Young Hyun Yoo
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Mammalian Cells ◽  
Rapid Isolation

Importance of actin cytoskeleton behaviour during preservation of carrot cell suspensions in liquid nitrogen

PROTOPLASMA ◽  
1993 ◽  
Vol 173 (1-2) ◽  
pp. 35-47 ◽  
Author(s):  
Christiane Morisset ◽  
C. Gazeau ◽  
J. Hansz ◽  
J. Dereuddre
Keyword(s):  
Actin Cytoskeleton ◽  
Liquid Nitrogen ◽  
Cell Suspensions ◽  
Carrot Cell

A Modified Procedure for Isolation of Yeast Mitochondrial DNA

2002 ◽  
Vol 57 (9-10) ◽  
pp. 960-962 ◽  
Author(s):  
Trayana Nedeva ◽  
Ventzislava Petrova ◽  
Tsonka Hristozova ◽  
Anna Kujumdzieva
Keyword(s):  
Cell Wall ◽  
Mitochondrial Dna ◽  
Molecular Analysis ◽  
Nutrient Medium ◽  
Mitochondrial Fraction ◽  
Batch Cultivation ◽  
Exponential Growth Phase ◽  
Isolation And Purification ◽  
Cell Wall Preparation ◽  
Enzymatic Lysis

A modified, rapid and inexpensive method for preparation of mitochondrial DNA (mtDNA), suitable for molecular analysis is proposed. It comprises batch cultivation of Saccharomyces cerevisiae strain NBIMCC 583 on a simple nutrient medium at 28 °C; permeabialization of cells from late exponential growth phase with cetyltrimethylamonnium bromide, mechanical disintegration of the cell wall; preparation of a mitochondrial fraction and subsequent isolation and purification of mtDNA. The amount and the purity of the obtained mtDNA have been checked and its application for molecular analysis proven.The main advantages of the proposed procedure for isolation of mtDNA are introduction of simple nutrient medium, replacement of the enzymatic lysis of the cell wall by the cheaper mechanical one, avoidance of ultracentrifugation steps and use of harmful chemical substances.

Rapid isolation and purification of antibody to factor VIII by protein A

1979 ◽  
Vol 14 (6) ◽  
pp. 925-930 ◽  
Author(s):  
Helen Lee ◽  
Derek Tucker ◽  
J.P. Allain
Keyword(s):  
Factor Viii ◽  
Protein A ◽  
Isolation And Purification ◽  
Rapid Isolation

Preparation of monomeric and polymeric β-cyclodextrin functionalized monoliths for rapid isolation and purification of puerarin from Radix puerariae

2011 ◽  
Vol 34 (16-17) ◽  
pp. 2131-2137 ◽  
Author(s):  
Yongqin Lv ◽  
Timothy C. Hughes ◽  
Xiaojuan Hao ◽  
Danping Mei ◽  
Tianwei Tan
Keyword(s):  
Isolation And Purification ◽  
Rapid Isolation ◽  
Radix Puerariae

scholarly journals A standardized method to purify cardiomyocytes from individual mouse hearts of any age

2021 ◽  
Author(s):  
Amy M Nicks ◽  
Sara R Holman ◽  
Andrea Y Chan ◽  
Michael Tsang ◽  
Paul E Young ◽  
...  
Keyword(s):  
Heart Development ◽  
Enzymatic Digestion ◽  
High Yield ◽  
Coronary Perfusion ◽  
Isolation And Purification ◽  
Immunomagnetic Bead ◽  
Rapid Isolation ◽  
Neonatal Cardiomyocytes ◽  
Standardized Method

Rationale: Primary cardiomyocytes are invaluable for understanding postnatal heart development and elucidating disease mechanisms in genetic and pharmacological models, however, a method to obtain freshly purified cardiomyocytes at any postnatal age, without using different age-dependent isolation procedures and cell culture, is lacking. Objective: To develop a standardized method that allows rapid isolation and purification of cardiomyocytes in high yield and viability from individual neonatal, infant, and adult mice. Methods and Results: Hearts of C57BL/6J mice were cannulated using a novel in situ aortic cannulation procedure optimized to allow cannulation of even the very small vessel of neonates (postnatal day 0-2, P0-2). Hearts were then subjected to Langendorff retrograde perfusion and enzymatic digestion. Cardiomyocytes were isolated after subsequent tissue disaggregation and filtration, in high yield (1.56-2.2x106 cardiomyocytes/heart) and viability (~70-100%). The larger size of infant (P10 and P13) and adult (P70), but not neonatal, cardiomyocytes relative to non-myocytes, allowed enrichment by differential centrifugation. Cardiomyocytes from all ages were further purified by immunomagnetic bead-based depletion of non-myocytes. Together, these procedures resulted in the isolation of highly purified cardiomyocytes (~94%) within 1 hour, enabling experiments using individual replicates. For example, RNA-sequencing of cardiomyocytes purified from one P2 male and female heart per litter (n=4 litters) showed distinct clustering by litters and sex differences for nine differentially expressed genes (FDR<0.005). In situ fixation via coronary perfusion, performed immediately after tissue digestion, preserved the cytoarchitecture of isolated cardiomyocytes (yielding ~94% rod-shaped cardiomyocytes at all ages), allowing capture of spindle-shaped neonatal cells undergoing mitosis, as well as enabling accurate quantitation of cardiomyocyte area and nucleation state. Conclusion: The procedures developed here provide a universal protocol for the rapid isolation and purification of high-quality cardiomyocytes from hearts of any postnatal age, even those of neonates, thereby enabling direct comparisons between individual hearts.

scholarly journals Rapid Isolation and Purification of Secreted Bacteriocins from Streptococcus mutans and Other Lactic Acid Bacteria

BIO-PROTOCOL ◽  
2020 ◽  
Vol 10 (22) ◽  
Author(s):  
Marcus Cheng ◽  
Siew-Ging Gong ◽  
Céline Lévesque
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Streptococcus Mutans ◽  
Isolation And Purification ◽  
Rapid Isolation

scholarly journals Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

10.3791/51682 ◽  
2014 ◽  
Author(s):  
Janine M. Preble ◽  
Christina A. Pacak ◽  
Hiroshi Kondo ◽  
Allison A. MacKay ◽  
Douglas B. Cowan ◽  
...  
Keyword(s):  
Isolation And Purification ◽  
Rapid Isolation ◽  
Tissue Dissociation ◽  
Differential Filtration
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