scholarly journals Transcription initiation site of a NADP-specific glutamate dehydrogenase gene and potential use of its promoter region to express foreign genes in ammonium-culturedChlorella sorokiniana cells

1994 ◽  
Vol 6 (4) ◽  
pp. 437-438
Author(s):  
Philip W. Miller ◽  
Brenda L. Russell ◽  
Robert R. Schmidt
Keyword(s):  
Glutamate Dehydrogenase ◽  
Transcription Initiation ◽  
Promoter Region ◽  
Initiation Site ◽  
Transcription Initiation Site ◽  
Dehydrogenase Gene ◽  
Foreign Genes ◽  
Potential Use

Structural and Functional Analysis of the Promoter Region of the Human MCP-3 Gene: Transactivation of Expression by Novel Recognition Sequences Adjacent to the Transcription Initiation Site

1997 ◽  
Vol 16 (2) ◽  
pp. 173-183 ◽  
Author(s):  
KEIICHI MURAKAMI ◽  
HISAYUKI NOMIYAMA ◽  
RETSU MIURA ◽  
ANJA FOLLENS ◽  
PIERRE FITEN ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Transcription Initiation ◽  
Promoter Region ◽  
Initiation Site ◽  
Transcription Initiation Site

scholarly journals Sp1 and Sp3 control constitutive expression of the human NHE2 promoter by interactions with the proximal promoter and the transcription initiation site

2007 ◽  
Vol 407 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Ian Pearse ◽  
Ying X. Zhu ◽  
Eleanor J. Murray ◽  
Pradeep K. Dudeja ◽  
Krishnamurthy Ramaswamy ◽  
...  
Keyword(s):  
Transcription Initiation ◽  
Promoter Region ◽  
Promoter Activity ◽  
Critical Role ◽  
Constitutive Expression ◽  
Regulatory Elements ◽  
Initiation Site ◽  
Transcription Initiation Site ◽  
Proximal Promoter ◽  
Gc Box

We have previously cloned the human Na+/H+ exchanger NHE2 gene and its promoter region. In the present study, the regulatory elements responsible for the constitutive expression of NHE2 were studied. Transient transfection assays revealed that the −40/+150 promoter region contains the core promoter responsible for the optimal promoter activity. A smaller fragment, −10/+40, containing the TIS (transcription initiation site) showed minimal activity. We identified a palindrome that overlaps the TIS and binds to the transcription factors Sp1 and Sp3. Mutations in the 5′ flank of the palindrome abolished the Sp1/Sp3 interaction and reduced promoter activity by approx. 45%. In addition, a conserved GC-box centered at −25 was found to play a critical role in basal promoter activity and also interacted with Sp1 and Sp3. An internal deletion in the GC-box severely reduced the promoter activity. Sp1/Sp3 binding to these elements was established using gel-mobility shift assays, confirmed by chromatin immunoprecipitation and co-transfections in Drosophila SL2 cells. Furthermore, we identified two positive regulatory elements in the DNA region corresponding to the 5′-UTR (5′-untranslated region). The results in the present study indicate that Sp1 and Sp3 are required for constitutive NHE2 expression and that the positive regulatory elements of the 5′-UTR may co-operate with the 5′-flanking region to achieve the optimal promoter activity.

scholarly journals Characterization of the transcription-initiation site and of the promoter region within the 5' flanking region of the human aldolase C gene

1990 ◽  
Vol 192 (3) ◽  
pp. 805-811 ◽  
Author(s):  
Pasqualina BUONO ◽  
Francesco P. MANCINI ◽  
Paola IZZO ◽  
Francesco SALVATORE
Keyword(s):  
Transcription Initiation ◽  
Promoter Region ◽  
Initiation Site ◽  
Transcription Initiation Site ◽  
Aldolase C ◽  
Flanking Region

A transcription initiation site for the hepatitis B virus X gene is directed by the promoter-binding protein.

1993 ◽  
Vol 67 (5) ◽  
pp. 2559-2565 ◽  
Author(s):  
K Yaginuma ◽  
I Nakamura ◽  
S Takada ◽  
K Koike
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Transcription Initiation ◽  
Binding Protein ◽  
Initiation Site ◽  
Transcription Initiation Site ◽  
B Virus ◽  
X Gene

scholarly journals Isolation of novel single-chain Cro proteins targeted for binding to the bcl-2 transcription initiation site by repertoire selection and subunit combinatorics

2005 ◽  
Vol 18 (11) ◽  
pp. 537-546 ◽  
Author(s):  
Kristina Jonas ◽  
Erhard Van Der Vries ◽  
Mikael T.I. Nilsson ◽  
Mikael Widersten
Keyword(s):  
Transcription Initiation ◽  
Initiation Site ◽  
Transcription Initiation Site ◽  
Single Chain ◽  
Repertoire Selection

Identification of the transcription initiation site of the asexually expressed rRNA genes of the malaria parasite Plasmodium berghei

1999 ◽  
Vol 99 (2) ◽  
pp. 193-205 ◽  
Author(s):  
Rosalina M.L. van Spaendonk ◽  
Glenn A. McConkey ◽  
Jai Ramesar ◽  
Andrei Gabrielian ◽  
Thomas F. McCutchan ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Transcription Initiation ◽  
Malaria Parasite ◽  
Initiation Site ◽  
Transcription Initiation Site ◽  
Rrna Genes ◽  
Parasite Plasmodium
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