Spectrum of spontaneous missense mutations causing cyclic AMP-resistance phenotypes in cultured S49 mouse lymphoma cells differs markedly from those of mutations induced by alkylating mutagens

1994 ◽  
Vol 20 (4) ◽  
pp. 301-311 ◽  
Author(s):  
Karen B. Gorman ◽  
Robert A. Steinberg
Keyword(s):  
Cyclic Amp ◽  
Missense Mutations ◽  
Lymphoma Cells ◽  
Mouse Lymphoma Cells ◽  
Mouse Lymphoma ◽  
Alkylating Mutagens

Interactions between cyclic AMP- and phorbol ester-dependent phosphorylation systems in S49 mouse lymphoma cells

1985 ◽  
Vol 125 (2) ◽  
pp. 200-206 ◽  
Author(s):  
Zolt�;n Kiss ◽  
Robert A. Steinberg
Keyword(s):  
Cyclic Amp ◽  
Phorbol Ester ◽  
Lymphoma Cells ◽  
Mouse Lymphoma Cells ◽  
Mouse Lymphoma

A variant of S49 mouse lymphoma cells with enhanced secretion of cyclic AMP

1979 ◽  
Vol 100 (3) ◽  
pp. 579-588 ◽  
Author(s):  
Robert A. Steinberg ◽  
M. Gwen Steinberg ◽  
Theodoor Van Daalen Wetters
Keyword(s):  
Cyclic Amp ◽  
Lymphoma Cells ◽  
Mouse Lymphoma Cells ◽  
Enhanced Secretion ◽  
Mouse Lymphoma

ATP enhances cyclic AMP accumulation by intact P388 mouse lymphoma cells

1980 ◽  
Vol 630 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Sobhy R. Ayad ◽  
Richard J. Hughes
Keyword(s):  
Cyclic Amp ◽  
Lymphoma Cells ◽  
Cyclic Amp Accumulation ◽  
Mouse Lymphoma Cells ◽  
Mouse Lymphoma

scholarly journals A kinase-negative mutant of S49 mouse lymphoma cells is defective in posttranslational maturation of catalytic subunit of cyclic AMP-dependent protein kinase.

1991 ◽  
Vol 11 (2) ◽  
pp. 705-712 ◽  
Author(s):  
R A Steinberg
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Sodium Dodecyl ◽  
Wild Type ◽  
Lymphoma Cells ◽  
Dependent Protein Kinase ◽  
Mouse Lymphoma Cells ◽  
C Subunit ◽  
Dependent Protein ◽  
Mouse Lymphoma

Kinase-negative mutants of S49 mouse lymphoma cells, which lack detectable catalytic (C) subunit of cyclic AMP-dependent protein kinase, nevertheless contain cytoplasmic mRNAs for the two major forms of C subunit, C alpha and C beta. Investigation of the metabolism of C subunits in wild-type and mutant cells was undertaken to identify the step(s) at which C subunit expression was defective in kinase-negative cells. [35S]methionine-labeled C subunits from cytosolic fractions of wild-type S49 cells or C subunit-overexpressing cell lines were visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after purification by either affinity chromatography using a peptide inhibitor of C subunit as the ligand or immunoadsorption with an anti-C subunit antiserum. Immunoadsorption revealed electrophoretic forms of C alpha and C beta subunits that migrated faster than those detected in affinity-purified samples; this unexpected heterogeneity suggested that functional activation of C subunit may require posttranslational modification. Immunoadsorption of cytosolic fractions from wild-type cells labeled for various times with [35S]methionine revealed an additional posttranslational maturation step. The bulk of immunoadsorbable C subunit label in cells pulse-labeled for 5 min or less was in an insoluble fraction from which it could be solubilized with a detergent-containing buffer; solubilization of the newly synthesized material proceeded over an incubation period of about 10 min. The primary defect in kinase-negative cells appeared to be in this solubilization step, since about equal C subunit radioactivity was found in detergent extracts of wild-type and kinase-negative cells but very little was found in mutant cytosols. I speculate that an accessory factor required for proper folding of newly synthesized C subunit in defective in the kinase-negative cells.

A kinase-negative mutant of S49 mouse lymphoma cells is defective in posttranslational maturation of catalytic subunit of cyclic AMP-dependent protein kinase

1991 ◽  
Vol 11 (2) ◽  
pp. 705-712
Author(s):  
R A Steinberg
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Sodium Dodecyl ◽  
Wild Type ◽  
Lymphoma Cells ◽  
Dependent Protein Kinase ◽  
Mouse Lymphoma Cells ◽  
C Subunit ◽  
Dependent Protein ◽  
Mouse Lymphoma

Kinase-negative mutants of S49 mouse lymphoma cells, which lack detectable catalytic (C) subunit of cyclic AMP-dependent protein kinase, nevertheless contain cytoplasmic mRNAs for the two major forms of C subunit, C alpha and C beta. Investigation of the metabolism of C subunits in wild-type and mutant cells was undertaken to identify the step(s) at which C subunit expression was defective in kinase-negative cells. [35S]methionine-labeled C subunits from cytosolic fractions of wild-type S49 cells or C subunit-overexpressing cell lines were visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after purification by either affinity chromatography using a peptide inhibitor of C subunit as the ligand or immunoadsorption with an anti-C subunit antiserum. Immunoadsorption revealed electrophoretic forms of C alpha and C beta subunits that migrated faster than those detected in affinity-purified samples; this unexpected heterogeneity suggested that functional activation of C subunit may require posttranslational modification. Immunoadsorption of cytosolic fractions from wild-type cells labeled for various times with [35S]methionine revealed an additional posttranslational maturation step. The bulk of immunoadsorbable C subunit label in cells pulse-labeled for 5 min or less was in an insoluble fraction from which it could be solubilized with a detergent-containing buffer; solubilization of the newly synthesized material proceeded over an incubation period of about 10 min. The primary defect in kinase-negative cells appeared to be in this solubilization step, since about equal C subunit radioactivity was found in detergent extracts of wild-type and kinase-negative cells but very little was found in mutant cytosols. I speculate that an accessory factor required for proper folding of newly synthesized C subunit in defective in the kinase-negative cells.

scholarly journals Cyclic AMP stimulates dephosphorylation of specific proteins in intact S49 mouse lymphoma cells

FEBS Letters ◽  
1985 ◽  
Vol 180 (2) ◽  
pp. 207-211 ◽  
Author(s):  
Zoltán Kiss ◽  
Robert A. Steinberg
Keyword(s):  
Cyclic Amp ◽  
Lymphoma Cells ◽  
Mouse Lymphoma Cells ◽  
Specific Proteins ◽  
Mouse Lymphoma
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