Basal plasma-catecholamine-level determination using HPLC-ED and different sample cleanup techniques

1988 ◽  
Vol 25 (3) ◽  
pp. 223-229 ◽  
Author(s):  
B. Dirks ◽  
C. Vorwalter ◽  
A. Grünert ◽  
F. W. Ahnefeld
Keyword(s):  
Plasma Catecholamine ◽  
Catecholamine Level ◽  
Basal Plasma ◽  
Plasma Catecholamine Level

Sensitivities of cardiac O2 consumption and contractility to catecholamines in dogs

1991 ◽  
Vol 261 (1) ◽  
pp. H196-H205 ◽  
Author(s):  
Y. Ohgoshi ◽  
Y. Goto ◽  
S. Futaki ◽  
H. Yaku ◽  
H. Suga
Keyword(s):  
Mechanical Energy ◽  
Plasma Catecholamines ◽  
Systolic Pressure ◽  
Plasma Catecholamine ◽  
Catecholamine Level ◽  
Oxygen Cost ◽  
O2 Consumption ◽  
Total Mechanical Energy ◽  
Plasma Catecholamine Level ◽  
Stimulation Of

We studied the effects of plasma catecholamines from the adrenal gland on systolic pressure-volume area (PVA)-independent O2 consumption (VO2) and contractility index (Emax) in the left ventricle of excised cross-circulated dog hearts. PVA is a measure of the total mechanical energy of contraction. Under baseline conditions, the PVA-independent VO2 correlated with plasma catecholamine level in the hearts (r = 0.84). Plasma epinephrine and norepinephrine levels increased gradually from 0.3 and 0.4 ng/ml to 10.3 and 2.7 ng/ml on average during adrenal sympathetic nerve stimulation of support dogs. Simultaneously, Emax and PVA-independent VO2 increased by 240 +/- 127 (SD) and 75 +/- 24%. Although their increases were monotonic in a given heart, their sensitivities to catecholamines were considerably variable among hearts. However, these two sensitivities were correlated (r = 0.96) with each other in the hearts, and the interheart variation of the sensitivity of the PVA-independent VO2 to Emax (i.e., oxygen cost of Emax) was smaller. We conclude that the oxygen cost of Emax is less variable among hearts despite large interheart variations of Emax and VO2 responses to plasma catecholamines.

Plasma catecholamine level and portal venous pressure as guides to prognosis in patients with cirrhosis

1988 ◽  
Vol 6 (3) ◽  
pp. 350-358 ◽  
Author(s):  
U. Tage-Jensen ◽  
J.H. Henriksen ◽  
E. Christensen ◽  
A. Widding ◽  
H. Ring-Larsen ◽  
...  
Keyword(s):  
Venous Pressure ◽  
Portal Venous Pressure ◽  
Plasma Catecholamine ◽  
Catecholamine Level ◽  
Plasma Catecholamine Level

Change of Hemodynamic Parameters and Plasma Catecholamine Level during Laparoscopic Cholecystectomy

1992 ◽  
Vol 25 (3) ◽  
pp. 515
Author(s):  
Young In Kim ◽  
Mi Ae Shur ◽  
Hee Soon Kim ◽  
Sung Jin Hong ◽  
Se Ho Moon ◽  
...  
Keyword(s):  
Laparoscopic Cholecystectomy ◽  
Plasma Catecholamine ◽  
Catecholamine Level ◽  
Hemodynamic Parameters ◽  
Plasma Catecholamine Level

Mechanisms of initial heart rate response to postural change

1982 ◽  
Vol 243 (5) ◽  
pp. H676-H681 ◽  
Author(s):  
C. Borst ◽  
W. Wieling ◽  
J. F. van Brederode ◽  
A. Hond ◽  
L. G. de Rijk ◽  
...  
Keyword(s):  
Heart Rate ◽  
Time Course ◽  
Heart Rate Response ◽  
Cardiovascular Effects ◽  
Plasma Catecholamine ◽  
Catecholamine Level ◽  
Free Standing ◽  
Leg Muscles ◽  
Plasma Catecholamine Level ◽  
Head Up Tilt

We explored in 43 healthy subjects the afferent mechanisms of the initial heart rate response to standing by comparing free standing, 70 degrees head-up tilt, handgrip, and contraction of abdominal and leg muscles. The results indicate the following. 1) Standing evokes an immediate, large, bimodal increase of heart rate (HR) of about 20 s duration that far exceeds the gradual HR rise induced by 70 degrees head-up tilt. 2) The immediate HR increase with active standing is due to the exercise reflex and results in a first peak about 3 s after standing briskly. 3) The secondary, more gradual HR increase after 5 s of standing and the subsequent rapid decrease of HR between about 12 and 20 s corresponds through the baroreceptor reflex with a striking fall, recovery, and sometimes overshoot of arterial pressure. 4) The maximum HR increase found after about 12 s of standing is augmented and delayed after rest. 5) The time course of the initial HR response is not modified by physical training. We conclude that active and passive changes of posture result in fundamentally different cardiovascular effects for about 20 s and that "central command," muscle receptors, high-pressure receptors, low-pressure receptors, and the plasma catecholamine level are probably all involved in the initial HR response to standing.

Plasma Catecholamine Levels and Vascular Response in Deoxycorticosterone Acetate Hypertension of Rats

1980 ◽  
Vol 59 (s6) ◽  
pp. 315s-317s ◽  
Author(s):  
W. Rascher ◽  
R. Dietz ◽  
A. Schomig ◽  
J. Weber ◽  
F. Gross
Keyword(s):  
Hind Limb ◽  
Vascular Tone ◽  
Plasma Concentrations ◽  
Plasma Catecholamine ◽  
Vascular Response ◽  
Deoxycorticosterone Acetate ◽  
Pronounced Increase ◽  
Basal Plasma ◽  
Noradrenaline And Adrenaline ◽  
Catecholamine Levels

1. In rats with deoxycorticosterone acetate (DOCA) hypertension basal plasma concentrations of noradrenaline and adrenaline correspond to those of sham-treated controls. 2. In DOCA-treated rats frusemide caused a more pronounced increase in plasma noradrenaline than in control rats. This difference was not observed for adrenaline. 3. In the isolated perfused hind-limb preparation the sensitivity to noradrenaline was already enhanced before blood pressure was elevated. 4. These results suggest that the adrenergic vascular tone is increased in DOCA hypertension in rats.

scholarly journals Headache as a Predictive Factor of Severe Systolic Hypertension in Acute Ischemic Stroke

2003 ◽  
Vol 30 (3) ◽  
pp. 210-214 ◽  
Author(s):  
Yoon-Ho Hong ◽  
Yong-Seok Lee ◽  
Seong-Ho Park
Keyword(s):  
Logistic Regression ◽  
Ischemic Stroke ◽  
Cerebral Infarction ◽  
Acute Ischemic Stroke ◽  
Acute Cerebral Infarction ◽  
Stroke Severity ◽  
Plasma Catecholamine ◽  
Catecholamine Level ◽  
Stroke Subtypes ◽  
The Mean

ABSTRACT:Background:Elevation of blood pressure (BP) is common in acute cerebral infarction, with several studies reporting a high plasma catecholamine level or previous hypertension as a contributory factor. However, more comprehensive studies on associated clinical parameters are lacking. Our main aim in undertaking this study was to correlate clinical variables associated with a BPelevation in acute ischemic stroke.Methods:Consecutive patients who were admitted to the emergency room and diagnosed with an acute cerebral infarction within 24 hours after the onset of symptoms were investigated. A BP elevation was defined as a high systolic (³200mmHg) or diastolic (³110 mmHg) pressure. The mean systolic and diastolic BP were compared between the different stroke subtypes, lesion locations (carotid vs. vertebrobasilar), and hemispheric sides. The frequency of symptoms, risk factors, location of the infarct, stroke severity, vascular status and laboratory abnormalities were analyzed in order to build a regression model.Results:One hundred thirty-one patients were recruited (M:F=60:71, mean age 66±12 years) and an elevated BP was identified in 33 patients (25.2%). The mean systolic and diastolic BP did not differ significantly between the stroke subtypes, lesion locations, and hemispheric sides. According to univariate logistic regression, an elevated systolic BP correlated with headache (p=0.01) and underlying hypertension (p=0.02) while an elevated diastolic BP correlated with underlying hypertension (p=0.01). Multivariate logistic regression analysis revealed previous hypertension (OR 5.21, 95% CI 1.40-19.37) and headache (OR 4.09, 95% CI 1.44-11.66) to be independent predictors of an elevated systolic BP.Conclusions:Headache itself is closely associated with severe systolic BP elevation in acute ischemic stroke. Whether treatment of elevated BP improves headache and clinical outcome is not yet known, necessitating future controlled studies.

Comparison of double- and single-isotope enzymatic derivative methods for measuring catecholamines in human plasma.

1978 ◽  
Vol 24 (4) ◽  
pp. 567-570 ◽  
Author(s):  
M I Evans ◽  
J B Halter ◽  
D Porte
Keyword(s):  
Plasma Catecholamines ◽  
Assay Method ◽  
Plasma Catecholamine ◽  
Isotope Method ◽  
Coefficients Of Variation ◽  
Basal Plasma ◽  
Isotope Technique ◽  
Assay Time ◽  
Plasma Catecholamine Concentrations ◽  
Double Isotope

Abstract We directly compared the reliability of a single-isotope enzymatic derivative technique for measurement of plasma catecholamines with that of the well-established double-isotope method. A significant (p less than 0.001) correlation was observed between measurements (n = 52) in the two assays, both for norepinephrine (r = 0.97) and epinephrine (r = 0.80). Means and coefficients of variation for the two analytes in a pooled specimen of plasma, measured repeatedly during six months, were virtually identical by each assay method. Basal plasma catecholamine concentrations in two different groups of apparently healthy subjects were also similar by each method. Dopamine concentrations in plasma were consistently below the limits measurable by either technique. The single-isotope assay requires half the assay time and 1/200th the sample as the double-isotope method. We conclude that this assay is just as reliable as the double-isotope technique and gives virtually identical values for norepinephrine and epinephrine concentrations in the physiological range.

Plasma and 24 H-Urinary Catecholamine Concentrations in Normal and Patient Populations

1993 ◽  
Vol 30 (1) ◽  
pp. 38-44 ◽  
Author(s):  
G A Ross ◽  
E C Newbould ◽  
J Thomas ◽  
P M G Bouloux ◽  
G M Besser ◽  
...  
Keyword(s):  
Clinical Investigation ◽  
Plasma Catecholamine ◽  
Reference Ranges ◽  
Confidence Limits ◽  
Urinary Catecholamines ◽  
Hospital Patients ◽  
Basal Plasma ◽  
Noradrenaline And Adrenaline ◽  
Positive Results ◽  
Repeat Sampling

Using a single HPLC-ECD methodology, plasma catecholamine data were compiled from 545 patients and 144 healthy subjects, and 24 h urinary free catecholamine data from 106 patients. From these data, normal reference ranges were derived for the clinical investigation of suspected phaeochromocytomas, specifically to address the question as to when a result is normal and when further investigation may be required. We have shown that noradrenaline and adrenaline concentrations in plasma and urine are not normally distributed, and basal plasma noradrenaline is significantly lower in normal volunteers than in hospital patients. We have also demonstrated that a reference range which does not take account of these factors will give a significant number of false-positive results. Age and sex did not appear to be significant variables for either plasma or urinary catecholamines. In the investigation of phaeochromocytoma, 95% confidence limits may be used as a warning to repeat sampling and 99% confidence limits as rendering the diagnosis extremely probable. In patients with phaeochromocytomas, urinary catecholamines had a 99·5% specificity and 100% sensitivity in the diagnosis, and thus provide for a rapid and efficient diagnostic screen.

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