A novel bioaction of PAF: Induction of microbicidal activity in guinea pig bone marrow cells

Lipids ◽  
1988 ◽  
Vol 23 (12) ◽  
pp. 1119-1124 ◽  
Author(s):  
Hidetoshi Hayashi ◽  
Ichiro Kudo ◽  
Toshiyuki Kato ◽  
Ryushi Nozawa ◽  
Shoshichi Nojima ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Bone Marrow Cells ◽  
Microbicidal Activity ◽  
Marrow Cells

Guinea pig bone marrow cells treated with platelet-activating factor generate factor(s) which affects their DNA synthesis and microbicidal activity

Lipids ◽  
1991 ◽  
Vol 26 (12) ◽  
pp. 1065-1070 ◽  
Author(s):  
Ichiro Kudo ◽  
Toshiyuki Kato ◽  
Hidetoshi Hayashi ◽  
Ryohei Yanoshita ◽  
Koichi Ikizawa ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Platelet Activating Factor ◽  
Microbicidal Activity ◽  
Marrow Cells

Induction by platelet activating factor of candidacidal activity in guinea pig bone marrow cells

1988 ◽  
Vol 10 ◽  
pp. 19
Author(s):  
I. Kudo ◽  
H. Hayashi ◽  
T. Kato ◽  
R. Nozawa ◽  
S. Nojima ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Bone Marrow Cells ◽  
Platelet Activating Factor ◽  
Marrow Cells

scholarly journals Buoyant density separation of cells. I. The buoyant distribution of guinea pig bone marrow cells.

1975 ◽  
Vol 23 (5) ◽  
pp. 378-389 ◽  
Author(s):  
R C Leif ◽  
S B Smith ◽  
L A Dunlap ◽  
S B Leif
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Bone Marrow Cells ◽  
Buoyant Density ◽  
Cell Types ◽  
Density Fractions ◽  
Density Distributions ◽  
First Approximation ◽  
The Individual ◽  
Marrow Cells

Guinea pig bone marrow cells were separated by buoyant density utilizing linear gradients of bovine serum albumin (BSA). It has finally become possible to characterize the cells present in the density fractions in terms of classical morphology. The development of the Cell Type computer program which calculates the percentages of the individual types of cells present in the fractions and their buoyant density distributions and plots the data has greatly facilitated and improved the accuracy of these studies. Approximately 40 cell types were observed in guinea pig bone marrow. Cells with definitive morphologies such as erythrocytes, the neutrophilic series, the binucleate blast megakaryocyte precursor and cells in mitosis band as virtually single peaks. Cells which are parts of continua or can easily be wrongly classified are found in multiple peaks. The small lymphocytes which are known to be polydisperse are found as five peaks. Because of the very strong benzidine staining by the glutaraldehyde-fixed hemoglobin, some of the erythroblasts were wrongly staged, resulting in a multimodal distribution. The presence of macrocytes further complicated these distributions. The rule that the younger cells are always less dense than the mature cells was adhered to in those cases where the cells could be definitively characterized, such as the neutrophilic series and the blasts. These results indicate that morphology is a good first approximation of reality.

scholarly journals The synthesis and localization of alternatively spliced fibronectin EIIIB in resting and thrombin-treated megakaryocytes

Blood ◽  
1996 ◽  
Vol 87 (5) ◽  
pp. 1817-1823 ◽  
Author(s):  
PK Schick ◽  
CM Wojenski ◽  
VD Bennett ◽  
T Ivanova
Keyword(s):  
Bone Marrow ◽  
Confocal Microscopy ◽  
Guinea Pig ◽  
Western Blotting ◽  
Bone Marrow Cells ◽  
Cell Maturation ◽  
Metabolic Labeling ◽  
Alternatively Spliced ◽  
Marrow Cells ◽  
Migrating Cells

There are several species of alternatively spliced fibronectin (FN). One of these, FN EIIIB, is primarily present in embryonic and in proliferating and migrating cells and is believed to be important for cell maturation. We have studied the synthesis, localization, and secretion of this FN isoform in isolated guinea pig megakaryocytes, nonmegakaryocytic bone marrow cells, and platelets. There was 7.5 times more general FN in megakaryocytes than in nonmegakaryocytic cells based on the analysis of equivalent amounts of protein. FN EIIIB was detected by Western blotting in megakaryocytes but not in nonmegakaryocytic cells present in bone marrow. Neither megakaryocytes nor platelets secreted FN EIIIB, while megakaryocytes secreted 25.3% +/- 4.6% general FN and platelets secreted about 61% general FN in response to thrombin. Analysis of immunostained cells by confocal microscopy revealed that FN EIIIB had been redistributed to the surface of megakaryocytes in response to thrombin. Synthesis was studied by metabolic labeling, and megakaryocytes were shown to synthesize FN and FN EIIIB. Thus, megakaryocytes and platelets are among a small number of adult cells and tissues that synthesize and contain FN EIIIB. The expression of FN EIIIB on the megakaryocyte surface may influence migration and maturation.

Effect of bone marrow cells on colony-forming guinea pig stromal cells and on proliferation of their descendants in culture

1988 ◽  
Vol 106 (6) ◽  
pp. 1756-1758
Author(s):  
A. I. Zorina ◽  
Yu. F. Gorskaya ◽  
A. G. Grosheva ◽  
A. Ya. Fridenshtein
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Stromal Cells ◽  
Bone Marrow Cells ◽  
Marrow Cells

scholarly journals The synthesis and localization of alternatively spliced fibronectin EIIIB in resting and thrombin-treated megakaryocytes

Blood ◽  
1996 ◽  
Vol 87 (5) ◽  
pp. 1817-1823 ◽  
Author(s):  
PK Schick ◽  
CM Wojenski ◽  
VD Bennett ◽  
T Ivanova
Keyword(s):  
Bone Marrow ◽  
Confocal Microscopy ◽  
Guinea Pig ◽  
Western Blotting ◽  
Bone Marrow Cells ◽  
Cell Maturation ◽  
Metabolic Labeling ◽  
Alternatively Spliced ◽  
Marrow Cells ◽  
Migrating Cells

Abstract There are several species of alternatively spliced fibronectin (FN). One of these, FN EIIIB, is primarily present in embryonic and in proliferating and migrating cells and is believed to be important for cell maturation. We have studied the synthesis, localization, and secretion of this FN isoform in isolated guinea pig megakaryocytes, nonmegakaryocytic bone marrow cells, and platelets. There was 7.5 times more general FN in megakaryocytes than in nonmegakaryocytic cells based on the analysis of equivalent amounts of protein. FN EIIIB was detected by Western blotting in megakaryocytes but not in nonmegakaryocytic cells present in bone marrow. Neither megakaryocytes nor platelets secreted FN EIIIB, while megakaryocytes secreted 25.3% +/- 4.6% general FN and platelets secreted about 61% general FN in response to thrombin. Analysis of immunostained cells by confocal microscopy revealed that FN EIIIB had been redistributed to the surface of megakaryocytes in response to thrombin. Synthesis was studied by metabolic labeling, and megakaryocytes were shown to synthesize FN and FN EIIIB. Thus, megakaryocytes and platelets are among a small number of adult cells and tissues that synthesize and contain FN EIIIB. The expression of FN EIIIB on the megakaryocyte surface may influence migration and maturation.

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