scholarly journals Buoyant density separation of cells. I. The buoyant distribution of guinea pig bone marrow cells.

1975 ◽  
Vol 23 (5) ◽  
pp. 378-389 ◽  
Author(s):  
R C Leif ◽  
S B Smith ◽  
L A Dunlap ◽  
S B Leif
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Bone Marrow Cells ◽  
Buoyant Density ◽  
Cell Types ◽  
Density Fractions ◽  
Density Distributions ◽  
First Approximation ◽  
The Individual ◽  
Marrow Cells

Guinea pig bone marrow cells were separated by buoyant density utilizing linear gradients of bovine serum albumin (BSA). It has finally become possible to characterize the cells present in the density fractions in terms of classical morphology. The development of the Cell Type computer program which calculates the percentages of the individual types of cells present in the fractions and their buoyant density distributions and plots the data has greatly facilitated and improved the accuracy of these studies. Approximately 40 cell types were observed in guinea pig bone marrow. Cells with definitive morphologies such as erythrocytes, the neutrophilic series, the binucleate blast megakaryocyte precursor and cells in mitosis band as virtually single peaks. Cells which are parts of continua or can easily be wrongly classified are found in multiple peaks. The small lymphocytes which are known to be polydisperse are found as five peaks. Because of the very strong benzidine staining by the glutaraldehyde-fixed hemoglobin, some of the erythroblasts were wrongly staged, resulting in a multimodal distribution. The presence of macrocytes further complicated these distributions. The rule that the younger cells are always less dense than the mature cells was adhered to in those cases where the cells could be definitively characterized, such as the neutrophilic series and the blasts. These results indicate that morphology is a good first approximation of reality.

scholarly journals The cultivation of bone marrow cells and cell lines on polymeric films

2011 ◽  
Vol 57 (5) ◽  
pp. 535-543
Author(s):  
M.S. Dolgikh ◽  
D.N. Livak ◽  
M.E. Krasheninnikov ◽  
N.A. Onishchenko
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Covalent Binding ◽  
Cell Types ◽  
Polymeric Films ◽  
Artificial Organ ◽  
Hep G2 ◽  
Different Cell Types ◽  
Cultural Media ◽  
Marrow Cells

The cultivation of multipotent mesenchymal stromal bone marrow cells and cells of A-431, MDCK, Vero, 3T3 and Hep-G2 was performed on polymeric films (PVA) with different hydrophobic fatty acid residues. The cells of different types grew on these films with different intensity, but in the most cases comparable with the cultivation control on usual plastic. The examined films were nontoxic to cells and sufficiently adhesive. They did not changed pH of cultural media, were optically transparent under microscope and comfortable in the experimental work. These films can be used as a model for the artificial organ construction. The covalent binding of different fatty acids to PVA shows possibility of the adaptable changes of films properties (hydrophobity and adhesiveness), and therefore possibility of the creation of optimal conditions for different cell types attachement and growth.

A novel bioaction of PAF: Induction of microbicidal activity in guinea pig bone marrow cells

Lipids ◽  
1988 ◽  
Vol 23 (12) ◽  
pp. 1119-1124 ◽  
Author(s):  
Hidetoshi Hayashi ◽  
Ichiro Kudo ◽  
Toshiyuki Kato ◽  
Ryushi Nozawa ◽  
Shoshichi Nojima ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Bone Marrow Cells ◽  
Microbicidal Activity ◽  
Marrow Cells

scholarly journals Augmented Production of Interleukin-6 by Normal Human Osteoblasts in Response to CD34+ Hematopoietic Bone Marrow Cells In Vitro

Blood ◽  
1997 ◽  
Vol 89 (4) ◽  
pp. 1165-1172 ◽  
Author(s):  
Russell S. Taichman ◽  
Marcelle J. Reilly ◽  
Rama S. Verma ◽  
Stephen G. Emerson
Keyword(s):  
Bone Marrow ◽  
Interleukin 6 ◽  
Transforming Growth Factor ◽  
Bone Marrow Cells ◽  
Hematopoietic Cells ◽  
Cell Types ◽  
Colony Stimulating Factor ◽  
Cd34 Cells ◽  
Stimulating Factor ◽  
Marrow Cells

Abstract Based on anatomic and developmental findings characterizing hematopoietic cells in close approximation with endosteal cells, we have begun an analysis of osteoblast/hematopoietic cell interactions. We explore here the functional interdependence between these two cell types from the standpoint of de novo cytokine secretion. We determined that, over a 96-hour period, CD34+ bone marrow cells had no significant effect on osteoblast secretion of granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, or transforming growth factor-β1 , but in some experiments minor increases in leukemia inhibitory factor levels were observed. However, when CD34+ bone marrow cells were cocultured in direct contact with osteoblasts, a 222% ± 55% (range, 153% to 288%) augmentation in interleukin-6 (IL-6) synthesis was observed. The accumulation of IL-6 protein was most rapid during the initial 24-hour period, accounting for nearly 55% of the total IL-6 produced by osteoblasts in the absence of blood cells and 77% of the total in the presence of the CD34+ cells. Cell-to-cell contact does not appear to be required for this activity, as determined by coculturing the two cell types separated by porous micromembranes. The identity of the soluble activity produced by the CD34+ cells remains unknown, but is not likely due to IL-1β or tumor necrosis factor-α, as determined with neutralizing antibodies. To our knowledge, these data represent the first demonstration that early hematopoietic cells induce the production of molecules required for the function of normal bone marrow microenvironments, in this case through the induction of hematopoietic cytokine (IL-6) secretion by osteoblasts.

Induction by platelet activating factor of candidacidal activity in guinea pig bone marrow cells

1988 ◽  
Vol 10 ◽  
pp. 19
Author(s):  
I. Kudo ◽  
H. Hayashi ◽  
T. Kato ◽  
R. Nozawa ◽  
S. Nojima ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Bone Marrow Cells ◽  
Platelet Activating Factor ◽  
Marrow Cells

scholarly journals CD34 expression by stromal precursors in normal human adult bone marrow

Blood ◽  
1991 ◽  
Vol 78 (11) ◽  
pp. 2848-2853 ◽  
Author(s):  
PJ Simmons ◽  
B Torok-Storb
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Complex Mixture ◽  
Antigen Expression ◽  
Cell Types ◽  
Stromal Precursors ◽  
Cd34 Antigen ◽  
Distinct Cell ◽  
Marrow Cells

Normal bone marrow cells were isolated by fluorescence-activated cell sorting (FACS) on the basis of CD34 antigen expression and then assayed in vitro for colonies of fibroblastic cells (fibroblast colony-forming units [CFU-F]). Greater than 95% of detectable CFU-F were recovered in the CD34+ population, while their numbers were markedly depleted in the CD34- population. Additional experiments showed that the majority of CFU-F exhibited high forward and perpendicular light scatter and low- density CD34 antigen. Growth of sorted cells in medium optimized for long-term marrow culture (LTMC) produced a complex mixture of adherent stromal elements including fibroblasts, adipocytes, smooth muscle cells, and macrophages. Monoclonal antibody STRO-1, which identifies bone marrow stromal cells, reacted with approximately 5% of CD34+ cells, which included all CFU-F and stromal precursors in LTMC. Experiments using soybean agglutinin (SBA) further showed that these stromal elements were restricted to a population of bone marrow cells with the phenotype CD34+/SBA+. These properties of stromal precursors are quite distinct from those of primitive hematopoietic progenitors, showing that although the precursors of the hematopoietic and stromal systems share expression of CD34, they are otherwise phenotypically distinct cell types.

scholarly journals Induction of unscheduled DNA synthesis in human myeloma cells

Blood ◽  
1980 ◽  
Vol 55 (2) ◽  
pp. 311-316
Author(s):  
R Lewensohn ◽  
U Ringborg
Keyword(s):  
Bone Marrow ◽  
Dna Synthesis ◽  
Bone Marrow Cells ◽  
Ultra Violet ◽  
Cell Types ◽  
Unscheduled Dna Synthesis ◽  
Myeloma Cells ◽  
Poorly Differentiated ◽  
The Relationship ◽  
Marrow Cells

Unscheduled DNA synthesis (UDS) induced by melphalan, nitrogen mustard and ultra-violet irradiation was studied in bone marrow cells from myeloma patients. In a previous study, normal bone marrow cells in various stages of maturation were found to display a gradual decrease in UDS parallel with the process of maturation. Myeloma cells showed a similar pattern. Poorly differentiated myeloma cells exhibited a similar level of UDS to myeloblasts and erythroblasts. Irrespective of which repair-inducing agent was used, the relationship between the levels of UDS in the various cell types was constant. This indicates that the differences in the level of UDS in the various cell types was not due to differences in the uptake of the repair-inducing agent.

scholarly journals THE ENHANCING EFFECT OF BONE MARROW CELLS ON THE PRIMARY IMMUNE RESPONSE OF THE ISOLATED PERFUSED SPLEEN

1970 ◽  
Vol 131 (4) ◽  
pp. 833-842 ◽  
Author(s):  
Robert C. Atkins ◽  
William A. Robinson ◽  
Ben Eiseman
Keyword(s):  
Bone Marrow ◽  
Antibody Response ◽  
Bone Marrow Cells ◽  
Cell Types ◽  
Primary Immune Response ◽  
Peripheral Lymphocytes ◽  
Distinct Cell ◽  
Primary Antibody Response ◽  
Marrow Cells

The addition of bone marrow cells or peripheral lymphocytes to the isolated pig spleen markedly enhanced the primary antibody response after 3-day perfusion and antigenic challenge in vitro. The splenic preparation without added cells or with the addition of marrow cells to an irradiated spleen gave a limited response. Contributory evidence is provided that at least two distinct cell types are needed for antibody production. For optimal antibody response by an isolated perfused spleen, marrow cells or peripheral lymphocytes should be added to the system.

scholarly journals CD34 expression by stromal precursors in normal human adult bone marrow

Blood ◽  
1991 ◽  
Vol 78 (11) ◽  
pp. 2848-2853 ◽  
Author(s):  
PJ Simmons ◽  
B Torok-Storb
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Complex Mixture ◽  
Antigen Expression ◽  
Cell Types ◽  
Stromal Precursors ◽  
Cd34 Antigen ◽  
Distinct Cell ◽  
Marrow Cells

Abstract Normal bone marrow cells were isolated by fluorescence-activated cell sorting (FACS) on the basis of CD34 antigen expression and then assayed in vitro for colonies of fibroblastic cells (fibroblast colony-forming units [CFU-F]). Greater than 95% of detectable CFU-F were recovered in the CD34+ population, while their numbers were markedly depleted in the CD34- population. Additional experiments showed that the majority of CFU-F exhibited high forward and perpendicular light scatter and low- density CD34 antigen. Growth of sorted cells in medium optimized for long-term marrow culture (LTMC) produced a complex mixture of adherent stromal elements including fibroblasts, adipocytes, smooth muscle cells, and macrophages. Monoclonal antibody STRO-1, which identifies bone marrow stromal cells, reacted with approximately 5% of CD34+ cells, which included all CFU-F and stromal precursors in LTMC. Experiments using soybean agglutinin (SBA) further showed that these stromal elements were restricted to a population of bone marrow cells with the phenotype CD34+/SBA+. These properties of stromal precursors are quite distinct from those of primitive hematopoietic progenitors, showing that although the precursors of the hematopoietic and stromal systems share expression of CD34, they are otherwise phenotypically distinct cell types.

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