Construction of shuttle expression plasmid and stable expression of foreign gene inmycobacteria andE. Coli

1995 ◽  
Vol 15 (3) ◽  
pp. 138-142 ◽  
Author(s):  
Huangfu Yong-mu ◽  
Zhang Da-jun ◽  
Cheng Ji-zhong ◽  
Qian Min ◽  
Liang Ju-qing ◽  
...  
Keyword(s):  
Stable Expression ◽  
Foreign Gene ◽  
Expression Plasmid

scholarly journals Highly stable expression of a foreign gene from rabies virus vectors.

1996 ◽  
Vol 93 (14) ◽  
pp. 7310-7314 ◽  
Author(s):  
T. Mebatsion ◽  
M. J. Schnell ◽  
J. H. Cox ◽  
S. Finke ◽  
K. K. Conzelmann
Keyword(s):  
Rabies Virus ◽  
Stable Expression ◽  
Foreign Gene ◽  
Virus Vectors

scholarly journals Transfection With Plasmid Causing Stable Expression of a Foreign Gene Affects General Proteome Pattern in Giardia lamblia Trophozoites

2020 ◽  
Vol 10 ◽  
Author(s):  
Manfred Heller ◽  
Sophie Braga ◽  
Norbert Müller ◽  
Joachim Müller
Keyword(s):  
Giardia Lamblia ◽  
Antigenic Variation ◽  
Internal Standard ◽  
Basic Research ◽  
Stable Expression ◽  
Foreign Gene ◽  
Differentially Expressed Proteins ◽  
Transfected Cells ◽  
Genetic Manipulations ◽  
Shotgun Mass Spectrometry

Giardia lamblia is an important causative agent of persistent diarrhea in humans, domestic animals, and cattle. Basic research is usually performed with the strain WBC6 and includes genetic manipulations such as transfections. Here, we investigate how transfection with a plasmid causing stable expression of a foreign gene affects the whole proteome pattern. Using shotgun mass spectrometry, we compare the proteomes of untransfected trophozoites to trophozoites transfected with Escherichia coli glucuronidase A (GusA). Besides GusA, which is detected in the transfected trophozoites only, the proteomes of untransfected and transfected trophozoites differ by 132 differentially expressed proteins. In particular, transfection induces antigenic variation. Since transfection causing stable expression affects the proteome pattern, transfection experiments should take into account this effect. Due to a unique peptide panel, GusA is an example for a suitable internal standard for experiments involving transfected cells. Data are available via ProteomeXchange with identifier PXD022565.

Sign in / Sign up

Export Citation Format

Share Document