scholarly journals Transfection With Plasmid Causing Stable Expression of a Foreign Gene Affects General Proteome Pattern in Giardia lamblia Trophozoites

2020 ◽  
Vol 10 ◽  
Author(s):  
Manfred Heller ◽  
Sophie Braga ◽  
Norbert Müller ◽  
Joachim Müller
Keyword(s):  
Giardia Lamblia ◽  
Antigenic Variation ◽  
Internal Standard ◽  
Basic Research ◽  
Stable Expression ◽  
Foreign Gene ◽  
Differentially Expressed Proteins ◽  
Transfected Cells ◽  
Genetic Manipulations ◽  
Shotgun Mass Spectrometry

Giardia lamblia is an important causative agent of persistent diarrhea in humans, domestic animals, and cattle. Basic research is usually performed with the strain WBC6 and includes genetic manipulations such as transfections. Here, we investigate how transfection with a plasmid causing stable expression of a foreign gene affects the whole proteome pattern. Using shotgun mass spectrometry, we compare the proteomes of untransfected trophozoites to trophozoites transfected with Escherichia coli glucuronidase A (GusA). Besides GusA, which is detected in the transfected trophozoites only, the proteomes of untransfected and transfected trophozoites differ by 132 differentially expressed proteins. In particular, transfection induces antigenic variation. Since transfection causing stable expression affects the proteome pattern, transfection experiments should take into account this effect. Due to a unique peptide panel, GusA is an example for a suitable internal standard for experiments involving transfected cells. Data are available via ProteomeXchange with identifier PXD022565.

scholarly journals Putative SF2 helicases of the early-branching eukaryote Giardia lamblia are involved in antigenic variation and parasite differentiation into cysts

2012 ◽  
Vol 12 (1) ◽  
pp. 284 ◽  
Author(s):  
Pablo R Gargantini ◽  
Marianela C Serradell ◽  
Alessandro Torri ◽  
Hugo D Lujan
Keyword(s):  
Giardia Lamblia ◽  
Antigenic Variation

scholarly journals Comparative proteomics of three Giardia lamblia strains: investigation of antigenic variation in the post-genomic era

Parasitology ◽  
2020 ◽  
Vol 147 (9) ◽  
pp. 1008-1018 ◽  
Author(s):  
Joachim Müller ◽  
Sophie Braga ◽  
Anne-Christine Uldry ◽  
Manfred Heller ◽  
Norbert Müller
Keyword(s):  
Giardia Lamblia ◽  
Antigenic Variation ◽  
Restriction Analysis ◽  
Surface Proteins ◽  
Differentially Expressed ◽  
Laboratory Research ◽  
Persistent Diarrhoea ◽  
Reading Frame ◽  
Expression Levels ◽  
Predominant Variant

AbstractGiardia lamblia is a causative agent of persistent diarrhoea widespread in regions with low hygienic standards. Laboratory research is based on cloned lines issuing from various patient isolates typed in the late 1980s and 90s using restriction analysis and serology. In the present study, we compared the well-characterized strain WBC6 with another clone of the parent WB isolate termed WBA1 and with a clone from another isolate, GS/M-83-H7, using shotgun mass spectrometry proteomics. We identified 398 proteins differentially expressed between the GS and both WB isolates and 97 proteins differentially expressed between the two WB isolates. We investigated the expression levels of the predominant variant-specific surface proteins (VSPs) in each clone and matched the previously described major VSPs of each strain to the corresponding open reading frame sequences identified by whole-genome sequencing efforts. Furthermore, since the original WB isolate comes from a patient treated with metronidazole, we compared the susceptibilities of the strains to nitro compounds, as well the expression levels of enzymes involved in nitro reduction and on the corresponding enzyme activities and found distinct differences between the three strains.

scholarly journals Highly stable expression of a foreign gene from rabies virus vectors.

1996 ◽  
Vol 93 (14) ◽  
pp. 7310-7314 ◽  
Author(s):  
T. Mebatsion ◽  
M. J. Schnell ◽  
J. H. Cox ◽  
S. Finke ◽  
K. K. Conzelmann
Keyword(s):  
Rabies Virus ◽  
Stable Expression ◽  
Foreign Gene ◽  
Virus Vectors

Antigenic variation in Giardia lamblia

1989 ◽  
Vol 68 (2) ◽  
pp. 238-241 ◽  
Author(s):  
Theodore E. Nash
Keyword(s):  
Giardia Lamblia ◽  
Antigenic Variation

scholarly journals A Lesson in Survival, by Giardia lamblia

2010 ◽  
Vol 10 ◽  
pp. 2019-2031 ◽  
Author(s):  
Andrea A. S. Rópolo ◽  
Maria C. Touz
Keyword(s):  
Giardia Lamblia ◽  
Drug Targets ◽  
Antigenic Variation ◽  
Protozoan Parasite ◽  
Arginine Deiminase ◽  
Multiple Roles ◽  
Variation Potential ◽  
Acquired Immune Responses ◽  
The One ◽  
Potential Drug Targets

In the relationships between host and parasites, there is a cross-talk that involves diverse mechanisms developed by two different genetic systems during years of evolution. On the one hand, immunocompetent hosts have developed effective innate and acquired immune responses that are used to restrict or avoid parasitism. On the other hand, parasites evade the immune response, expressing different antigens on their surface or by using other specific mechanisms, such as nutrient depletion. In this review, we analyze the survival mechanisms used by the protozoan parasiteGiardia lambliaduring infection. In particular, we examine the multiple roles played by the enzyme arginine deiminase during colonization of the gut, also involving the parasite's mechanism of antigenic variation. Potential drug targets for the treatment of giardiasis are also discussed.

Construction of shuttle expression plasmid and stable expression of foreign gene inmycobacteria andE. Coli

1995 ◽  
Vol 15 (3) ◽  
pp. 138-142 ◽  
Author(s):  
Huangfu Yong-mu ◽  
Zhang Da-jun ◽  
Cheng Ji-zhong ◽  
Qian Min ◽  
Liang Ju-qing ◽  
...  
Keyword(s):  
Stable Expression ◽  
Foreign Gene ◽  
Expression Plasmid
Sign in / Sign up

Export Citation Format

Share Document