Presence, isolation and characterization of yolk DNA from chicken eggs

1998 ◽  
Vol 41 (3) ◽  
pp. 251-257 ◽  
Author(s):  
Lijun Sun ◽  
Lei Yu ◽  
Chuchu Chen
Keyword(s):  
Isolation And Characterization ◽  
Chicken Eggs ◽  
Yolk Dna

ISOLATION AND CHARACTERIZATION OF INFECTIOUS LARYNGOTRACHEITIS VIRUS IN LAYER CHICKENS

2012 ◽  
Vol 8 (2) ◽  
pp. 123-130 ◽  
Author(s):  
MS Islam ◽  
MSR Khan ◽  
MA Islam ◽  
J Hassan ◽  
S Affroze ◽  
...  
Keyword(s):  
Neutralization Test ◽  
Passive Haemagglutination ◽  
Isolation And Characterization ◽  
Infectious Laryngotracheitis ◽  
Commercial Farms ◽  
Layer Chickens ◽  
Chicken Eggs ◽  
Virus Isolates ◽  
Embryonated Chicken

The present research work was conducted for the isolation and characterization of infectious laryngotracheitis (ILT) virus in layer chickens from commercial farms of Gazipur District. A total of 25 field samples were collected from suspected layer chickens of five commercial farms and were cultivated into 10-12 days old embryonated chicken eggs through chorioallantoic membrane (CAM) route for isolation of field virus. The field viruses were characterized by physico-chemical properties against pH, heat, ether and chloroform, serological test such as virus neutralization test (VNT) and passive haemagglutination (PHA) test and pathogenicity testing. In the embryonated chicken eggs, virus produced discrete pock lesions as early as 2 days of post inoculation and embryo death was recorded within 4-6 days of inoculation. The viruses could be inactivated by pH 4 within 2 hours. Inactivation of viruses was observed at 600C for 6 minutes, 550C for 15 minutes and 380C for 2 days. Ether-chloroform treatment also inactivated the viruses. Virus neutralization test revealed that all the virus isolates were neutralized by antiserum to ILT vaccine. Passive haemagglutination test showed that the tanned sheep RBC sensitized with the virus isolates were agglutinated in presence of the antiserum to ILT vaccine. The pathogenicity test recorded 100% mortality in experimental chickens. Data of this study suggest that the field isolates might be infectious laryngotracheitis virus.DOI = http://dx.doi.org/10.3329/bjvm.v8i2.11194 Bangl. J. Vet. Med. (2010). 8 (2) : 123-130 

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization

Isolation and characterization of complex oligomeric proanthocyanidins from grape: applications of CCC and high field multidimensional NMR methods

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
KB Killday ◽  
JA Glinski ◽  
M Davey ◽  
R Kane ◽  
MS Tempesta
Keyword(s):  
High Field ◽  
Multidimensional Nmr ◽  
Oligomeric Proanthocyanidins ◽  
Isolation And Characterization ◽  
Nmr Methods

Isolation and characterization of an arabinose-specific lectin from the ascomycete Xylaria hypoxylon

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
J Renke ◽  
A Deters ◽  
NS Kumar
Keyword(s):  
Isolation And Characterization

Isolation and characterization of new phenanthrenes from Juncus inflexus and their chemotaxonomic significance

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
B Tóth ◽  
N Kúsz ◽  
A Csorba ◽  
T Kurtán ◽  
J Hohmann ◽  
...  
Keyword(s):  
Isolation And Characterization

Isolation and characterization of acetylcholinesterase inhibitors from Piper longum Linn.

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
Z Khatami ◽  
P Sarkheil ◽  
HR Adhami
Keyword(s):  
Acetylcholinesterase Inhibitors ◽  
Piper Longum ◽  
Isolation And Characterization

Isolation and characterization of a novel O-methyltransferase from the tropical liana Triphyophyllum peltatum

2017 ◽  
Author(s):  
L Passolt ◽  
A Jindaprasert ◽  
T Le Tran ◽  
R Seupel ◽  
G Bringmann ◽  
...  
Keyword(s):  
Isolation And Characterization

Isolation and Characterization of Plasminogen Activator from Pig Leucocytes

1974 ◽  
Vol 31 (01) ◽  
pp. 072-085 ◽  
Author(s):  
M Kopitar ◽  
M Stegnar ◽  
B Accetto ◽  
D Lebez
Keyword(s):  
Molecular Weight ◽  
Plasminogen Activator ◽  
Gel Electrophoresis ◽  
Gel Filtration ◽  
Ph Optimum ◽  
Isolation And Characterization ◽  
Ph Range ◽  
Inhibition Studies ◽  
Final Purification

SummaryPlasminogen activator was isolated from disrupted pig leucocytes by the aid of DEAE chromatography, gel filtration on Sephadex G-100 and final purification on CM cellulose, or by preparative gel electrophoresis.Isolated plasminogen activator corresponds No. 3 band of the starting sample of leucocyte cells (that is composed from 10 gel electrophoretic bands).pH optimum was found to be in pH range 8.0–8.5 and the highest pH stability is between pH range 5.0–8.0.Inhibition studies of isolated plasminogen activator were performed with EACA, AMCHA, PAMBA and Trasylol, using Anson and Astrup method. By Astrup method 100% inhibition was found with EACA and Trasylol and 30% with AMCHA. PAMBA gave 60% inhibition already at concentration 10–3 M/ml. Molecular weight of plasminogen activator was determined by gel filtration on Sephadex G-100. The value obtained from 4 different samples was found to be 28000–30500.

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