Reduced content and abnormal distribution of anionic sites (acid proteoglycans) in the diabetic glomerular basement membrane

1986 ◽  
Vol 51 (1) ◽  
pp. 127-135 ◽  
Author(s):  
R. Rohrbach
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Anionic Sites ◽  
Abnormal Distribution

Abnormal Distribution of Anionic Sites in the Glomerular Basement Membrane in Glomerulonephritis of Dogs Infected with Dirofilaria immitis.

2000 ◽  
Vol 62 (11) ◽  
pp. 1193-1195 ◽  
Author(s):  
Junichi KAMIIE ◽  
Kinji SHIROTA ◽  
Munetaka YAMAKI ◽  
Hitoshi KITAGAWA ◽  
Masahiko WASAKI ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Dirofilaria Immitis ◽  
Anionic Sites ◽  
Abnormal Distribution

scholarly journals Recovery of Anionic Sites of the Glomerular Basement Membrane after Their Disappearance by the Cationic Probe Molecule

Nephron ◽  
1991 ◽  
Vol 59 (3) ◽  
pp. 500-501 ◽  
Author(s):  
Ashio Yoshimura ◽  
Terukuni Ideura ◽  
Kiyoko Nakano ◽  
Hiroaki Oniki ◽  
Yuichi Sugisaki ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Probe Molecule ◽  
Anionic Sites

scholarly journals Recombinant lnterleukin-2 Alone Did Not Induce Proteinuria with Changes in Anionic Sites of the Glomerular Basement Membrane in Rats

Nephron ◽  
1992 ◽  
Vol 61 (1) ◽  
pp. 117-118
Author(s):  
Shouichi Fujimoto ◽  
Yoshitaka Yamamoto ◽  
Shuichi Hisanaga ◽  
Naoto Yokota ◽  
Tanenao Eto
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Anionic Sites

scholarly journals Reduction of heparan sulphate-associated anionic sites in the glomerular basement membrane of rats with streptozotocin-induced diabetic nephropathy

Diabetologia ◽  
1995 ◽  
Vol 38 (10) ◽  
pp. 1169-1175 ◽  
Author(s):  
J. H. M. Berden ◽  
J. van den Born ◽  
A. A. van Kraats ◽  
M. A. H. Bakker ◽  
K. J. M. Assmann ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Heparan Sulphate ◽  
Anionic Sites

Heparan Sulfate–Rich Anionic Sites in the Human Glomerular Basement Membrane

1983 ◽  
Vol 309 (17) ◽  
pp. 1001-1009 ◽  
Author(s):  
Robert L. Vernier ◽  
David J. Klein ◽  
Susan P. Sisson ◽  
John D. Mahan ◽  
Theodore R. Oegema ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Heparan Sulfate ◽  
Glomerular Basement Membrane ◽  
Anionic Sites

Loss of Anionic Sites on the Glomerular Basement Membrane in Transplant Glomerulopathy

Nephron ◽  
1994 ◽  
Vol 66 (2) ◽  
pp. 136-139 ◽  
Author(s):  
S. Chakrabarti ◽  
M. Diocee ◽  
S. Henry ◽  
J. Jeffery ◽  
D. Rush ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Anionic Sites ◽  
Transplant Glomerulopathy

scholarly journals Resolution and limitations of the immunoperoxidase procedure in the localization of extracellular matrix antigens.

1983 ◽  
Vol 31 (7) ◽  
pp. 945-951 ◽  
Author(s):  
P J Courtoy ◽  
D H Picton ◽  
M G Farquhar
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Glomerular Basement Membrane ◽  
Cationized Ferritin ◽  
Anionic Sites ◽  
Double Labeling ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Indirect Immunoperoxidase ◽  
Labeling System

A double labeling system was used to test the resolution of the indirect immunoperoxidase procedure in the localization of extracellular matrix components. A recognizable antigen, cationized ferritin, was first implanted at specific anionic sites (approximately 60 nm periodicity) in the lamina rara interna and externa of the glomerular basement membrane (GBM) and subsequently localized by immunoperoxidase. The coincidence between the location of reaction product and the ferritin clusters was assessed. When the amount of immunoadsorbed peroxidase and time of exposure to the 3,3'-diaminobenzidine (DAB)-containing medium were limited, discrete deposits of reaction product were observed around individual ferritin clusters. When immunolabeling was increased, the whole GBM was stained, and DAB staining was also found along the endothelial plasmalemma and the epithelial plasmalemma at the base of the foot processes at some distance (greater than 100 nm) from the ferritin clusters in the laminae rarae. These findings indicate that oxidized DAB reaction product can diffuse over long distances and be reabsorbed onto cell membranes. Even under limited incubation conditions some diffusion of DAB reaction product was encountered. The value and limitations of the DAB-peroxidase procedures are discussed.

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