PrimersITS1, ITS2 andITS4 detect the intraspecies variability in the internal transcribed spacers and 5.8S rRNA gene region in clinical isolates of fungi

2003 ◽  
Vol 48 (2) ◽  
pp. 233-238 ◽  
Author(s):  
M. Korabečná ◽  
V. Liška ◽  
K. Fajfrlík
Keyword(s):  
Internal Transcribed Spacers ◽  
Clinical Isolates ◽  
Gene Region ◽  
Rrna Gene ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
Intraspecies Variability

Sequence analysis of the ribosomal DNA internal transcribed spacer region in some scallop species (Mollusca: Bivalvia: Pectinidae)

Genome ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 595-604 ◽  
Author(s):  
Ana Insua ◽  
María J López-Piñón ◽  
Ruth Freire ◽  
Josefina Méndez
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Evolutionary Relationship ◽  
Gc Content ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
Aequipecten Opercularis

The internal transcribed spacer (ITS) region of the ribosomal DNA from the European scallops Aequipecten opercularis, Mimachlamys varia, Hinnites distortus, and Pecten maximus was PCR amplified and sequenced. For each species, three or five clones were examined. The size ranged between 636 and 713 bp (ITS1, 209–276 bp; 5.8S rRNA gene, 157 bp; ITS2, 270–294 bp) and GC content ranged between 47 and 50% (ITS1, 43–49%; 5.8S rRNA gene, 56–57%; ITS2, 44–49%). Variation within repeats was minimal; only clones from M. varia and P. maximus displayed a few variable sites in ITS2. Among scallops, including Chlamys farreri whose ITS sequence appears in databases, significant variation was observed in both ITS1 and ITS2. Phylogenetic analysis using ITS1, ITS2, or both spacer sequences always yielded trees with similar topology. Aequipecten opercularis and P. maximus grouped in one clade and the other three scallops (C. farreri, M. varia, and H. distortus) in another, where M. varia and H. distortus are the more closely related species. These results provide new insights into the evolutionary relationships of scallop species and corroborate the close evolutionary relationship between the tribes Aequipectinini and Pectinini previously deduced from 18S rDNA sequences.Key words: scallops, Pectinidae, ribosomal DNA, internal transcribed spacers, phylogeny.

scholarly journals The internal transcribed spacers and 5.8S rRNA gene show extensive diversity among isolates of the species complex

2004 ◽  
Vol 4 (4-5) ◽  
pp. 377-388 ◽  
Author(s):  
M KATSU ◽  
S KIDD ◽  
A ANDO ◽  
M MORETTIBRANCHINI ◽  
Y MIKAMI ◽  
...  
Keyword(s):  
Species Complex ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
5.8S Rrna Gene ◽  
5.8S Rrna

Molecular phylogeny of Pneumocystis based on 5.8S rRNA gene and the internal transcribed spacers of rRNA gene sequences

2008 ◽  
Vol 51 (5) ◽  
pp. 445-452 ◽  
Author(s):  
ZiHui Li ◽  
XianMin Feng ◽  
SiQi Lu ◽  
Fan Zhang ◽  
FengYun Wang ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
Gene Sequences ◽  
5.8S Rrna Gene ◽  
5.8S Rrna

scholarly journals Identification of yeast species from orange fruit and juice by RFLP and sequence analysis of the 5.8S rRNA gene and the two internal transcribed spacers

2003 ◽  
Vol 3 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Francisco Javier Las Heras-Vazquez ◽  
Lydia Mingorance-Cazorla ◽  
Josefa María Clemente-Jimenez ◽  
Felipe Rodriguez-Vico
Keyword(s):  
Sequence Analysis ◽  
Yeast Species ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
Orange Fruit
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