Identification of yeast species from orange fruit and juice by RFLP and sequence analysis of the 5.8S rRNA gene and the two internal transcribed spacers

2003 ◽  
Vol 3 (1) ◽  
pp. 3-9 ◽  
Author(s):  
F LASHERASVAZQUEZ ◽  
L MINGORANCECAZORLA ◽  
J CLEMENTEJIMENEZ ◽  
F RODRIGUEZVICO
Keyword(s):  
Sequence Analysis ◽  
Yeast Species ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
Orange Fruit

scholarly journals Identification of yeast species from orange fruit and juice by RFLP and sequence analysis of the 5.8S rRNA gene and the two internal transcribed spacers

2003 ◽  
Vol 3 (1) ◽  
pp. 3-9 ◽  
Author(s):  
Francisco Javier Las Heras-Vazquez ◽  
Lydia Mingorance-Cazorla ◽  
Josefa María Clemente-Jimenez ◽  
Felipe Rodriguez-Vico
Keyword(s):  
Sequence Analysis ◽  
Yeast Species ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
Orange Fruit

Sequence analysis of the ribosomal DNA internal transcribed spacer region in some scallop species (Mollusca: Bivalvia: Pectinidae)

Genome ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 595-604 ◽  
Author(s):  
Ana Insua ◽  
María J López-Piñón ◽  
Ruth Freire ◽  
Josefina Méndez
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Evolutionary Relationship ◽  
Gc Content ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
Aequipecten Opercularis

The internal transcribed spacer (ITS) region of the ribosomal DNA from the European scallops Aequipecten opercularis, Mimachlamys varia, Hinnites distortus, and Pecten maximus was PCR amplified and sequenced. For each species, three or five clones were examined. The size ranged between 636 and 713 bp (ITS1, 209–276 bp; 5.8S rRNA gene, 157 bp; ITS2, 270–294 bp) and GC content ranged between 47 and 50% (ITS1, 43–49%; 5.8S rRNA gene, 56–57%; ITS2, 44–49%). Variation within repeats was minimal; only clones from M. varia and P. maximus displayed a few variable sites in ITS2. Among scallops, including Chlamys farreri whose ITS sequence appears in databases, significant variation was observed in both ITS1 and ITS2. Phylogenetic analysis using ITS1, ITS2, or both spacer sequences always yielded trees with similar topology. Aequipecten opercularis and P. maximus grouped in one clade and the other three scallops (C. farreri, M. varia, and H. distortus) in another, where M. varia and H. distortus are the more closely related species. These results provide new insights into the evolutionary relationships of scallop species and corroborate the close evolutionary relationship between the tribes Aequipectinini and Pectinini previously deduced from 18S rDNA sequences.Key words: scallops, Pectinidae, ribosomal DNA, internal transcribed spacers, phylogeny.

scholarly journals Hagleromyces gen. nov., a yeast genus in the Saccharomycetaceae, and description of Hagleromyces aurorensis sp. nov., isolated from water tanks of bromeliads

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2915-2918 ◽  
Author(s):  
Francisca M. P. Sousa ◽  
Paula B. Morais ◽  
Marc-André Lachance ◽  
Carlos A. Rosa
Keyword(s):  
Rna Polymerase Ii ◽  
Yeast Species ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
Ascomycetous Yeast ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
Lsu Rrna Gene ◽  
Water Tanks

Three strains of a novel yeast species were isolated from water tanks (phytotelmata) of a bromeliad species collected in the state of Tocantins, Brazil. Analysis of sequences for the region spanning the SSU rRNA gene, the internal transcribed spacer, the 5.8S rRNA gene and the D1/D2 domains of the LSU rRNA gene and RNA polymerase II gene showed that these novel yeasts belong to a species that is distinct from all recognized ascomycetous yeast species. Based on the results of gene sequence analyses, a novel species representing a new genus in the Saccharomycetaceae is proposed. The novel species is assigned to the genus Hagleromyces gen. nov. The three isolates of the novel yeast species failed to form sexual spores alone or in mixtures. The name Hagleromyces aurorensis sp. nov. is proposed to accommodate these isolates. The type strain of H. aurorensis sp. nov. is UFMG-CM-Y311T ( = CBS 13264T).

scholarly journals The internal transcribed spacers and 5.8S rRNA gene show extensive diversity among isolates of the species complex

2004 ◽  
Vol 4 (4-5) ◽  
pp. 377-388 ◽  
Author(s):  
M KATSU ◽  
S KIDD ◽  
A ANDO ◽  
M MORETTIBRANCHINI ◽  
Y MIKAMI ◽  
...  
Keyword(s):  
Species Complex ◽  
Internal Transcribed Spacers ◽  
Rrna Gene ◽  
5.8S Rrna Gene ◽  
5.8S Rrna

scholarly journals Comparison of the 5.8S rRNA Gene and Internal Transcribed Spacer Regions of Trichomonadid Protozoa Recovered from the Bovine Preputial Cavity

2003 ◽  
Vol 15 (1) ◽  
pp. 14-20 ◽  
Author(s):  
R. L. Walker ◽  
D. C. Hayes ◽  
S. J. Sawyer ◽  
R. W. Nordhausen ◽  
K. A. Van Hoosear ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Internal Transcribed Spacer ◽  
Rrna Gene ◽  
Tritrichomonas Foetus ◽  
Similarity Matrix ◽  
Multiple Sequence ◽  
Internal Transcribed Spacer Regions ◽  
5.8S Rrna Gene ◽  
5.8S Rrna ◽  
High Degree

Sequence analysis of the 5.8S rRNA gene and the internal transcribed spacer regions (ITSRs) was used to compare trichomonadid protozoa ( n = 39) of varying morphologies isolated from the bovine preputial cavity. A multiple sequence alignment was performed with bovine isolate sequences and other trichomonadid protozoa sequences available in GenBank. As a group, Tritrichomonas foetus isolates ( n = 7) had nearly complete homology. A similarity matrix showed low homology between the T. foetus isolates and other trichomonads recovered from cattle (<70%). Two clusters of trichomonads other than T. foetus were identified. Eighteen isolates comprised 1 group. These isolates shared >99% homology among themselves and with Pen-tatrichomonas hominis. The other non– T. foetus cluster ( n = 14) did not exhibit a high degree of homology (<87%) with other bovine isolates or any of the trichomonad sequences available in GenBank. The sequence homology among isolates in that cluster was >99%, except for 1 isolate that varied from the others in both ITSRs (∼2% dissimilarity). Sequence analysis of the 5.8S rRNA gene and ITSRs was useful for comparing trichomonadid protozoa isolated from the bovine preputial cavity and demonstrated that 2 distinct types of trichomonads constituted the non– T. foetus isolates recovered from the bovine preputial cavity.

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