Fast protein staining in sodium dodecyl sulfate polyacrylamide gel using counter ion-dyes, coomassie brilliant blue R-250 and neutral red

2002 ◽  
Vol 25 (5) ◽  
pp. 704-708 ◽  
Author(s):  
Jung-Kap Choi ◽  
Gyurng-Soo Yoo
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Neutral Red ◽  
Polyacrylamide Gel ◽  
Brilliant Blue ◽  
Coomassie Brilliant Blue ◽  
Counter Ion ◽  
Dodecyl Sulfate ◽  
Protein Staining ◽  
Coomassie Brilliant

Improved measurement of urinary total protein (including light-chain proteins) with a Coomassie brilliant blue G-250-sodium dodecyl sulfate reagent.

1987 ◽  
Vol 33 (11) ◽  
pp. 2100-2106 ◽  
Author(s):  
D K Wimsatt ◽  
J A Lott
Keyword(s):  
Multiple Myeloma ◽  
Sodium Dodecyl Sulfate ◽  
Light Chain ◽  
Sodium Dodecyl ◽  
Brilliant Blue ◽  
Urinary Proteins ◽  
Brilliant Blue G ◽  
Coomassie Brilliant Blue ◽  
Dodecyl Sulfate ◽  
Coomassie Brilliant

Abstract The Coomassie Brilliant Blue G-250 method for urinary proteins underestimates urinary immunoglobulin light chains when albumin or pooled serum is used as the protein standard. The specific color yields of these and other proteins can be brought closer together by adding sodium dodecyl sulfate to the reagent; however, there is some loss of sensitivity. We found such a reagent to be satisfactory for assaying urinary proteins on studying 43 patients with light-chain proteinuria, 19 of whom had multiple myeloma and six possible multiple myeloma.

Variations of Staining Sodium Dodecyl Sulfate–Polyacrylamide Gels with Coomassie Brilliant Blue

2021 ◽  
Vol 2021 (12) ◽  
pp. pdb.prot102236 ◽  
Author(s):  
Clara L. Kielkopf ◽  
William Bauer ◽  
Ina L. Urbatsch
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Standard Procedure ◽  
Sodium Dodecyl ◽  
Brilliant Blue ◽  
Blue Staining ◽  
Staining Procedure ◽  
Polyacrylamide Gels ◽  
Coomassie Brilliant Blue ◽  
Coomassie Brilliant Blue Staining ◽  
Coomassie Brilliant

Many variations of the original Coomassie Brilliant Blue staining procedure are in use. This protocol describes some selected variations on the standard procedure that give comparable and consistent staining results for proteins in the 20- to 200-kDa range.

Sign in / Sign up

Export Citation Format

Share Document