Fulminant Danon Disease in a Young Female Patient with a Novel LAMP2 Mutation

A 14-year-old female presented for congestive heart failure with biventricular hypertrophy and severely depressed LVEF. Cardiac MRI findings of extensive LGE but sparing the mid septum, genetic tests reporting a LAMP2 missense mutation and LAMP2 protein staining in the minority of cardiac myocytesat immunohistochemistry suggested a Danon Disease diagnosis.

A rapid method for protein staining in polyacrylamide gels using water saturated with chloroform

Polyacrylamide gel electrophoresis, followed by an appropriate staining, is a popular and useful analytical procedure for protein identification and characterization. The aim of this study was to develop a method for protein visualization in polyacrylamide gels that would be alternative to Coomassie blue or silver staining. The proposed method is simple, fast and inexpensive. The optimized protocol for protein staining and visualization takes as little as 6 minutes and utilizes deionized water and chloroform. Fluorescence of proteins is induced by UV light and can be detected with a standard transilluminator.

Protein Staining and Analysis with Artificial Intelligence Color Algorithm Analysis with 2-Dimensional Inkjet Printer to Identify Covid-19 Adhesion and Infected Properties on Artificial Body Fluid

In this study, the predictive modeling of Covid-19 viral contamination on artificial body fluids (simulated lung fluid, perspiration, saliva, and urine) was done by colorimetric analysis of protein staining on paper ground with henna ethanolic extract loaded on a 2-dimensional printer cartridge. The purchased artificial body fluids were left in the open-air atmosphere of sterile containers to the public places of pandemic hospitals, pharmacies, which are the most frequent places of Covid-19 patients for 2 weeks. These body fluids, which were then thought to be Covid-19 adsorption, were safely collected and placed in the cartridge chambers of the 2-D printer. The mixtures of artificial body fluids and henna plant extract in the cartridges were sprayed on the paper floor by ink painting. The algorithmic matrix code system was created by colorimetric predictive scaling between +1 and -1 about the images of viral transmission to artificial body fluids taken with a smartphone.