Synthesis of New Hybrid Structured Magnetite Crosslinked Poly Ionic Liquid for Efficient Removal of Coomassie Brilliant Blue R-250 Dye in Aqueous Medium

In this work, new crosslinked pyridinium poly ionic liquid and its magnetite hybrid structured composite were prepared and applied to remove the toxic dye Coomassie Brilliant Blue (CBB-R250) from aqueous solutions. In this respect, vinyl pyridine, maleic anhydride, and dibromo nonane were used to prepare crosslinked quaternized vinyl pyridinium/maleic anhydride ionic liquid (CQVP-MA). Furthermore, a linear copolymer was prepared by the reaction of vinyl pyridine with bromo nonane followed by its copolymerization with maleic anhydride in order to use it as a capping agent for magnetite nanoparticles. The monodisperse MNPs were incorporated into the crosslinked PIL (CQVP-MA) by ultrasonication to prepare CQVP-MA/Fe3O4 composite to facilitate its recovery using an external magnetic field and enhance its adsorption capacity. The chemical structures, thermal stabilities, zeta potential, particle size, EDS, and SEM of the prepared CQVP-MA and CQVP-MA/Fe3O4 were investigated. Adsorption kinetics, isotherms, and mechanisms of CB-R250 elimination from aqueous solutions using CQVP-MA and CQVP-MA/Fe3O4 were also studied, and the results revealed that the pseudo second-order kinetic model and the Langmuir isotherm model were the most suitable to describe the CBB adsorption from an aqueous solution. The adsorption capacities of CQVP-MA and CQVP-MA/Fe3O4 were found to be 1040 and 1198, respectively, which are more than those for previously reported material in the literature with reasonable stability for five cycles.

Variations of Staining Sodium Dodecyl Sulfate–Polyacrylamide Gels with Coomassie Brilliant Blue

Many variations of the original Coomassie Brilliant Blue staining procedure are in use. This protocol describes some selected variations on the standard procedure that give comparable and consistent staining results for proteins in the 20- to 200-kDa range.

Green synthesis of silver nanoparticles by Exiguobacterium profundum isolated from carpet industry effluent

Green synthesis of silver nanoparticles using microorganisms has received profound interest because of their potential to synthesize nanoparticles of various size, shape and morphology. The main objective of the current study was to evaluate the synthesis of silver nanoparticles by a bacterial strain, Exiguobacterium profundum with potential dye degrading ability isolated from carpet industry effluent. The strain was tested for its ability to degrade various dyes including Methylene Blue, Malachite Green, Congo Red, Crystal Violet, Coomassie Brilliant Blue, Methyl Red, Sudan Black. Treatment of the bacteria with 1 mM AgNO3, showed its ability to form silver nanoparticles. This was confirmed by visual observation and UV–Vis absorption at 450 nm. Further characterization of nanoparticles by Scanning electron microscopy confirmed the size of silver nanoparticles in 20-40 nm range. Therefore, the present study is a demonstration of an efficient synthesis of stable silver nanoparticles using Exiguobacterium profundum which could be utilized in dye degradation thereby reducing the pollution from industrial effluents.

Coomassie brilliant blue G 250 modified carbon paste electrode sensor for the voltammetric detection of dihydroxybenzene isomers

In this present study, coomassie brilliant blue G-250 (CBBG) modified electrode was fabricated for the specific and simultaneous detection of three dihydroxybenzene isomers such as resorcinol (RS), catechol (CC) and hydroquinone (HQ). The fabrication of the modified electrode was carried out by electrochemical polymerization of CBBG on the surface of unmodified electrode. The surface structures of bare and fabricated electrode were studied by scanning electron microscope (SEM). The established electrode portrays the very fine interface with these isomers and displayed the sufficient sensitivity and selectivity. The specific parameters of pH solution, scan rate and varying the concentration of analytes were optimized at the modified electrode. The sensor process was originated to be adsorption-controlled activity and the low limit of detection (LOD) for RS and CC was attained at 0.24 and 0.21 µM respectively. In the simultaneous study, designed sensor clearly implies the three well separated anodic peaks for RS, HQ and CC nevertheless in unmodified electrode it failed. Also, the constructed electrode was applied for the real sample analysis in tap water and obtained results are agreeable and it consistent in-between 92.80–99.48%.