Identification and Characterization of Bean yellow mosaic virus Infecting Freesia

2009 ◽  
Vol 18 (2) ◽  
pp. 253-255 ◽  
Author(s):  
Yogesh Kumar ◽  
Vipin Hallan ◽  
Aijaz A. Zaidi
2002 ◽  
Vol 68 (4) ◽  
pp. 378-381 ◽  
Author(s):  
Hiroyuki UGA ◽  
Yuki O. KOBAYASHI ◽  
Kyoji HAGIWARA ◽  
Yohachiro HONDA ◽  
Toshihiro OMURA ◽  
...  

VirusDisease ◽  
2015 ◽  
Vol 26 (4) ◽  
pp. 315-318 ◽  
Author(s):  
P. N. Sharma ◽  
Vivek Sharma ◽  
Anuradha Sharma ◽  
Kajal Rajput ◽  
S. K. Sharma

Author(s):  
T.C. Archith ◽  
V. Devappa ◽  
B. Manjunath ◽  
Chirag Reddy

Background: French bean (Phaseolus vulgaris L.), is one of the important legume vegetables grown in India. The disease mung bean yellow mosaic virus (MYMV) limits successful production of beans. MYMV has become an epidemic in bean growing regions, particularly in the tropical and subtropical countries. In summer, the disease is more serious and widely distributed and reported in various countries. The current study is aimed at identification of MYMV and molecular characterization of mung bean yellow mosaic virus in French bean. This study will be helpful for early detection of the virus, so that better preventive measures can be taken to control MYMV.Methods: The investigations were conducted during 2016-2017, all the agro climatic zones of Karnataka province were surveyed. The coat protein (CP) gene of MYMV was amplified using gene specific primer with DNA isolated from MYMV infected leaves samples in french bean. Polymerase chain reaction products were Sequenced and analysed using a bioinformatic tool. Result: The CP gene decrypt sequences analysis revealed that the identity and similarity in global alignment for all the obtained sequences ranging from 80.8 to 95.3% with reference to MYMV, whereas for horse gram yellow mosaic virus (HYMV) it ranges from 90.4 to 99.1%. Two distinct yellow mosaic viruses infecting french bean (MYMV and HYMV) were identified and it was observed that there exists considerable genetic variation among these species. Present work showed that the CP region is efficient enough to provide a simple, rapid and reliable method for early detection of MYMV infections in french bean, which would help to develop proper management strategies to control these viruses.


Author(s):  
P. Hemachandra Reddy ◽  
V. Venkataravanappa ◽  
P. Swarnalatha ◽  
K. V. Ashwathappa ◽  
M. Krishna Reddy

1968 ◽  
Vol 38 (2) ◽  
pp. 316-328 ◽  
Author(s):  
M. Weintraub ◽  
H. W. J. Ragetli

The composition of crystalline inclusions and dense bands in cells of broad bean leaves infected with bean yellow mosaic virus was studied by differential enzymatic digestion. Frozen thick sections were prefixed in glutaraldehyde and exposed to proteinases and nucleases, after which ultrathin sections were prepared for electron microscopy. Examination revealed that the crystals were completely digested by pepsin in 30 min, whereas the dense bands remained intact for the first 20 min, and could not be found after longer periods of digestion. When ultrathin sections of tissues embedded in glycol methacrylate were incubated with the enzymes, pepsin digested the crystals; this left only a filamentous residue which did not disappear after further exposure to pepsin or to RNase. Trypsin had the same effect, but was slower and less consistent. The dense bands were entirely digested in thin sections by pepsin and trypsin. Neither inclusion was affected by RNase or DNase in thick or thin sections. These results demonstrate that the crystals and dense bands are composed entirely or primarily of protein, but there is no evidence that they contain nucleic acid.


1996 ◽  
Vol 62 (5) ◽  
pp. 472-477 ◽  
Author(s):  
Shigeo NAKAMURA ◽  
Ryoso HONKURA ◽  
Takayoshi IWAI ◽  
Masashi UGAKI ◽  
Yuko OHASHI

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