Isolation and characterization of the fission yeast gene rpa42 +, which encodes a subunit shared by RNA polymerases I and III

1999 ◽  
Vol 262 (4-5) ◽  
pp. 749-757 ◽  
Author(s):  
Y. Imazawa ◽  
K. Imai ◽  
A. Fukushima ◽  
K. Hisatake ◽  
M. Muramatsu ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Rna Polymerases ◽  
Yeast Gene ◽  
Isolation And Characterization ◽  
A Subunit

Isolation and characterization of the fission yeast gene

1999 ◽  
Vol 262 (4) ◽  
pp. 749 ◽  
Author(s):  
Y. Imazawa ◽  
K. Imai ◽  
A. Fukushima ◽  
K. Hisatake ◽  
M. Muramatsu ◽  
...  
Keyword(s):  
Fission Yeast ◽  
Yeast Gene ◽  
Isolation And Characterization

Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

Isolation and characterization of mutant bacteriophage T7 RNA polymerases

1992 ◽  
Vol 224 (2) ◽  
pp. 307-318 ◽  
Author(s):  
Debabrata Patra ◽  
Eileen M. Lafer ◽  
Rui Sousa
Keyword(s):  
Rna Polymerases ◽  
Bacteriophage T7 ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of a precursor form of the ‘A’ subunit of cholera toxin

FEBS Letters ◽  
1981 ◽  
Vol 126 (2) ◽  
pp. 187-190 ◽  
Author(s):  
Lawrence K. Duffy ◽  
Johnny W. Peterson ◽  
Alexander Kurosky
Keyword(s):  
Cholera Toxin ◽  
Isolation And Characterization ◽  
A Subunit
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