Chromosomal location of the crown rust resistance gene Pc98 in cultivated oat (Avena sativa L.)

Segregating F3 families, derived from a cross between oat cultivar Swan and the putative single gene line PC68, were used to determine the association of seed storage protein loci and resistance gene analogues (RGAs) with the crown rust resistance gene Pc68. SDS–PAGE analysis detected three avenin loci, AveX, AveY, and AveZ, closely linked to Pc68. Their diagnostic alleles are linked in coupling to Pc68 and were also detected in three additional lines carrying Pc68. Another protein locus was linked in repulsion to Pc68. In complementary studies, three wheat RGA clones (W2, W4, and W10) detected restriction fragment length polymorphisms (RFLPs) between homozygous resistant and homozygous susceptible F3 DNA bulks. Four oat homologues of W2 were cloned and sequenced. RFLPs detected with two of them were mapped using F3 and F4 populations. Clone 18 detected a locus, Orga2, linked in repulsion to Pc68. Clone 22 detected several RFLPs including Orga1 (the closest locus to Pc68) and three RGA loci (Orga22-2, Orga22-3, and Orga22-4) loosely linked to Pc68. The diagnostic RFLPs linked in coupling to Pc68 were detected by clone 22 in three additional oat lines carrying Pc68 and have potential utility in investigating and improving crown rust resistance of oat.

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Mapping Oat Crown Rust Resistance Gene Pc45 Confirms Association with PcKM

G3 Genes|Genome|Genetics ◽

10.1534/g3.118.200757 ◽

2018 ◽

pp. g3.200757.2018 ◽

Cited By ~ 5

Author(s):

Aida Z. Kebede ◽

Jayelle R. Friesen-Enns ◽

Belaghihalli N. Gnanesh ◽

Jim G. Menzies ◽

Jennifer W. Mitchell Fetch ◽

...

Keyword(s):

Resistance Gene ◽

Rust Resistance ◽

Crown Rust ◽

Rust Resistance Gene ◽

Oat Crown Rust ◽

Crown Rust Resistance

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Dosage Dependent Genetic Suppression of Oat Crown Rust Resistance Gene Pc‐62

Crop Science ◽

10.2135/cropsci1997.0011183x003700060004x ◽

1997 ◽

Vol 37 (6) ◽

pp. 1699-1705 ◽

Cited By ~ 17

Author(s):

W. A. Wilson ◽

M. S. McMullen

Keyword(s):

Resistance Gene ◽

Rust Resistance ◽

Crown Rust ◽

Rust Resistance Gene ◽

Oat Crown Rust ◽

Crown Rust Resistance ◽

Genetic Suppression

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Genetic analysis and molecular mapping of a seedling crown rust resistance gene in oat

Theoretical and Applied Genetics ◽

10.1007/s00122-014-2425-5 ◽

2014 ◽

Vol 128 (2) ◽

pp. 247-258 ◽

Cited By ~ 11

Author(s):

Belaghihalli N. Gnanesh ◽

Curt A. McCartney ◽

Peter E. Eckstein ◽

Jennifer W. Mitchell Fetch ◽

Jim G. Menzies ◽

...

Keyword(s):

Genetic Analysis ◽

Resistance Gene ◽

Rust Resistance ◽

Molecular Mapping ◽

Crown Rust ◽

Rust Resistance Gene ◽

Crown Rust Resistance

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Molecular Mapping of the Crown Rust Resistance Gene Rpc1 in Barley

Phytopathology ◽

10.1094/phyto.2004.94.8.858 ◽

2004 ◽

Vol 94 (8) ◽

pp. 858-861 ◽

Cited By ~ 5

Author(s):

H. A. Agrama ◽

L. Dahleen ◽

M. Wentz ◽

Y. Jin ◽

B. Steffenson

Keyword(s):

Resistance Gene ◽

Ssr Markers ◽

Rust Resistance ◽

Molecular Mapping ◽

Rapd Marker ◽

The United States ◽

Crown Rust ◽

Rust Resistance Gene ◽

Rflp Markers ◽

Crown Rust Resistance

Crown rust of barley, caused by Puccinia coronata var. hordei, occurs sporadically and sometimes may cause yield and quality reductions in the Great Plains region of the United States and Canada. The incompletely dominant resistance allele Rpc1 confers resistance to P. coronata in barley. Two generations, F2 and F2:3, developed from a cross between the resistant line Hor2596 (CIho 1243) and the susceptible line Bowman (PI 483237), were used in this study. Bulked segregant analysis combined with random amplified polymorphic DNA (RAPD) primers were used to identify molecular markers linked to Rpc1. DNA genotypes produced by 500 RAPD primers, 200 microsatellites (SSRs), and 71 restriction fragment length polymorphism (RFLP) probes were applied to map Rpc1. Of these, 15 RAPD primers identified polymorphisms between resistant and susceptible bulks, and 62 SSR markers and 32 RFLP markers identified polymorphisms between the resistant and susceptible parents. The polymorphic markers were applied to 97 F2 individuals and F2:3 families. These markers identified 112 polymorphisms and were used for primary linkage mapping to Rpc1 using Map Manager QT. Two RFLP and five SSR markers spanning the centromere on chromosome 3H and one RAPD marker (OPO08-700) were linked with Rpc1 and, thus, used to construct a 30-centimorgan (cM) linkage map containing the Rpc1 locus. The genetic distance between Rpc1 and the closest marker, RAPD OPO08-700, was 2.5 cM. The linked markers will be useful for incorporating this crown rust resistance gene into barley breeding lines.

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