Alien introgression and morpho-agronomic characterization of diploid progenies of Solanum lycopersicoides monosomic alien addition lines (MAALs) toward pre-breeding applications in tomato (S. lycopersicum)

Abstract Key message Alien introgressions that were captured in the genome of diploid plants segregating from progenies of monosomic alien addition lines of S. lycopersicoides confer novel phenotypes with commercial and agronomic value in tomato breeding. Abstract Solanum lycopersicoides is a wild relative of tomato with a natural adaptation to a wide array of biotic and abiotic challenges. In this study, we identified and characterized diploid plants segregating from the progenies of monosomic alien addition lines (MAALs) of S. lycopersicoides to establish their potential as donors in breeding for target trait improvement in tomato. Molecular genotyping identified 28 of 38 MAAL progenies having the complete chromosome complement of the cultivated tomato parent and limited chromosome introgressions from the wild S. lycopersicoides parent. Analysis of SSR and indel marker profiles identified 34 unique alien introgressions in the 28 MAAL-derived introgression lines (MDILs) in the genetic background of tomato. Conserved patterns of alien introgressions were detected among sibs of MDILs 2, 3, 4 and 8. Across MDILs, a degree of preferential transmission of specific chromosome segments was also observed. Morphologically, the MDILs closely resembled the cultivated tomato more than S. lycopersicoides. The appearance of novel phenotypes in the MDILs that are lacking in the cultivated parent or the source MAALs indicates the capture of novel genetic variation by the diploid introgression lines that can add commercial and agronomic value to tomato. In particular, screening of representative MDILs for drought tolerance at the vegetative stage identified MDIL 2 and MDIL 11III as drought tolerant based on visual scoring. A regulated increase in stomatal conductance of MDIL 2 under drought stress indicates better water use efficiency that allowed it to survive for 7 days under 0% moisture level.

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A Solanum lycopersicoides reference genome facilitates biological discovery in tomato

10.1101/2020.04.16.039636 ◽

2020 ◽

Author(s):

Adrian F. Powell ◽

Lance E. Courtney ◽

Maximilian H.-W. Schmidt ◽

Ari Feder ◽

Alexander Vogel ◽

...

Keyword(s):

Pathogen Resistance ◽

Wild Relatives ◽

Introgression Lines ◽

Tomato Genome ◽

Nutrient Quality ◽

Solanum Lycopersicoides ◽

Genomic Studies ◽

Biological Discovery ◽

Cultivated Species ◽

Tomato Breeding

SummaryWild relatives of tomato are a valuable source of natural variation in tomato breeding, as many can be hybridized to the cultivated species (Solanum lycopersicum). Several, including Solanum lycopersicoides, have been crossed to S. lycopersicum for the development of ordered introgression lines (ILs). Despite the utility of these wild relatives and their associated ILs, limited finished genomes have been produced to aid genetic and genomic studies. We have generated a chromosome-scale genome assembly for Solanum lycopersicoides LA2951 using PacBio sequencing, Illumina, and Hi-C. We identified 37,938 genes based on Illumina and Isoseq and compared gene function to the available cultivated tomato genome resources, in addition to mapping the boundaries of the S. lycopersicoides introgressions in a set of cv. VF36 x LA2951 introgression lines (IL). The genome sequence and IL map will support the development of S. lycopersicoides as a model for studying fruit nutrient/quality, pathogen resistance, and environmental stress tolerance traits that we have identified in the IL population and are known to exist in S. lycopersicoides.

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A library of Solanum lycopersicoides introgression lines in cultivated tomato

Genome ◽

10.1139/g05-032 ◽

2005 ◽

Vol 48 (4) ◽

pp. 685-697 ◽

Cited By ~ 74

Author(s):

Michael A Canady ◽

Vladimir Meglic ◽

Roger T Chetelat

Keyword(s):

Lycopersicon Esculentum ◽

Segregation Distortion ◽

Germ Line ◽

Introgression Lines ◽

Primary Group ◽

Solanum Lycopersicoides ◽

Minimum Number ◽

Map Resolution ◽

Strong Segregation ◽

Chromosome Segments

A set of introgression lines (ILs), containing individual chromosome segments from the wild nightshade Solanum lycopersicoides bred into the genetic background of cultivated tomato (Lycopersicon esculentum), has been developed. A primary group of 56 lines was selected for maximum representation of the S. lycopersicoides genome (~96% of the total map units), homozygosity, and a minimum number of introgressed segments per line. A secondary set of 34 lines provides increased map resolution in certain regions. Approximately 34% of the lines were sterile in the homozygous condition, but could be maintained by heterozygotes. To facilitate identification of segregating ILs, restriction fragment length polymorphism probes were converted to higher throughput cleaved amplified polymorphic sequence markers, which supplement allozyme and morphological loci. Strong segregation distortion was observed in F2 progeny of heterozygous ILs, with an excess of L. esculentum alleles in most regions. For introgressions on distal chromosome 1L, a preferential transmission of S. lycopersicoides alleles was observed in the male germ line. Homozygous ILs generally yielded less seed from self pollination than corresponding heterozygotes, indicating that sterility effects were recessive. This IL library provides a novel resource for genetic studies of traits found in S. lycopersicoides.Key words: Lycopersicon esculentum, Solanum lycopersicum, Solanum lycopersicoides, segregation distortion, alien introgression.

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Identification, transmission, and cytological behavior of Solanum lycopersicoides Dun. monosomic alien addition lines in tomato (Lycopersicon esculentum Mill.)

Genome ◽

10.1139/g97-095 ◽

1998 ◽

Vol 41 (1) ◽

pp. 40-50 ◽

Cited By ~ 24

Author(s):

Roger T Chetelat ◽

Charles M Rick ◽

Pedro Cisneros ◽

Kevin B Alpert ◽

Joseph W DeVerna

Keyword(s):

Lycopersicon Esculentum ◽

Extra Chromosome ◽

Addition Lines ◽

Solanum Lycopersicoides ◽

Visual Markers ◽

Total Progeny ◽

Fruit Characters ◽

Sexual Crosses ◽

Homoeologous Chromosomes ◽

Alien Addition Lines

The wild nightshade Solanum lycopersicoides possesses a number of disease- and arthropod-resistance and environmental stress tolerance traits of potential value for the cultivated tomato, Lycopersicon esculentum. F1 hybrids are readily obtained by sexual crosses, but backcrosses to tomato are hindered by sterility and incompatibility barriers. In the present studies, intergeneric sesquidiploid hybrids (2 genomes of L. esculentum and 1 of S. lycopersicoides) were pollinated with Lycopersicon pennellii derived bridging lines, yielding diploid, aneuploid (up to 2n + 6), and polyploid (3x, 4x) progeny. After diploids (58% of total progeny), the group most frequently obtained was 2n + 1 (31%), which included all 12 possible monosomic alien addition lines (MAALs) containing a single extra chromosome from S. lycopersicoides in a tomato background. MAAL groups were identified with a combination of allozyme, RFLP, and monogenic visual markers. Transmission rates of the extra chromosomes in MAALs varied from 0%, for chromosome 6, to 24%, for chromosome 10. At diakinesis and metaphase I of meiosis, the alien chromosome was usually unpaired (in an average of 90% of PMCs), but association of homoeologous chromosomes was observed in each of the 12 MAAL groups (in an average of 5.7% of PMCs). The MAALs showed a striking morphological resemblance to the corresponding tomato primary trisomic. In addition, each MAAL expressed S. lycopersicoides specific leaf, flower, and (or) fruit characters. This set of MAALs, the first of its kind in Lycopersicon, is expected to be useful for a variety of gene-mapping and introgression studies.

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Molecular and phenotypic characterization of doubled haploid exotic introgression lines for nitrogen use efficiency in maize

10.31274/etd-180810-5033 ◽

2017 ◽

Author(s):

Darlene Lonjas Sanchez

Keyword(s):

Nitrogen Use Efficiency ◽

Doubled Haploid ◽

Introgression Lines ◽

Phenotypic Characterization ◽

Nitrogen Use ◽

Use Efficiency

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Generation and characterization of Brassica rapa ssp. pekinensis – B. oleracea var. capitata monosomic and disomic alien addition lines

Journal of Genetics ◽

10.1007/s12041-015-0542-9 ◽

2015 ◽

Vol 94 (3) ◽

pp. 435-444 ◽

Cited By ~ 2

Author(s):

AI XIA GU ◽

SHU XING SHEN ◽

YAN HUA WANG ◽

JIAN JUN ZHAO ◽

SHU XIN XUAN ◽

...

Keyword(s):

Brassica Rapa ◽

Addition Lines ◽

Alien Addition Lines

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Development of alien addition lines from Cucumis hystrix in Cucumis sativus: cytological and molecular marker analyses

Genome ◽

10.1139/gen-2020-0035 ◽

2020 ◽

Vol 63 (12) ◽

pp. 629-641

Author(s):

Mengxue Li ◽

Qinzheng Zhao ◽

Yuxi Liu ◽

Xiaodong Qin ◽

Wei Hu ◽

...

Keyword(s):

Cucumis Sativus ◽

Wild Species ◽

Chromosome Doubling ◽

Addition Lines ◽

Cytological Evidence ◽

Cucumis Hystrix ◽

Mother Cells ◽

Chromosome Segments ◽

Alien Addition Lines

Transferring desired genes from wild species to cultivars through alien addition lines (AALs) has been shown to be an effective method for genetic improvement. Cucumis hystrix Chakr. (HH, 2n = 24) is a wild species of Cucumis that possesses many resistant genes. A synthetic allotetraploid species, C. hytivus (HHCC, 2n = 38), was obtained from the cross between cultivated cucumber, C. sativus (CC, 2n = 14), and C. hystrix followed by chromosome doubling. Cucumis sativus – C. hystrix AALs were developed by continuous backcrossing to the cultivated cucumbers. In this study, 10 different types of AALs (CC-H01, CC-H06, CC-H08, CC-H10, CC-H12, CC-H06+H09, CC-H06+H10, CC-H06+H12, CC-H08+H10, CC-H01+H06+H10) were identified based on the analysis of fluorescence in situ hybridization (FISH) and molecular markers specific to C. hystrix chromosomes. And the behavior of the alien chromosomes in three AALs (CC-H01, CC-H06+H10, CC-H01+H06+H10) at meiosis was investigated. The results showed that alien chromosomes paired with C. sativus chromosome in few pollen mother cells (PMCs). Further, disomic alien addition lines (DAALs) carrying a pair of C. hystrix chromosome H10 were screened from the selfed progenies of CC-H10. Chromosome pairing between genomes provides cytological evidence for the possible introgression of alien chromosome segments. The development of AALs could serve as a key step for exploiting and utilizing valuable genes from C. hystrix.

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