A library of Solanum lycopersicoides introgression lines in cultivated tomato

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 685-697 ◽  
Author(s):  
Michael A Canady ◽  
Vladimir Meglic ◽  
Roger T Chetelat
Keyword(s):  
Lycopersicon Esculentum ◽  
Segregation Distortion ◽  
Germ Line ◽  
Introgression Lines ◽  
Primary Group ◽  
Solanum Lycopersicoides ◽  
Minimum Number ◽  
Map Resolution ◽  
Strong Segregation ◽  
Chromosome Segments

A set of introgression lines (ILs), containing individual chromosome segments from the wild nightshade Solanum lycopersicoides bred into the genetic background of cultivated tomato (Lycopersicon esculentum), has been developed. A primary group of 56 lines was selected for maximum representation of the S. lycopersicoides genome (~96% of the total map units), homozygosity, and a minimum number of introgressed segments per line. A secondary set of 34 lines provides increased map resolution in certain regions. Approximately 34% of the lines were sterile in the homozygous condition, but could be maintained by heterozygotes. To facilitate identification of segregating ILs, restriction fragment length polymorphism probes were converted to higher throughput cleaved amplified polymorphic sequence markers, which supplement allozyme and morphological loci. Strong segregation distortion was observed in F2 progeny of heterozygous ILs, with an excess of L. esculentum alleles in most regions. For introgressions on distal chromosome 1L, a preferential transmission of S. lycopersicoides alleles was observed in the male germ line. Homozygous ILs generally yielded less seed from self pollination than corresponding heterozygotes, indicating that sterility effects were recessive. This IL library provides a novel resource for genetic studies of traits found in S. lycopersicoides.Key words: Lycopersicon esculentum, Solanum lycopersicum, Solanum lycopersicoides, segregation distortion, alien introgression.

scholarly journals Alien introgression and morpho-agronomic characterization of diploid progenies of Solanum lycopersicoides monosomic alien addition lines (MAALs) toward pre-breeding applications in tomato (S. lycopersicum)

2021 ◽  
Author(s):  
Puneet Kaur Mangat ◽  
Junghyun Shim ◽  
Ritchel B. Gannaban ◽  
Joshua J. Singleton ◽  
Rosalyn B. Angeles-Shim
Keyword(s):  
Chromosome Complement ◽  
Introgression Lines ◽  
Addition Lines ◽  
Solanum Lycopersicoides ◽  
Drought Tolerant ◽  
Use Efficiency ◽  
Tomato Breeding ◽  
Chromosome Segments ◽  
Alien Addition Lines

Abstract Key message Alien introgressions that were captured in the genome of diploid plants segregating from progenies of monosomic alien addition lines of S. lycopersicoides confer novel phenotypes with commercial and agronomic value in tomato breeding. Abstract Solanum lycopersicoides is a wild relative of tomato with a natural adaptation to a wide array of biotic and abiotic challenges. In this study, we identified and characterized diploid plants segregating from the progenies of monosomic alien addition lines (MAALs) of S. lycopersicoides to establish their potential as donors in breeding for target trait improvement in tomato. Molecular genotyping identified 28 of 38 MAAL progenies having the complete chromosome complement of the cultivated tomato parent and limited chromosome introgressions from the wild S. lycopersicoides parent. Analysis of SSR and indel marker profiles identified 34 unique alien introgressions in the 28 MAAL-derived introgression lines (MDILs) in the genetic background of tomato. Conserved patterns of alien introgressions were detected among sibs of MDILs 2, 3, 4 and 8. Across MDILs, a degree of preferential transmission of specific chromosome segments was also observed. Morphologically, the MDILs closely resembled the cultivated tomato more than S. lycopersicoides. The appearance of novel phenotypes in the MDILs that are lacking in the cultivated parent or the source MAALs indicates the capture of novel genetic variation by the diploid introgression lines that can add commercial and agronomic value to tomato. In particular, screening of representative MDILs for drought tolerance at the vegetative stage identified MDIL 2 and MDIL 11III as drought tolerant based on visual scoring. A regulated increase in stomatal conductance of MDIL 2 under drought stress indicates better water use efficiency that allowed it to survive for 7 days under 0% moisture level.

scholarly journals A Solanum lycopersicoides reference genome facilitates biological discovery in tomato

2020 ◽  
Author(s):  
Adrian F. Powell ◽  
Lance E. Courtney ◽  
Maximilian H.-W. Schmidt ◽  
Ari Feder ◽  
Alexander Vogel ◽  
...  
Keyword(s):  
Pathogen Resistance ◽  
Wild Relatives ◽  
Introgression Lines ◽  
Tomato Genome ◽  
Nutrient Quality ◽  
Solanum Lycopersicoides ◽  
Genomic Studies ◽  
Biological Discovery ◽  
Cultivated Species ◽  
Tomato Breeding

SummaryWild relatives of tomato are a valuable source of natural variation in tomato breeding, as many can be hybridized to the cultivated species (Solanum lycopersicum). Several, including Solanum lycopersicoides, have been crossed to S. lycopersicum for the development of ordered introgression lines (ILs). Despite the utility of these wild relatives and their associated ILs, limited finished genomes have been produced to aid genetic and genomic studies. We have generated a chromosome-scale genome assembly for Solanum lycopersicoides LA2951 using PacBio sequencing, Illumina, and Hi-C. We identified 37,938 genes based on Illumina and Isoseq and compared gene function to the available cultivated tomato genome resources, in addition to mapping the boundaries of the S. lycopersicoides introgressions in a set of cv. VF36 x LA2951 introgression lines (IL). The genome sequence and IL map will support the development of S. lycopersicoides as a model for studying fruit nutrient/quality, pathogen resistance, and environmental stress tolerance traits that we have identified in the IL population and are known to exist in S. lycopersicoides.

scholarly journals A Chlorophyll Fluorescence Screening Test to Evaluate Chilling Tolerance in Tomato

HortScience ◽  
1990 ◽  
Vol 25 (3) ◽  
pp. 334-339 ◽  
Author(s):  
Mark A. Walker ◽  
Dale M. Smith ◽  
K. Peter Pauls ◽  
Bryan D. McKersie
Keyword(s):  
Chlorophyll Fluorescence ◽  
Low Temperature ◽  
Lycopersicon Esculentum ◽  
Temperature Stress ◽  
Low Temperatures ◽  
Screening Test ◽  
Full Range ◽  
Chilling Tolerance ◽  
Solanum Lycopersicoides ◽  
Commercial Cultivars

The chilling tolerance of commercial Lycopersicon esculentum cultivars (H2653, H722), Solanum lycopersicoides, an F1 hybrid of S. lycopersicoides × Sub-Arctic Maxi, and 25 BC2F2 lines of L. hirsutum × H722 (backcrossed twice to H722) was evaluated using a chlorophyll fluorescence assay. The ratio of the initial to the peak fluorescence (Fo: Fp) measured from fully expanded leaves was chosen as an indicator of plant health. Chilling induced an increase in Fo: Fp that was correlated with the sensitivity of the plant to low-temperature stress. Values of Fo: Fp remained low for cold-treated S. lycopersicoides and the F1 hybrid, which showed few symptoms of chilling-related damage, whereas the commercial cultivars, which were essentially intolerant to low temperatures, had large increases in Fo: Fp. A full range of Fo: Fp values was measured in the 25 BC2F2 lines, indicating that some chilling tolerance from the L. hirsutum parent was expressed by plants in these populations.

Isozyme analysis, chromosome pairing, and fertility of Lycopersicon esculentum × Solanum lycopersicoides diploid backcross hybrids

Genome ◽  
1989 ◽  
Vol 32 (5) ◽  
pp. 783-790 ◽  
Author(s):  
Roger T. Chetelat ◽  
Charles M. Rick ◽  
Joseph W. DeVerna
Keyword(s):  
Lycopersicon Esculentum ◽  
Chromosome Pairing ◽  
Linkage Mapping ◽  
Intergeneric Hybridization ◽  
Morphological Markers ◽  
Stainable Pollen ◽  
Mendelian Segregation ◽  
Solanum Lycopersicoides ◽  
Novel Traits ◽  
Backcross Hybrids

The wild nightshade Solanum lycopersicoides possesses potentially valuable traits for tomato (Lycopersicon esculentum) improvement. Male sterility of the intergenic F1 hybrid and its stylar rejection of tomato pollen have until recently prevented introgression. In the work herein described, ovule fertility was detected in the F1 hybrid following pollinations with S. lycopersicoides. Pollinations of the F1 with L. pennellii derived bridging hybrids resulted in a BC1 population of 65 plants. BC2 seed was obtained from most BC1 individuals. Male fertility of the BC1 plants ranged from 0 to 66% acetocarmine-stainable pollen. Of the 43 plants scored, 41 were diploids (2n = 24) and 2 were aneuploids (2n = 25 and 2n = 26). Chromosome pairing of the BC1 at diakinesis and metaphase I averaged 11.1 bivalents per pollen mother cell. BC1 and BC2 plants were scored for 19 isozyme and morphological markers covering 10 of the 12 S. lycopersicoides chromosomes. Significant deviations from Mendelian segregation were observed for seven loci, with an excess of homozygotes (+/+) in all cases. Wa, which controls the white anther trait of S. lycopersicoides, was mapped to within 15 centiMorgans (cM) of Got-4, while Mdh-4 was closely linked to Pgi-1 (<2 cM). Plants heterozygous for the S. lycopersicoides allele (+/s) at Dia-2, Skdh-1, Wa, and Aco-1 showed significantly lower pollen stainability than homozygotes (+/+). Skdh-1 and Got-3 heterozygotes had less chromosome pairing at meiosis than homozygotes. These hybrids will permit the introgression of novel traits from S. lycopersicoides into tomato.Key words: tomato, segregation, linkage mapping, quantitative trait loci, intergeneric hybridization.

scholarly journals QTL Mapping of Grain Quality Traits Using Introgression Lines Carrying Oryza rufipogon Chromosome Segments in Japonica Rice

Rice ◽  
2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Yeo-Tae Yun ◽  
Chong-Tae Chung ◽  
Young-Ju Lee ◽  
Han-Jung Na ◽  
Jae-Chul Lee ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Grain Quality ◽  
Introgression Lines ◽  
Oryza Rufipogon ◽  
Japonica Rice ◽  
Quality Traits ◽  
Chromosome Segments

scholarly journals Fertile Somatic Hybrids between F1(Lycopersicon esculentum*L. peruvianum var. humifusum) and Solanum lycopersicoides.

1997 ◽  
Vol 47 (4) ◽  
pp. 327-333
Author(s):  
Asako Matsumoto ◽  
Shigeru Imanishi ◽  
Monzur Hossain ◽  
Alice Escalante ◽  
Hiroaki Egashira
Keyword(s):  
Lycopersicon Esculentum ◽  
Somatic Hybrids ◽  
Solanum Lycopersicoides

Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers

Genome ◽  
1995 ◽  
Vol 38 (1) ◽  
pp. 166-176 ◽  
Author(s):  
G. M. Garcia ◽  
H. T. Stalker ◽  
G. Kochert
Keyword(s):  
Arachis Hypogaea ◽  
Rapd Markers ◽  
Rflp Analysis ◽  
Introgression Lines ◽  
Hybrid Population ◽  
Rflp Markers ◽  
Alien Gene Transfer ◽  
Arachis Hypogaea L ◽  
Alien Gene ◽  
Chromosome Segments

Forty-six introgression lines (F10C9) from a cross between Arachis hypogaea L. (2n = 4x = 40) and A. cardenasii Krapov. &W.C. Gregory (2n = 2x = 20) were analyzed for the introgression of A. cardenasii chromosome segments. Seventy-three RFLP probes and 70 RAPD primers, expressing from one to four A. cardenasii-specific bands, were used to evaluate the set of introgression lines. Thirty-four RFLP probes and 45 RAPD primers identified putative A. cardenasii introgressed chromosome segments in one or more lines. Introgressed segments were detected by RFLP analysis in 10 of the 11 linkage groups; the smallest introgressed fragments were detected by single RFLP markers and the largest were detected by three or four adjacent markers and represented introgressed segments of 30–40 cM. Similar results were obtained with RAPD markers, although markers detecting introgressed fragments could not be placed on the peanut linkage map. Introgression into both A. hypogaea genomes was detected and its implication in breeding for disease resistance is discussed.Key words: peanut, Arachis hypogaea, Arachis cardenasii, RFLPs, RAPDs, introgression, reciprocal recombination, translocation, alien gene transfer, wide cross.

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