Location and mapping of the powdery mildew resistance gene MlRE and detection of a resistance QTL by bulked segregant analysis (BSA) with microsatellites in wheat

2000 ◽  
Vol 100 (8) ◽  
pp. 1217-1224 ◽  
Author(s):  
N. Chantret ◽  
P. Sourdille ◽  
M. Röder ◽  
M. Tavaud ◽  
M. Bernard ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Resistance Qtl

Identification of coupling and repulsion phase RAPD markers for powdery mildew resistance gene er-1 in pea

Genome ◽  
1998 ◽  
Vol 41 (3) ◽  
pp. 440-444 ◽  
Author(s):  
K R Tiwari ◽  
G A Penner ◽  
T D Warkentin
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Rapd Markers ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Powdery Mildew Resistance Gene ◽  
Erysiphe Pisi ◽  
Mildew Resistance ◽  
Repulsion Phase

Powdery mildew is a serious disease of pea caused by the obligate parasite Erysiphe pisi Syd. Random amplified polymorphic DNA (RAPD) analysis has emerged as a cost-effective and efficient marker system. The objective of this study was to identify RAPD markers for powdery mildew resistance gene er-1. The resistant cultivar Highlight (carrying er-1) and the susceptible cultivar Radley were crossed, and F3 plants were screened with Operon (OP) and University of British Columbia (UBC) primers, using bulked segregant analysis. A total of 416 primers were screened, of which amplicons of three Operon primers, OPO-18, OPE-16, and OPL-6, were found to be linked to er-1. OPO-181200 was linked in coupling (trans to er-1) and no recombinants were found. OPE-161600 (4 ± 2 cM) and OPL-61900 (2 ± 2 cM) were linked in repulsion (cis to er-1). The fragments OPO-181200 and OPE-161600 were sequenced and specific primers designed. The specific primer pair Sc-OPO-181200 will be useful in identifying homozygous resistant individuals in F2 and subsequent segregating generations. Sc-OPE-161600 will have greatest utility in selecting heterozygous BC\dn6 nF1 individuals in backcross breeding programs.Key words: bulked segregant analysis,Erysiphe pisi, pea, RAPD.

Molecular Mapping of Powdery Mildew Resistance Gene MlWE18 in Wheat Originated from Wild Emmer (Triticum turgidum var. dicoccoides)

2009 ◽  
Vol 35 (10) ◽  
pp. 1791-1797 ◽  
Author(s):  
Jun HAN ◽  
Lian-Song ZHANG ◽  
Gen-Qiao LI ◽  
Hong-Tao ZHANG ◽  
Chao-Jie XIE ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Molecular Mapping ◽  
Triticum Turgidum ◽  
Wild Emmer ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Identification of Powdery Mildew Resistance Gene in Pea Line X9002

2015 ◽  
Vol 41 (4) ◽  
pp. 515 ◽  
Author(s):  
Zhong-Yi WANG ◽  
Hai-Ning FU ◽  
Su-Li SUN ◽  
Can-Xin DUAN ◽  
Xiao-Fei WU ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance
Sign in / Sign up

Export Citation Format

Share Document