Background:
Multi-vitamin formulations are indicated for the elderly, adults, children’s and infants; to prevent or restore vitamin deficiencies.
Objective:
To provide a validated high-throughput method for simultaneous determination of vitamins in multi-vitamin formulations.
Methods:
A high-throughput UPLC-ESI-MS method was developed and validated for simultaneous determination of vitamins B1, B2, B3, B5, B6, B7 and B9. Analytes were eluted on an Acquity UPLC®BEH C18 1.7 μm, 2.1 x 50 mm column at 40 ± 5°C. Mobile phase containing acetonitrile (0.1% formic acid) and water (0.1% formic acid) in 30:70% ratio was pumped at 300 μL/min, under isocratic control. Protonated ions of vitamin B1, B2, B3, B5, B6, B7 and B9 were monitored in single ion recording mode, using electrospray ionization probe in positive mode.
Results:
The m/z ratio of positive ions of vitamin B1, B2, B3, B5, B6, B7 and B9 were 265.1, 377.2, 122.95, 220, 169.98, 244.9, and 442.1; respectively. The calibration curve of different linearity range (ng/ml) was prepared for each vitamin. Linearity range for vitamin B1, B2, B3, B5, B6, B7 and B9 were 60-1000, 25-1000, 75-5000, 30-1000 and 25-1000 ng/mL; respectively. Coefficient of variation for intra-day and inter-day precision for vitamin B1, B2, B3, B5, B6, B7 and B9; at middle and higher limit of quantitation were less than 15%.
Conclusion:
The method was successfully developed and validated, and three different brands of multi-vitamin tablets were assayed for water soluble vitamins.
Simultaneous Determination of Sulphur metabolites in Arabidopsis thaliana via LC-ESI-MS/MS and 34S-metabolic Labelling