High-throughput, Determination of Vitamins (B1, B2, B3, B5, B6, B7 and B9) Using UPLC-ESI-MS.

Current Chromatography ◽

10.2174/2213240608666210203112120 ◽

2021 ◽

Vol 08 ◽

Author(s):

Abdullah S. Al-Dosseri ◽

Mohd Aftab Alam ◽

Fahad I. Al-Jenoobi

Keyword(s):

Formic Acid ◽

Simultaneous Determination ◽

High Throughput ◽

Vitamin B1 ◽

Water Soluble ◽

Vitamin Deficiencies ◽

Esi Ms ◽

Linearity Range ◽

Limit Of Quantitation

Background: Multi-vitamin formulations are indicated for the elderly, adults, children’s and infants; to prevent or restore vitamin deficiencies. Objective: To provide a validated high-throughput method for simultaneous determination of vitamins in multi-vitamin formulations. Methods: A high-throughput UPLC-ESI-MS method was developed and validated for simultaneous determination of vitamins B1, B2, B3, B5, B6, B7 and B9. Analytes were eluted on an Acquity UPLC®BEH C18 1.7 μm, 2.1 x 50 mm column at 40 ± 5°C. Mobile phase containing acetonitrile (0.1% formic acid) and water (0.1% formic acid) in 30:70% ratio was pumped at 300 μL/min, under isocratic control. Protonated ions of vitamin B1, B2, B3, B5, B6, B7 and B9 were monitored in single ion recording mode, using electrospray ionization probe in positive mode. Results: The m/z ratio of positive ions of vitamin B1, B2, B3, B5, B6, B7 and B9 were 265.1, 377.2, 122.95, 220, 169.98, 244.9, and 442.1; respectively. The calibration curve of different linearity range (ng/ml) was prepared for each vitamin. Linearity range for vitamin B1, B2, B3, B5, B6, B7 and B9 were 60-1000, 25-1000, 75-5000, 30-1000 and 25-1000 ng/mL; respectively. Coefficient of variation for intra-day and inter-day precision for vitamin B1, B2, B3, B5, B6, B7 and B9; at middle and higher limit of quantitation were less than 15%. Conclusion: The method was successfully developed and validated, and three different brands of multi-vitamin tablets were assayed for water soluble vitamins.

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Simultaneous Determination of Four Herbicide and Pesticide Residues in Chinese Green Tea Using QuEChERS Purification Procedure and UPLC-MS/MS Analysis

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.634-638.1586 ◽

2013 ◽

Vol 634-638 ◽

pp. 1586-1590

Author(s):

Su Fang Wang ◽

Shou Jie Zhang ◽

Chun Hong Dong ◽

Guo Qing Wang ◽

Jun Feng Guo ◽

...

Keyword(s):

Formic Acid ◽

Simultaneous Determination ◽

Green Tea ◽

Limit Of Detection ◽

Multiple Reaction Monitoring ◽

Graphitized Carbon Black ◽

Ms Analysis ◽

Relative Standard ◽

Limit Of Quantitation

A method for simultaneous determination of residuals of four herbicides and pesticides, simazine, carboxin, diflubenzuron and rotenone, in Chinese green tea was developed. In the proposed method, the tea powder was placed in a centrifuge tube with a plug, extracted in saturated aqueous sodium chloride solution and acetonitrile, agitated using vortex oscillator, and then centrifuged 5 min at 4000 rpm. The supernatant solution was purified by primary secondary amine (PSA) sorbent, C18 power, and graphitized carbon black powder, respectively. Then the purified extracts were dissolved with acetonitrile:0.1% formic acid aqueous solution (40:60, V/V) and agitated, filtered using a syringe with 0.22 μm nylon filter prior to UPLC-MS/MS analysis. The UPLC analysis was performed on an ACQUITY UPLC® HSS T3 column (2.1 mm×100 mm, 1.8 µm), using acetonitrile-0.1% formic acid as mobile phase with the flow rate as 0.3 mL•min-1. Injection volume was 10 µL. Positive ionization mode was applied, and the ions were monitored in the multiple reaction monitoring (MRM) mode with curtain gas 0.069 MPa, collision gas 0.052 MPa, ESI ion spray voltage 5000 V, temperature 550 °C, nebulizer gas 0.24 MPa, and turbo gas 0.28 MPa. The limit of detection (LOD) and limit of quantitation (LOQ) of the proposed method are 1 μg•kg-1and 5 μg•kg-1, respectively. The average recoveries of the four pesticides at 10, 20, and 50 µg•kg-1spiking levels range from 77.4% to 95.3%. TheSupersSuperscript textcript textrelative standard deviation (RSD) (n=6) range form 11.83% to 4.52%.

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Simultaneous Determination of Water-Soluble Vitamins (Vitamin B1, B2, B3, B6and C) in Dietary Supplements by High-Performance Liquid Chromatography

The Korean Journal of Food And Nutrition ◽

10.9799/ksfan.2011.24.3.414 ◽

2011 ◽

Vol 24 (3) ◽

pp. 414-421 ◽

Cited By ~ 1

Author(s):

Hee-Jae Suh ◽

So-Hee Kim

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Dietary Supplements ◽

Simultaneous Determination ◽

High Performance ◽

Vitamin B1 ◽

Water Soluble

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Simultaneous determination of dl-lactic acid and dl-3-hydroxybutyric acid enantiomers in saliva of diabetes mellitus patients by high-throughput LC–ESI-MS/MS

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-012-6320-0 ◽

2012 ◽

Vol 404 (6-7) ◽

pp. 1925-1934 ◽

Cited By ~ 41

Author(s):

Haruhito Tsutsui ◽

Toshiki Mochizuki ◽

Toshio Maeda ◽

Ichiro Noge ◽

Yutaka Kitagawa ◽

...

Keyword(s):

Diabetes Mellitus ◽

Lactic Acid ◽

Simultaneous Determination ◽

High Throughput ◽

Hydroxybutyric Acid ◽

Esi Ms

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Determination of Glutamate and GABA Released by Mouse Embryonic Stem Cells Using HILIC-ESI-MS/MS

The Natural Products Journal ◽

10.2174/2210315509666190211123132 ◽

2020 ◽

Vol 10 (2) ◽

pp. 122-129

Author(s):

Haoyu Lv ◽

Yabin Tang ◽

Fan Sun ◽

Shimin An ◽

Xinjie Yang ◽

...

Keyword(s):

Stem Cells ◽

Embryonic Stem Cells ◽

Embryonic Stem ◽

Mouse Embryonic Stem Cells ◽

Reversed Phase ◽

Water Soluble ◽

Substitution Matrix ◽

Neural Cells ◽

Esi Ms

Background:In recent years, more and more researches have shown that neurotransmitters can also be synthesized and released by peripheral non-neural cells. However, specificity and high sensitivity detection means were required for confirming ESCs autocrine glutamate and γ - aminobutyric acid (GABA). Glutamate and GABA are water-soluble and polar compounds which cannot be retained on a reversed phase C18 column, and their contents are often at a trace level. On the other hand, the biological matrix such as cell culture fluid contains a large number of amino acids, vitamins, carbohydrates, inorganic ions and other substances. Therefore, the main problem is the selection of the chromatographic column to avoid matrix interference.Objective:To establish a rapid and reliable method for the simultaneous determination of glutamate and GABA released from embryonic stem cells based on analytical chemistry.Methods:Glutamate and GABA released from mouse embryonic stem cells were determined on the basis of hydrophilic interaction chromatography coupled with electrospray ionization tandem Mass Spectrometry (HILIC- ESI- MS/MS), using isotope internal standards and substitution matrix method.Results:Undifferentiated embryonic stem cells autocrine glutamate and GABA and will reach releasing- reuptacking dynamic equilibriums at different time points. In contrast, neither glutamate nor GABA releasing could be detected from the MEFs, indicating the specificity release of the mESCs in the applied analytic method.Conclusion:A novel, simple, sensitive, selective and quantitative method was developed for determination of the glutamate and GABA from mouse embryonic stem cells.

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Reverse phase-liquid chromatography assisted protocol for simultaneous determination of lamivudine and tenofovir disoproxil fumarate in combined medication used to control HIV infection: an investigative approach

Future Journal of Pharmaceutical Sciences ◽

10.1186/s43094-021-00233-3 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Vaibhav S. Adhao ◽

Suraj R. Chaudhari ◽

Jaya P. Ambhore ◽

Sunil Sangolkar ◽

Raju R. Thenge ◽

...

Keyword(s):

Liquid Chromatography ◽

Hiv Infection ◽

Simultaneous Determination ◽

Tenofovir Disoproxil Fumarate ◽

Solvent System ◽

Array Detector ◽

Technical Requirements ◽

Relative Standard ◽

Limit Of Quantitation

Abstract Background Human immunodeficiency virus (HIV) causes severe life-threatening condition, i.e., AIDS. HIV destabilises an individual’s ability to prevent infection. Therefore, the combine medication lamivudine (LVD) and tenofovir disoproxil fumarate (TDF) are prescribed to suppress the amount of HIV infection in individual’s body; thus, the individual’s immune system could function properly. Consequently, the objective of present research work was to investigate robust and sensitive liquid chromatography avenue for simultaneous determination of lamivudine and tenofovir disoproxil fumarate in pure material and combined dosage form. Results The reversed-phase chromatographic separation has been performed through Hypersil BDS C18 column using solvent system composed of 10 mM potassium dihydrogen phosphate (pH 4.0): acetonitrile (60:40% v/v). The determination was executed at 30 oC at 1 mL/min rate for flow of solvent system through column. The eluents of column were monitored at 265 nm using Photodiode Array detector has revealed admirable retention times, i.e., 4.67 and 8.78 min for both drugs, respectively. The calibration curve demonstrated excellent linearity in the range of 10–50 μg/mL for lamivudine and tenofovir disoproxil fumarate with better determination coefficients was more than (r2 0.999). Conclusion The estimable method was effectively validated with respect to accuracy, precision, sensitive (limit of detection and limit of quantitation), robustness, ruggedness, and for selectivity and specificity. The value less than 2 for percentage relative standard deviation for accuracy, precision, robustness, and ruggedness satisfying the acceptance criteria as per procedure of International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use.

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