Variations of l- and d-amino acid levels in the brain of wild-type and mutant mice lacking d-amino acid oxidase activity

Over the years, accumulating evidence has indicated that D-serine represents the main endogenous ligand of NMDA (N-methyl-D-aspartate) receptors. In the brain, the concentration of D-serine stored in cells is defined by the activity of two enzymes: serine racemase (responsible for both the synthesis and degradation) and D-amino acid oxidase (which catalyses D-serine degradation). The present review is focused on human D-amino acid oxidase, discussing the mechanisms involved in modulating enzyme activity and stability, with the aim to substantiate the pivotal role of D-amino acid oxidase in brain D-serine metabolism.

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Determination of Free -Proline and -Leucine in the Brains of Mutant Mice Lacking -Amino Acid Oxidase Activity

Analytical Biochemistry ◽

10.1006/abio.2001.5382 ◽

2001 ◽

Vol 298 (2) ◽

pp. 253-258 ◽

Cited By ~ 63

Author(s):

Kenji Hamase ◽

Tomomi Inoue ◽

Akiko Morikawa ◽

Ryuichi Konno ◽

Kiyoshi Zaitsu

Keyword(s):

Amino Acid ◽

Oxidase Activity ◽

Mutant Mice ◽

Acid Oxidase ◽

Free Proline ◽

Amino Acid Oxidase

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Excessive urinary excretion of methionine in mutant mice lacking d-amino-acid oxidase activity

Metabolism ◽

10.1016/0026-0495(88)90190-4 ◽

1988 ◽

Vol 37 (12) ◽

pp. 1139-1142 ◽

Cited By ~ 16

Author(s):

Ryuichi Konno ◽

Kazumasa Isobe ◽

Akira Niwa ◽

Yosihiro Yasumura

Keyword(s):

Amino Acid ◽

Urinary Excretion ◽

Oxidase Activity ◽

Mutant Mice ◽

Acid Oxidase ◽

Amino Acid Oxidase

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MOUSE MUTANT DEFICIENT IN d-AMINO ACID OXIDASE ACTIVITY

Genetics ◽

10.1093/genetics/103.2.277 ◽

1983 ◽

Vol 103 (2) ◽

pp. 277-285 ◽

Cited By ~ 1

Author(s):

Ryuichi Konno ◽

Yosihiro Yasumura

Keyword(s):

Enzyme Activity ◽

Amino Acid ◽

Oxidase Activity ◽

Null Allele ◽

Gene Dosage ◽

Autosomal Gene ◽

Acid Oxidase ◽

Wild Type ◽

Amino Acid Oxidase ◽

Gene Dosage Effect

ABSTRACT d-Amino acid oxidase activity in the kidney homogenates of mice of seven strains was measured to search for a mutant for this enzyme. There was a consistent sex difference in the enzyme activity in these strains: male mice showed higher levels of the enzyme activity than females. In contrast to other strains, some mice of the ddY strain did not possess enzyme activity. This trait was inheritable, and a mouse stock without enzyme activity (DAO-) was established. The allele (Dao-1c) carried by the DAO- mice was recessive and behaved as a single autosomal gene in inheritance. Heterozygous mice for this gene (Dao-1 +/Dao-1c) showed nearly half the enzyme activity of the wild-type homozygotes (Dao-1 +/Dao-1 +), suggesting that Dao-1c is a null allele and that there is a gene dosage effect on the enzyme activity.

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