scholarly journals Comparison of methods for quantitative biomolecular interaction analysis

2021 ◽  
Author(s):  
Monika Conrad ◽  
Peter Fechner ◽  
Günther Proll ◽  
Günter Gauglitz
Keyword(s):  
Interaction Analysis ◽  
Simulated Data ◽  
Evaluation Procedure ◽  
Biomolecular Interaction ◽  
Experimental Conditions ◽  
Biomolecular Interaction Analysis ◽  
Advantages And Disadvantages ◽  
First Order Kinetics ◽  
Kinetic Rate Constants ◽  
The Individual

AbstractIn order to perform good kinetic experiments, not only the experimental conditions have to be optimized, but the evaluation procedure as well. The focus of this work is the in-depth comparison of different approaches and algorithms to determine kinetic rate constants for biomolecular interaction analysis (BIA). The different algorithms are applied not only to flawless simulated data, but also to real-world measurements. We compare five mathematical approaches for the evaluation of binding curves following pseudo-first-order kinetics with different noise levels. In addition, reflectometric interference spectroscopy (RIfS) measurements of two antibodies are evaluated to determine their binding kinetics. The advantages and disadvantages of the individual approach will be investigated and discussed in detail. In summary, we will raise awareness on how to evaluate and judge results from BIA by using different approaches rather than having to rely on “black box” closed (commercial) software packages.

scholarly journals Polypyrrole–Peptide Microarray for Biomolecular Interaction Analysis by SPR Imaging

2009 ◽  
pp. 317-328 ◽  
Author(s):  
Marie-Bernadette Villiers ◽  
Sandra Cortès ◽  
Carine Brakha ◽  
Patrice Marche ◽  
André Roget ◽  
...  
Keyword(s):  
Interaction Analysis ◽  
Biomolecular Interaction ◽  
Peptide Microarray ◽  
Biomolecular Interaction Analysis ◽  
Spr Imaging

scholarly journals Thermal Performance Evaluation of Common Exterior Residential Wall Types in Egypt

Buildings ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 95
Author(s):  
Khaled Tarabieh ◽  
Ahmed Aboulmagd
Keyword(s):  
Thermal Performance ◽  
Energy Efficient ◽  
State Of The Art ◽  
Experimental Testing ◽  
Simulated Data ◽  
Experimental Tests ◽  
Evaluation Procedure ◽  
Materials Used ◽  
The Individual ◽  
Composite Walls

The demand for energy-efficient housing is on the rise in Egypt. The information about the individual materials used in the construction of typical residential wall assemblies are known in the literature. However, data from lab tests to validate the performance for whole composite walls are limited. Three typical wall types were constructed and tested utilizing a standard experimental evaluation procedure based on thermal convection loads. A research framework combining the validated thermal performance data from the experimental test and the simulated data was developed. The experimental tests were performed utilizing a state-of-the-art guarded hotbox apparatus and following the guidelines of the ASTM C1363-11 standard. The solar radiation load was taken into account in the calculations according to the standard, and the error estimation and uncertainty analysis for the experimental tests are reported. The results of the experimental testing are described and a recommendation of the best wall type is noted. The output of this research will help to initiate a material database of the thermal performance of typical residential wall types used in Egypt that have been validated in the lab. This will be useful for the building industry as a whole to understand the performance of the materials in composite assemblies and their impact on energy efficiency.

Biomolecular Interaction Analysis

Methods ◽  
1994 ◽  
Vol 6 (2) ◽  
pp. 95-98 ◽  
Author(s):  
Magnus Malmqvist ◽  
Russ Granzow
Keyword(s):  
Interaction Analysis ◽  
Biomolecular Interaction ◽  
Biomolecular Interaction Analysis
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