Advantages And Disadvantages
Recently Published Documents
AbstractThe clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (CRISPR/Cas9) gene editing technology, as a revolutionary breakthrough in genetic engineering, offers a promising platform to improve the treatment of various genetic and infectious diseases because of its simple design and powerful ability to edit different loci simultaneously. However, failure to conduct precise gene editing in specific tissues or cells within a certain time may result in undesirable consequences, such as serious off-target effects, representing a critical challenge for the clinical translation of the technology. Recently, some emerging strategies using genetic regulation, chemical and physical strategies to regulate the activity of CRISPR/Cas9 have shown promising results in the improvement of spatiotemporal controllability. Herein, in this review, we first summarize the latest progress of these advanced strategies involving cell-specific promoters, small-molecule activation and inhibition, bioresponsive delivery carriers, and optical/thermal/ultrasonic/magnetic activation. Next, we highlight the advantages and disadvantages of various strategies and discuss their obstacles and limitations in clinical translation. Finally, we propose viewpoints on directions that can be explored to further improve the spatiotemporal operability of CRISPR/Cas9.
Analisis Pengaruh Model Pembelajaran Contextual Teaching and Learning Untuk Meningkatkan Hasil Belajar Siswa
This study aims to: to determine the meaning of the CTL approach, the principles of the CTL approach, the characteristics of the CTL approach, the components in the CTL, the objectives of the CTL approach, how the steps of the CTL approach, as well as the advantages and disadvantages of the CTL approach, and the effect of the Learning Model. CTL to Improve Student Learning Outcomes. The method used in this research is literacy (literature study) by reviewing several sources related to CTL (Contextual Teaching and Learning). The result of this study is that there is an increase in student learning outcomes using the CTL learning model
Abstract Based on the Hirota's bilinear method, a more classic limit technique is perfected to obtain second-order smooth positons. Immediately afterwards, we propose an extremely ingenious limit approach in which higher-order smooth positons and breather positons can be quickly derived from N-soliton solution. Under this ingenious technique, the smooth positons and breather positons of the modified Korteweg-de Vries system are quickly and easily derived. Compared with the generalized Darboux transformation, the approach mentioned in this paper has the following advantages and disadvantages: the advantage is that it is simple and fast; the disadvantage is that this method cannot get a concise general mathematical expression of nth-order smooth positons.
The article considers the scientific and practical aspects of customs representative institution activity in Russia in the conditions of intense and challenging foreign economic activity (FEA) of enterprises. On the basis of customs process specifics identified in Russia and comprehending approaches to the development of the customs representative institution, a role of this institution’s subjects is determined, and the ambiguity of attitude towards them from the customs authorities is represented. The complication of FEA was noted as the main factor in increasing demand for the customs representatives’ services. The accelerated digitalization of the customs process that is carried out by Russian Federal Customs Service was noticed as a factor potentially limiting their activities. There has been presented the dynamics of customs representatives in Russia, the substantive coverage is studied, and the most requested customs brokerage services are listed. Moreover, the advantages and disadvantages of engaging customs representatives by FEA participants for customs clearance are also shown in this article. Among the merits of engaging customs representatives for customs clearance are particularly highlighted competence, flexibility in working methods, and responsibility of customs representatives with the variability of legislation and technique of working. Shortcomings are the risk of malpractice in providing services and the involvement of the declarant in illegal schemes in the customs clearance process. There has been given the analysis of the current practice, considered the issues of the actual and relevant challenges in the development of customs representative institution. Special attention focuses on the influence of legislative procedures, which regulate the activity of customs representatives and affairs related to digital problems of the customs process, the internal problems connected with the work of customs representatives, such as improvement of the organizational structure and competence of the staff. There have been provided general recommendations for improving the customs intermediaries’ efficiency by revising and adjusting the principles of their interaction with customs authorities, as well as by optimizing their internal organizational structure through a competent division of responsibilities between employees.
The purpose of the article is to analyze the main results of the works that are used in the calculations of elevators with moderate speed modes, to clarify the suitability of their individual positions for developing the parameters of centrifugal unloading of high-speed elevators. Works devoted to the study of the operation of high-speed elevators, the results of which have not received a decent interpretation and development, are of considerable interest. As the efficiency of high-speed elevators is determined by the quality of centrifugal unloading and by the operation of the belt-drum mechanism without slipping, there is a need to analyze the work aimed at solving this problem. The paper presents known solutions for determining the parameters of centrifugal unloading, which are based on various hypotheses of the movement of material particles inside the bucket. The physical and mechanical phenomena that affect the movement of material particles in the elevator bucket are studied. The advantages and disadvantages of each hypothesis are revealed. The theoretical study of the process of centrifugal unloading is complicated by the fact that during the movement and exit of the material from the bucket, there is an unstable movement of the bulk material under the influence of a changing system of forces: the forces of attraction, centrifugal and coriolis forces, and the friction force. Meanwhile, even the simplest cases of material motion under a gravitational or mixed discharge regime are difficult to analyze theoretically. In this regard, the dependencies and methods of constructing the trajectories of the material movement are established, as well as the relevance of using a particular equation.
Modern Displacement Measuring Systems Used in Geotechnical Laboratories: Advantages and Disadvantages
The paper presents the contemporary displacement measurement systems used in geotechnical laboratories during the determination of soil precise mechanical parameters, e.g., the shear modules G: initial and in the range of small and very small strains. In the laboratory, researchers use standard sensors for measuring deformation, pressure, and force as well as modern measuring systems such as linear variable differential transformers (LVDT), proximity transducers (PT), magnetic encoder sensors with fiber Bragg grating (FBG), or methods based on laser or X-ray measurement. None of the measurements are universal and their use depends on the type of soil (cohesive, non-cohesive), its condition (loose or dense, stiff or very soft), and its characteristic properties (e.g., organic soil, swelling soil). This study points out the interesting equipment solutions and presents the guidelines for selecting appropriate methods of deformation measurement.
This research aims to give a clear image of coursebook evaluation in the English department of Komar University of Science and Technology (KUST) in Iraqi Kurdistan. The focus of this paper is to show the advantages and disadvantages of six coursebooks that are used in the English department of KUST. For this purpose, the researchers conducted interviews with the instructors and a survey with the students of these courses. These interviews and the survey show the good sides and the shortcomings of the studied coursebooks by asking different types of questions related to the contents of the books. The research took place in Fall-Winter 2019. The study results show that two-thirds of the teachers were satisfied with the coursebooks chosen to teach in the department of English to a large degree, and most of the students were satisfied with their coursebooks.
The main aim of this article is to study the effectiveness of using Zoom technology to prepare useful lessons to improve the success of students studying foundation of foreign languages by distance mode at a university. We would like to share our analyze the experience of using the Zoom virtual platform in the process of organizing distance learning from the point of view of the students. The article presents the results of students’ surveys that illustrate the perception of students in terms of the usefulness and ease of use of the utilized software. The article pays particular attention to the estimation of the main characteristics and didactic capabilities of the Zoom virtual platform for organizing distance learning foreign language classes at a university. The article draws conclusions on the advantages and disadvantages of the software, describes the stages of work and offers methodological recommendations as well as lists the main difficulties that teachers and students at Tashkent State University of Economics have encountered in the course of language education.
The article provides an overview and analysis of the cement industry in Uzbekistan. The authors analyzed the volume of construction work, the volume of production and sales of cement products on the exchange, as well as the target parameters of the production of cement products in 2019–2025. Particular attention is paid to the technological process of cement production, in particular dry and wet methods. The advantages and disadvantages of each production technology are presented in detail. The authors reviewed the existing and projected cement manufacturing enterprises in Uzbekistan.
The article discusses the choice of different surgical procedures in patients with locally advanced vulvar cancer, depending on the volume of resected tissues and the possibility of reconstructive plastic surgery. The advantages and disadvantages of various plastic reconstructive techniques are provided. The issue of wound closing after the vulvectomy and the plastic techniques has always been controversial and led to the sophistication of the reconstructive component, as well as to the limitations or refusal of this surgical procedure use.