Characteristics of Single, Large-Conductance Calcium-Dependent Potassium Channels (BK Ca ) from Smooth Muscle Cells Isolated from the Rabbit Mesenteric Artery

1998 ◽  
Vol 164 (2) ◽  
pp. 125-138 ◽  
Author(s):  
D.K. Mistry ◽  
C.J. Garland
Keyword(s):  
Smooth Muscle ◽  
Potassium Channels ◽  
Smooth Muscle Cells ◽  
Mesenteric Artery ◽  
Muscle Cells ◽  
Calcium Dependent ◽  
Rabbit Mesenteric Artery

Pharmacology of ATP-sensitive K+ currents in smooth muscle cells from rabbit mesenteric artery

1995 ◽  
Vol 269 (5) ◽  
pp. C1112-C1118 ◽  
Author(s):  
J. M. Quayle ◽  
A. D. Bonev ◽  
J. E. Brayden ◽  
M. T. Nelson
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mesenteric Artery ◽  
Katp Channels ◽  
Muscle Cells ◽  
K Channel ◽  
Patch Clamp Technique ◽  
Functional Studies ◽  
Rabbit Mesenteric Artery ◽  
K Currents

The inference that ATP-sensitive K+ (KATP) channels are involved in arterial responses to the synthetic K+ channel openers, hypoxia, adenosine, and calcitonin gene-related peptide, has relied on the sensitivity of these responses to the sulfonylureas glibenclamide and tolbutamide and to tetraethylammonium (TEA+). The inhibition of KATP currents by glibenclamide, tolbutamide, and TEA+ was investigated in single smooth muscle cells from rabbit mesenteric artery by use of the whole cell patch-clamp technique. The synthetic K+ channel openers pinacidil (half-activation 0.6 microM), cromakalim (half-activation 1.9 microM), and diazoxide (half-activation 37.1 microM) activated K(+)-selective currents that were blocked by glibenclamide. Elevation of pipette (intracellular) ATP concentration decreased K+ currents induced by pinacidil. Half-inhibition of KATP currents by glibenclamide and tolbutamide occurred at 101 nM and 351 microM, respectively. KATP currents were also inhibited by external TEA+, with half-inhibition at 6.2 mM. The results indicate that glibenclamide is an effective inhibitor of KATP channels in arterial smooth muscle and that tolbutamide and TEA+ are much less effective. Furthermore, these results support numerous functional studies that have demonstrated that the vasorelaxations to K+ channel openers are inhibited by < 10 microM glibenclamide but not by < 1 mM TEA+.

scholarly journals Actions of FRC-8653 on Smooth Muscle Cells of the Rabbit Mesenteric Artery

1991 ◽  
Vol 57 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Mikio Nakashima ◽  
Makoto Kou ◽  
Hikaru Hashitani ◽  
Guifa Chen ◽  
Hideharu Ono ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mesenteric Artery ◽  
Muscle Cells ◽  
Rabbit Mesenteric Artery

scholarly journals Effects of nifedipine on smooth muscle cells of the rabbit mesenteric artery.

1983 ◽  
Vol 33 ◽  
pp. 198
Author(s):  
Yuichi Kanmura ◽  
Takeo Itoh ◽  
Ilikaru Suzuki ◽  
Yushi Ito ◽  
Hirosi Kuriyama
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Mesenteric Artery ◽  
Muscle Cells ◽  
Rabbit Mesenteric Artery
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