Construction of quintuple protease gene disruptant for heterologous protein production in Aspergillus oryzae

2009 ◽  
Vol 82 (4) ◽  
pp. 691-701 ◽  
Author(s):  
Jaewoo Yoon ◽  
Shinya Kimura ◽  
Jun-ichi Maruyama ◽  
Katsuhiko Kitamoto
Keyword(s):  
Aspergillus Oryzae ◽  
Protein Production ◽  
Heterologous Protein ◽  
Heterologous Protein Production ◽  
Protease Gene

scholarly journals Enhanced Production and Secretion of Heterologous Proteins by the Filamentous Fungus Aspergillus oryzae via Disruption of Vacuolar Protein Sorting Receptor Gene Aovps10

2010 ◽  
Vol 76 (17) ◽  
pp. 5718-5727 ◽  
Author(s):  
Jaewoo Yoon ◽  
Tuerxun Aishan ◽  
Jun-ichi Maruyama ◽  
Katsuhiko Kitamoto
Keyword(s):  
Aspergillus Oryzae ◽  
Filamentous Fungus ◽  
Protein Production ◽  
Heterologous Protein ◽  
Protein Sorting ◽  
Heterologous Protein Production ◽  
Heterologous Proteins ◽  
Extracellular Production ◽  
Sorting Receptor ◽  
Vacuolar Protein

ABSTRACT Filamentous fungi have received attention as hosts for heterologous protein production because of their high secretion capability and eukaryotic posttranslational modifications. However, despite these positive attributes, a bottleneck in posttranscriptional processing limits protein yields. The vacuolar protein sorting gene VPS10 encodes a sorting receptor for the recognition and delivery of several yeast vacuolar proteins. Although it can also target recombinant and aberrant proteins for vacuolar degradation, there is limited knowledge of the effect of its disruption on heterologous protein production. In this study, cDNA encoding AoVps10 from the filamentous fungus Aspergillus oryzae was cloned and sequenced. Microscopic observation of the transformant expressing AoVps10 fused with enhanced green fluorescent protein showed that the fusion protein localized at the Golgi and prevacuolar compartments. Moreover, disruption of the Aovps10 gene resulted in missorting and secretion of vacuolar carboxypeptidase AoCpyA into the medium, indicating that AoVps10 is required for sorting of vacuolar proteins to vacuoles. To investigate the extracellular production levels of heterologous proteins, ΔAovps10 mutants expressing either bovine chymosin (CHY) or human lysozyme (HLY) were constructed. Interestingly, the ΔAovps10 mutation increased the maximum extracellular production levels of CHY and HLY by 3- and 2.2-fold, respectively. Western blot analysis of extracellular heterologous proteins also demonstrated an improvement in productivity. These results suggest that AoVps10 plays a role in the regulation of heterologous protein secretion in A. oryzae and may be involved in the vacuolar protein degradation through the Golgi apparatus.

Isolation of Aspergillus oryzae mutants for heterologous protein production from a double proteinase gene disruptant

2009 ◽  
Vol 82 (6) ◽  
pp. 1105-1114 ◽  
Author(s):  
Takashi Nemoto ◽  
Taisuke Watanabe ◽  
Yutaka Mizogami ◽  
Jun-ichi Maruyama ◽  
Katsuhiko Kitamoto
Keyword(s):  
Aspergillus Oryzae ◽  
Protein Production ◽  
Heterologous Protein ◽  
Heterologous Protein Production

Using DNA-Tagged Mutagenesis to Improve Heterologous Protein Production in Aspergillus oryzae

2000 ◽  
Vol 29 (1) ◽  
pp. 28-37 ◽  
Author(s):  
Debbie S Yaver ◽  
Michael Lamsa ◽  
Rebecca Munds ◽  
Stephen H Brown ◽  
Suzie Otani ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Protein Production ◽  
Heterologous Protein ◽  
Heterologous Protein Production

New Promoters to Improve Heterologous Protein Production in Aspergillus vadensis

2014 ◽  
Vol 3 (3) ◽  
pp. 244-251 ◽  
Author(s):  
Helena Culleton ◽  
Ourdia Bouzid ◽  
Vincent McKie ◽  
Ronald Vries
Keyword(s):  
Protein Production ◽  
Heterologous Protein ◽  
Heterologous Protein Production

Absence of See1p, a widely conservedSaccharomyces cerevisiaeprotein, confers both deficient heterologous protein production and endocytosis

Yeast ◽  
2008 ◽  
Vol 25 (12) ◽  
pp. 871-877 ◽  
Author(s):  
Cristina Martín-Granados ◽  
Sean-Patrick Riechers ◽  
Ulf Stahl ◽  
Christine Lang
Keyword(s):  
Protein Production ◽  
Heterologous Protein ◽  
Heterologous Protein Production

scholarly journals State and specific growth estimation in heterologous protein production by Pichia pastoris

AIChE Journal ◽  
2011 ◽  
Vol 58 (10) ◽  
pp. 2966-2979 ◽  
Author(s):  
José M. Barrigón ◽  
Ramon Ramon ◽  
Isabel Rocha ◽  
Francisco Valero ◽  
Eugénio C. Ferreira ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Protein Production ◽  
Heterologous Protein ◽  
Specific Growth ◽  
Heterologous Protein Production ◽  
Growth Estimation
Sign in / Sign up

Export Citation Format

Share Document