scholarly journals Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis

2011 ◽  
Vol 91 (2) ◽  
pp. 341-351 ◽  
Author(s):  
Baldo F. Cordero ◽  
Inmaculada Couso ◽  
Rosa León ◽  
Herminia Rodríguez ◽  
M. Ángeles Vargas
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Phytoene Synthase ◽  
Nuclear Transformation ◽  
Chlorella Zofingiensis ◽  
Phytoene Synthase Gene

The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation

1994 ◽  
Vol 14 (6) ◽  
pp. 4011-4019
Author(s):  
J A Nelson ◽  
P B Savereide ◽  
P A Lefebvre
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Selectable Marker ◽  
Marker Gene ◽  
Small Subunit ◽  
Direct Selection ◽  
Nuclear Transformation ◽  
Ribulose Bisphosphate Carboxylase ◽  
Bisphosphate Carboxylase ◽  
Dominant Selectable Marker ◽  
Cry1 Gene

We have cloned and sequenced the CRY1 gene, encoding ribosomal protein S14 in Chlamydomonas reinhardtii, and found that it is highly similar to S14/rp59 proteins from other organisms, including mammals, Drosophila melanogaster, and Saccharomyces cerevisiae. We isolated a mutant strain resistant to the eukaryotic translational inhibitors cryptopleurine and emetine in which the resistance was due to a missense mutation (CRY1-1) in the CRY1 gene; resistance was dominant in heterozygous stable diploids. Cotransformation experiments using the CRY1-1 gene and the gene for nitrate reductase (NIT1) produced a low level of resistance to cryptopleurine and emetine. Resistance levels were increased when the CRY1-1 gene was placed under the control of a constitutive promoter from the ribulose bisphosphate carboxylase/oxygenase small subunit 2 (RBCS2) gene. We also found that the 5' untranslated region of the CRY1 gene was required for expression of the CRY1-1 transgene. Direct selection of emetine-resistant transformants was possible when transformed cells were first induced to differentiate into gametes by nitrogen starvation and then allowed to dedifferentiate back to vegetative cells before emetine selection was applied. With this transformation protocol, the RBCS2/CRY1-1 dominant selectable marker gene is a powerful tool for many molecular genetic applications in C. reinhardtii.

Alteration of flower color in Iris germanica L. ‘Fire Bride’ through ectopic expression of phytoene synthase gene (crtB) from Pantoea agglomerans

2014 ◽  
Vol 33 (8) ◽  
pp. 1307-1321 ◽  
Author(s):  
Zoran Jeknić ◽  
Stevan Jeknić ◽  
Slađana Jevremović ◽  
Angelina Subotić ◽  
Tony H. H. Chen
Keyword(s):  
Ectopic Expression ◽  
Flower Color ◽  
Pantoea Agglomerans ◽  
Phytoene Synthase ◽  
Iris Germanica ◽  
Phytoene Synthase Gene

scholarly journals The phytoene synthase gene family in the Grasses

2009 ◽  
Vol 4 (3) ◽  
pp. 208-211 ◽  
Author(s):  
Faqiang Li ◽  
Oren Tzfadia ◽  
Eleanore T. Wurtzel
Keyword(s):  
Gene Family ◽  
Phytoene Synthase ◽  
Phytoene Synthase Gene

Stable nuclear transformation of Chlamydomonas reinhardtii with a Streptomyces rimosus gene as the selective marker

Gene ◽  
1996 ◽  
Vol 181 (1-2) ◽  
pp. 13-18 ◽  
Author(s):  
Irina A. Sizova ◽  
Tatyana V. Lapina ◽  
Olga N. Frolova ◽  
Nelly N. Alexandrova ◽  
Konstantin E. Akopiants ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Nuclear Transformation ◽  
Streptomyces Rimosus ◽  
Selective Marker

scholarly journals White Mutants of Chlamydomonas reinhardtii Are Defective in Phytoene Synthase

Genetics ◽  
2004 ◽  
Vol 168 (3) ◽  
pp. 1249-1257 ◽  
Author(s):  
Sarah S. McCarthy ◽  
Marilyn C. Kobayashi ◽  
Krishna K. Niyogi
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Phytoene Synthase

Phytoene Accumulation in Sunflower Decreases the Transcript Levels of the Phytoene Synthase Gene

2006 ◽  
Vol 48 (1) ◽  
pp. 79-87 ◽  
Author(s):  
L. Campisi ◽  
M. Fambrini ◽  
V. Michelotti ◽  
M. Salvini ◽  
D. Giuntini ◽  
...  
Keyword(s):  
Phytoene Synthase ◽  
Transcript Levels ◽  
Phytoene Synthase Gene

Identification of QTL and association of a phytoene synthase gene with endosperm colour in durum wheat

2006 ◽  
Vol 114 (3) ◽  
pp. 525-537 ◽  
Author(s):  
C. J. Pozniak ◽  
R. E. Knox ◽  
F. R. Clarke ◽  
J. M. Clarke
Keyword(s):  
Durum Wheat ◽  
Phytoene Synthase ◽  
Phytoene Synthase Gene
Sign in / Sign up

Export Citation Format

Share Document