Regulation and function of the flavonoid-inducible efflux system, emrR-emrAB, in Agrobacterium tumefaciens C58

2019 ◽  
Vol 103 (14) ◽  
pp. 5763-5780 ◽  
Author(s):  
Sasimaporn Khemthong ◽  
Puttamas Nuonming ◽  
Thanittra Dokpikul ◽  
Rojana Sukchawalit ◽  
Skorn Mongkolsuk
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Efflux System ◽  
Agrobacterium Tumefaciens C58 ◽  
And Function

NieR is the repressor of a NaOCl-inducible efflux system in Agrobacterium tumefaciens C58

2021 ◽  
pp. 126816
Author(s):  
Jintana Duang-Nkern ◽  
Benya Nontaleerak ◽  
Tham Udomkanarat ◽  
Kritsakorn Saninjuk ◽  
Rojana Sukchawalit ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Efflux System ◽  
Agrobacterium Tumefaciens C58

scholarly journals Characterization and regulation of AcrABR, a RND-type multidrug efflux system, in Agrobacterium tumefaciens C58

2018 ◽  
Vol 214 ◽  
pp. 146-155 ◽  
Author(s):  
Puttamas Nuonming ◽  
Sasimaporn Khemthong ◽  
Thanittra Dokpikul ◽  
Rojana Sukchawalit ◽  
Skorn Mongkolsuk
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Multidrug Efflux ◽  
Efflux System ◽  
Agrobacterium Tumefaciens C58 ◽  
Multidrug Efflux System

Characterization of a putative RND-type efflux system in Agrobacterium tumefaciens

Gene ◽  
2001 ◽  
Vol 270 (1-2) ◽  
pp. 245-252 ◽  
Author(s):  
Wen-Tao Peng ◽  
Eugene W Nester
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Efflux System

scholarly journals Two Agrobacterium tumefaciens CheW Proteins Are Incorporated into One Chemosensory Pathway with Different Efficiencies

2018 ◽  
Vol 31 (4) ◽  
pp. 460-470 ◽  
Author(s):  
Zhiwei Huang ◽  
Qingxuan Zhou ◽  
Pan Sun ◽  
Jing Yang ◽  
Minliang Guo
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Histidine Kinase ◽  
Promoter Activity ◽  
Crown Gall Tumor ◽  
Signaling Complex ◽  
Chemosensory Pathway ◽  
Dicotyledonous Plants ◽  
The Difference ◽  
Coupling Protein ◽  
And Function

Agrobacterium tumefaciens is the agent that causes crown gall tumor disease on more than 140 species of dicotyledonous plants. Chemotaxis of A. tumefaciens toward the wound sites of the host plant is the first step to recognize the host. CheW is a coupling protein that bridges the histidine kinase CheA and the chemoreceptors to form the chemotaxis core signaling complex and plays a crucial role in the assembly and function of the large chemosensory array. Unlike all previously reported chemotaxis systems, A. tumefaciens has only one major che operon but two cheW homologs (atu2075 as cheW1 and atu2617 as cheW2) on unlinked loci. The in-frame deletion of either cheW gene significantly affects A. tumefaciens chemotaxis but does not abolish the chemotaxis, unless both cheW genes were deleted. The effect of cheW2 deletion on the chemotaxis is more severe than that of cheW1 deletion. Either CheW can interact with CheA and couple it to the cell poles. The promoter activity of cheW2 is always higher than that of cheW1 under all of the tested conditions. When two cheW genes were adjusted to the same expression level by using the identical promoter, the difference between the effects of two CheW proteins on the chemotaxis still existed. Therefore, we envision that both the different molecular ratio of two CheW proteins in cell and the different affinities of two CheW proteins with CheA and chemoreceptors result in the efficiency difference of two CheW proteins in functioning in the large chemosensory array.

scholarly journals Different Regulation and Roles of Lactonases AiiB and AttM in Agrobacterium tumefaciens C58

2009 ◽  
Vol 22 (5) ◽  
pp. 529-537 ◽  
Author(s):  
Elise Haudecoeur ◽  
Mélanie Tannières ◽  
Amélie Cirou ◽  
Aurélie Raffoux ◽  
Yves Dessaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Selective Advantage ◽  
In Planta ◽  
Host Interaction ◽  
Plant Tumors ◽  
Plant Signals ◽  
Polymerase Chain ◽  
Agrobacterium Tumefaciens C58 ◽  
Regulated Functions

The phytopathogen Agrobacterium tumefaciens C58 expresses two lactonases, AttM and AiiB. We showed that expression of the aiiB gene was controlled by agrocinopines A and B and required the agrocinopine-ABC transporter Acc, but was not affected by the level of quorum-sensing (QS) signal 3-oxo-octanoylhomoserine lactone (OC8-HSL). In the presence of agrocinopines, a constructed aiiB mutant accumulated OC8-HSL at a level 10-fold higher than that of the wild-type strain, and showed an exacerbated expression of a key QS-regulated function, conjugation of Ti plasmid (in vitro and in planta), as well as an increase of the number of emerging tumors on the host plant. The expression and acyl-HSL-degrading activity of AttM were evident in the presence of wounded tissues; however, in unwounded plant tumors, the QS-regulated functions were weakly affected in an attM mutant. By contrast, we observed that attM conferred a selective advantage in the course of colonization of plant tumors. Finally, polymerase chain reaction survey of genes attM and aiiB showed that they were not strictly conserved in the genus Agrobacterium. This work proved that the lactonases AttM and AiiB are regulated by different plant signals and are implicated in different functions in the course of the A. tumefaciens C58–host interaction.

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