Analysis of predictors influencing indeterminate whole-blood interferon-gamma release assay results in patients with rheumatic diseases

2014 ◽  
Vol 34 (12) ◽  
pp. 1711-1720 ◽  
Author(s):  
Hyun-Ju Jung ◽  
Tae-Jong Kim ◽  
Hyoung-Sang Kim ◽  
Young-Nan Cho ◽  
Hye-Mi Jin ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Rheumatic Diseases ◽  
Whole Blood ◽  
Interferon Gamma Release Assay ◽  
Release Assay

scholarly journals Whole-Blood Interferon-Gamma Release Assay for Diagnosis of Tuberculous Lymphadenitis

2011 ◽  
Vol 224 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Young Keun Kim ◽  
Young Uh ◽  
Nam Seok Lee ◽  
Mee Yon Cho ◽  
Minseob Eom ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Whole Blood ◽  
Interferon Gamma Release Assay ◽  
Tuberculous Lymphadenitis ◽  
Release Assay

Usefulness of the whole-blood interferon-gamma release assay for diagnosis of extrapulmonary tuberculosis

2009 ◽  
Vol 63 (2) ◽  
pp. 182-187 ◽  
Author(s):  
Kyoung-Ho Song ◽  
Jae Hyun Jeon ◽  
Wan Beom Park ◽  
Sung-Han Kim ◽  
Kyoung Un Park ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Whole Blood ◽  
Extrapulmonary Tuberculosis ◽  
Interferon Gamma Release Assay ◽  
Release Assay

scholarly journals A Bayesian analysis of a Test and Vaccinate or Remove study to control bovine tuberculosis in badgers (Meles meles)

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0246141
Author(s):  
Mark E. Arnold ◽  
Emily A. Courcier ◽  
Lesley A. Stringer ◽  
Carl M. McCormick ◽  
Ana V. Pascual-Linaza ◽  
...  
Keyword(s):  
Real Time ◽  
Diagnostic Test ◽  
Interferon Gamma ◽  
Whole Blood ◽  
Latent Class ◽  
Meles Meles ◽  
Interferon Gamma Release Assay ◽  
Infection Prevalence ◽  
Test Characteristics ◽  
Release Assay

A novel five year Test and Vaccinate or Remove (TVR) wildlife research intervention project in badgers (Meles meles) commenced in 2014 in a 100km2 area of Northern Ireland. It aimed to increase the evidence base around badgers and bovine TB and help create well-informed and evidence-based strategies to address the issue of cattle-to-cattle spread and spread between cattle and badgers. It involved real-time trap-side testing of captured badgers and vaccinating those that tested negative for bTB (BadgerBCG–BCG Danish 1331) and removal of those that tested bTB positive using the Dual-Path Platform VetTB test (DPP) for cervids (Chembio Diagnostic Systems, Medford, NY USA). Four diagnostic tests were utilised within the study interferon gamma release assay (IGRA), culture (clinical samples and post mortem), DPP using both whole blood and DPP using serum. BCG Sofia (SL222) was used in the final two years because of supply issues with BadgerBCG. Objectives for this study were to evaluate the performance of the DPP in field conditions and whether any trend was apparent in infection prevalence over the study period. A Bayesian latent class model of diagnostic test evaluation in the absence of a gold standard was applied to the data. Temporal variation in the sensitivity of DPP and interferon gamma release assay (IGRA) due to the impact of control measures was investigated using logistic regression and individual variability was assessed. Bayesian latent class analysis estimated DPP with serum to have a sensitivity of 0.58 (95% CrI: 0.40–0.76) and specificity of 0.97 (95% CrI: 0.95–0.98). The DPP with whole blood showed a higher sensitivity (0.69 (95% CrI: 0.48–0.88)) but similar specificity (0.98 (95% Crl: 0.96–0.99)). The change from BCG Danish to BCG Sofia significantly impacted on DPP serum test characteristics. In addition, there was weak evidence of increasing sensitivity of IGRA over time and differences in DPP test sensitivity between adults and cubs. An exponential decline model was an appropriate representation of the infection prevalence over the 5 years, with a starting prevalence of 14% (95% CrI: 0.10–0.20), and an annual reduction of 39.1% (95% CrI: 26.5–50.9). The resulting estimate of infection prevalence in year 5 of the study was 1.9% (95% CrI: 0.8–3.8). These results provide field evidence of a statistically significant reduction in badger TB prevalence supporting a TVR approach to badger intervention. They give confidence in the reliability and reproducibility in the DPP Whole Blood as a real time trap-side diagnostic test for badgers, and describe the effect of vaccination and reduced infection prevalence on test characteristics.

The Effect of Age on Whole Blood Interferon-Gamma Release Assay Response among Children Investigated for Latent Tuberculosis Infection

2012 ◽  
Vol 161 (4) ◽  
pp. 632-638 ◽  
Author(s):  
Elena Critselis ◽  
Virginia Amanatidou ◽  
Garyfallia Syridou ◽  
Nikos P. Spyridis ◽  
Mersini Mavrikou ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Whole Blood ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Interferon Gamma Release Assay ◽  
Release Assay ◽  
Effect Of Age

scholarly journals Diagnosis and Risk Factors of Latent Tuberculosis Infection among Healthcare Workers Using Whole Blood Human Interferon-gamma Release Assay and Tuberculin Skin Testing

2020 ◽  
pp. 15-21
Author(s):  
A. N. Umo ◽  
O. J. Akinjogunla ◽  
N. O. Umoh ◽  
G. E. Uzono
Keyword(s):  
Risk Factors ◽  
Interferon Gamma ◽  
Whole Blood ◽  
Healthcare Workers ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Interferon Gamma Release Assay ◽  
Tuberculin Skin Testing ◽  
Release Assay

This study established the diagnosis and risk factors of latent tuberculosis infection (LTBI) among health-care workers in an endemic population using Tuberculin skin test (TST) and Quantiferon TB-gold. A total of 609 Healthcare workers from tuberculosis treatment facilities in Akwa Ibom State, Nigeria were studied. The Interferon-gamma release assay was performed using 3ml of whole blood by ELISA according to the manufacturer’s instruction (Cellestis Ltd., Carnegie, Australia) after which 0.1 ml of 5 tuberculin units of Purified Protein Derivative (PPD) was administered intra-dermally to each subject. TST results were read after 72 hours by measuring the size of indurations in millimetres. Data were analysed using SPSS version 17 (SPSS Inc., Chicago, Illinois). At the threshold of 10 mm, the prevalence of LTBI by TST was 45.8% and 24.8% at the IGRA diagnostic value of ≥ 0.351 IU. Laboratory staff and ward orderlies as well as being in service for >10 years, were more significantly associated with LTBI. A moderate agreement of 76.7%, k = 0.51 was obtained between TST at 10 mm, and QFT. Neither previous exposure to TST nor BCG vaccination affected the prevalence of LTBI in the study population. The difference of 54% prevalence of LTBI between TST and QFT may be due to non-tuberculous mycobacterium (NTM) since TST is non-specific. This may have grave implications of drug toxicity and development of resistance to anti-TB drug among individuals harbouring NTM, but receiving anti-TB medication. The 76.7% agreement between the two tests is an indication that the 10 mm cut-off induration for TST is still relevant in the diagnosis of LTBI.

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