Heritable transgene expression pattern imposed onto maize ubiquitin promoter by maize adh-1 matrix attachment regions: tissue and developmental specificity in maize transgenic plants

2004 ◽  
Vol 22 (12) ◽  
Author(s):  
Fran�ois Torney ◽  
Anne Partier ◽  
V�ronique Says-Lesage ◽  
Isabelle Nadaud ◽  
Pierre Barret ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Expression Pattern ◽  
Transgene Expression ◽  
Matrix Attachment Regions ◽  
Ubiquitin Promoter ◽  
Maize Ubiquitin Promoter

scholarly journals Effect of nuclear matrix attachment regions on transgene expression in tobacco plants.

2001 ◽  
Vol 48 (3) ◽  
pp. 637-646 ◽  
Author(s):  
W Nowak ◽  
M Gawłowska ◽  
A Jarmołowski ◽  
J Augustyniak
Keyword(s):  
Transgenic Plants ◽  
Reporter Gene ◽  
Transgene Expression ◽  
Binary Vector ◽  
Special Property ◽  
Matrix Attachment Regions ◽  
Plant Populations ◽  
Tobacco Plants ◽  
Gus Reporter ◽  
Loop Domains

Matrix attachment regions (MARs) are thought to participate in the organization and segregation of independent chromosomal loop domains. Although there are several reports on the action of natural MARs in the context of heterologous genes in transgenic plants, in our study we tested a synthetic MAR (sMAR) with the special property of unpairing when under superhelical strain, for its effect on reporter gene expression in tobacco plants. The synthetic MAR was a multimer of a short sequence from the MAR 3' end of the immunoglobulin heavy chain (IgH) enhancer. This sMAR sequence was used to flank the beta-glucuronidase (GUS) reporter gene within the T-DNA of the binary vector pBI121. Vectors with or without the sMARs were then used to transform tobacco plants by Agrobacterium tumefaciens. Transgenic plants containing the sMAR sequences flanking the GUS gene exhibited higher levels of transgene expression compared with transgenic plants which lacked the sMARs. This effect was observed independently of the position of the sMAR at the 5' side of the reporter gene. However, variation of the detected transgene expression was significant in all transformed plant populations, irrespective of the construct used.

Matrix attachment regions and regulated transcription increase and stabilize transgene expression

2005 ◽  
Vol 3 (5) ◽  
pp. 535-543 ◽  
Author(s):  
Rita Abranches ◽  
Randall W. Shultz ◽  
William F. Thompson ◽  
George C. Allen
Keyword(s):  
Transgene Expression ◽  
Matrix Attachment Regions

Over-expression of the rice OsAMT1-1 gene increases ammonium uptake and content, but impairs growth and development of plants under high ammonium nutrition

2006 ◽  
Vol 33 (2) ◽  
pp. 153 ◽  
Author(s):  
Mohammad S. Hoque ◽  
Josette Masle ◽  
Michael K. Udvardi ◽  
Peter R. Ryan ◽  
Narayana M. Upadhyaya
Keyword(s):  
Transgenic Plants ◽  
Ammonium Assimilation ◽  
Ammonium Transporter ◽  
Ammonium Uptake ◽  
Vegetative Stage ◽  
Over Expression ◽  
Transgenic Lines ◽  
Ammonium Nutrition ◽  
Maize Ubiquitin Promoter

A transgenic approach was undertaken to investigate the role of a rice ammonium transporter (OsAMT1-1) in ammonium uptake and consequent ammonium assimilation under different nitrogen regimes. Transgenic lines overexpressing OsAMT1-1 were produced by Agrobacterium-mediated transformation of two rice cultivars, Taipei 309 and Jarrah, with an OsAMT1-1 cDNA gene construct driven by the maize ubiquitin promoter. Transcript levels of OsAMT1-1 in both Taipei 309 and Jarrah transgenic lines correlated positively with transgene copy number. Shoot and root biomass of some transgenic lines decreased during seedling and early vegetative stage compared to the wild type, especially when grown under high (2 mm) ammonium nutrition. Transgenic plants, particularly those of cv. Jarrah recovered in the mid-vegetative stage under high ammonium nutrition. Roots of the transgenic plants showed increased ammonium uptake and ammonium content. We conclude that the decreased biomass of the transgenic lines at early stages of growth might be caused by the accumulation of ammonium in the roots owing to the inability of ammonium assimilation to match the greater ammonium uptake.

scholarly journals Matrix attachment regions increase transgene expression levels and stability in transgenic rice plants and their progeny

1999 ◽  
Vol 18 (3) ◽  
pp. 233-242 ◽  
Author(s):  
Philippe Vain ◽  
Barbara Worland ◽  
Ajay Kohli ◽  
John W. Snape ◽  
Paul Christou ◽  
...  
Keyword(s):  
Transgenic Rice ◽  
Transgene Expression ◽  
Matrix Attachment Regions ◽  
Expression Levels ◽  
Rice Plants ◽  
Transgenic Rice Plants

From transgene expression to public acceptance of transgenic plants: a matter of predictability

1996 ◽  
Vol 45 (1-3) ◽  
pp. 5-10 ◽  
Author(s):  
Jan-Peter Nap ◽  
Ľudmila Mlynárová ◽  
Willem J. Stiekema
Keyword(s):  
Transgenic Plants ◽  
Transgene Expression ◽  
Public Acceptance
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