Histological changes associated with acquisition of competence for shoot regeneration in leaf discs of Saintpaulia ionantha ×confusa hybrid (African violet) cultured in vitro

1997 ◽  
Vol 16 (6) ◽  
pp. 421-425
Author(s):  
K. H. Lo ◽  
K. L. Giles ◽  
V. K. Sawhney
Keyword(s):  
Shoot Regeneration ◽  
Histological Changes ◽  
Leaf Discs ◽  
African Violet ◽  
Saintpaulia Ionantha

scholarly journals In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

2007 ◽  
Vol 50 (2) ◽  
pp. 239-247 ◽  
Author(s):  
Simone Pacheco Lombardi ◽  
Ilene Ribeiro da Silva Passos ◽  
Maria Cristina Stolf Nogueira ◽  
Beatriz Appezzato-da-Glória
Keyword(s):  
Secondary Structure ◽  
Shoot Regeneration ◽  
Callus Formation ◽  
Primary Root ◽  
Bud Formation ◽  
Leaf Discs ◽  
Root Segments ◽  
Seedling Roots ◽  
In Vitro Shoot Regeneration

Different concentrations of 6-benzyladenine (6-BA) were used in vitro to induce buds in three types of explants: leaf discs, root segments and the seedling itself obtained from in vitro seed germination of Passiflora cincinnata Mast. The 0.5 mg.L -1 6-BA concentration was most suitable for all the three explants, however, bud formation time and means (direct/indirect) were different for each type of explant. In seedlings used as explants, it had shoot regeneration from the primary root. Histological events leading to bud formation via meristemoids were described. The origin was indirect in leaf discs, with callus formation in subepidermal cells of the chlorophyll parenchyma layers. In root segments and in seedling roots, the buds were formed directly in the pericycle (in roots with some secondary structure) and in the vascular cambium (at the initial phase of secondary structure). Also, indirect buds originated from meristemoids which were formed around the callus.

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