In situ hybridization and immuno-electron microscope analyses of the Us11 gene of herpes simplex virus type 1 during transient expression

Chromosoma ◽  
1996 ◽  
Vol 104 (6) ◽  
pp. 434-444
Author(s):  
Sylvie Besse ◽  
Jean-Jacques Diaz ◽  
Evelyne Pichard ◽  
Karine Kindbeiter ◽  
Jean-Jacques Madjar ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscope ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Transient Expression ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Simplex Virus

In situ hybridization and immuno-electron microscope analyses of the Us11 gene of herpes simplex virus type 1 during transient expression

Chromosoma ◽  
1996 ◽  
Vol 104 (6) ◽  
pp. 434-444 ◽  
Author(s):  
Sylvie Besse ◽  
Jean-Jacques Diaz ◽  
Evelyne Pichard ◽  
Karine Kindbeiter ◽  
Jean-Jacques Madjar ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscope ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Transient Expression ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Simplex Virus

Detection of Herpes Simplex Virus Type-1 DNA by In Situ Polymerase Chain Reaction

1995 ◽  
Vol 7 (3) ◽  
pp. 211
Author(s):  
Jee-Ho Choi ◽  
Seong-Beom Kim ◽  
Jung-Wha Suh ◽  
In-Sung Song ◽  
Jai-Kyoung Koh ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Simplex Virus

scholarly journals Famciclovir and Valaciclovir Differ in the Prevention of Herpes Simplex Virus Type 1 Latency in Mice: a Quantitative Study

1998 ◽  
Vol 42 (7) ◽  
pp. 1555-1562 ◽  
Author(s):  
Alana M. Thackray ◽  
Hugh J. Field
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Culture Method ◽  
Explant Culture ◽  
Latently Infected ◽  
Simplex Virus

ABSTRACT Famciclovir (FCV) and valaciclovir (VACV) have previously been shown to be potent inhibitors of herpes simplex virus type 1 (HSV-1) in a murine cutaneous model. In the present study, mice were inoculated in the skin of the left ear pinna with herpes simplex virus (HSV) type 1. Antiviral therapy was started on different days postinoculation (p.i.), terminating at the end of day 10 p.i. The compounds were administered twice daily by oral gavage at 50 mg/kg of body weight/dose. Mice were sampled on day 5 p.i., during the acute phase of the infection, and the titers of infectious virus in the target tissues (ear, brain stem, and trigeminal ganglia) were determined. At 2 to 3 months p.i., the ipsilateral and contralateral trigeminal and cervical dorsal root ganglia were explanted, and four different methods were used to detect latent HSV. The methods were (i) conventional explant culture for 5 days followed by homogenization, (ii) long-term culture (up to 73 days) of whole ganglia, followed by homogenization, (iii) dissociation by enzymatic disaggregation and an infectious center assay, and (iv) in situ hybridization to detect latency-associated transcripts (LATs). The conventional explant culture method was the least sensitive method, while in situ staining for LAT was the most sensitive, and all mice, including those treated from early times with FCV, were shown to be latently infected. Significantly less latent virus was detected by all four methods, however, in ganglia obtained from mice that had been treated with FCV in comparison with the amount detected in ganglia from mice that had been treated with VACV. However, in no case was latency completely eliminated.

Valacyclovir inhibition of recovery of ocular herpes simplex virus type 1 after experimental reactivation by laser in situ keratomileusis

2001 ◽  
Vol 27 (8) ◽  
pp. 1288-1293 ◽  
Author(s):  
Deepinder K Dhaliwal ◽  
Eric G Romanowski ◽  
Kathleen A Yates ◽  
Dean Hu ◽  
Francis S Mah ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Laser In Situ Keratomileusis ◽  
Ocular Herpes ◽  
Simplex Virus
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