Induction of complete segregation of cellular DNA and non-encapsidated viral genomes in herpes simplex virus type 1 infected HeLa cells as revealed by in situ hybridization

Chromosoma ◽  
1994 ◽  
Vol 103 (2) ◽  
pp. 104-110
Author(s):  
Francine Puvion-Dutilleul ◽  
Sylvie Besse
Keyword(s):  
In Situ Hybridization ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Hela Cells ◽  
Herpes Simplex Virus Type ◽  
Viral Genomes ◽  
Cellular Dna ◽  
Simplex Virus

In situ hybridization and immuno-electron microscope analyses of the Us11 gene of herpes simplex virus type 1 during transient expression

Chromosoma ◽  
1996 ◽  
Vol 104 (6) ◽  
pp. 434-444 ◽  
Author(s):  
Sylvie Besse ◽  
Jean-Jacques Diaz ◽  
Evelyne Pichard ◽  
Karine Kindbeiter ◽  
Jean-Jacques Madjar ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscope ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Transient Expression ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Simplex Virus

In situ hybridization and immuno-electron microscope analyses of the Us11 gene of herpes simplex virus type 1 during transient expression

Chromosoma ◽  
1996 ◽  
Vol 104 (6) ◽  
pp. 434-444
Author(s):  
Sylvie Besse ◽  
Jean-Jacques Diaz ◽  
Evelyne Pichard ◽  
Karine Kindbeiter ◽  
Jean-Jacques Madjar ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscope ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Transient Expression ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Simplex Virus

Quiescent viral genomes in human fibroblasts after infection with herpes simplex virus type 1 Vmw65 mutants

1995 ◽  
Vol 76 (6) ◽  
pp. 1417-1431 ◽  
Author(s):  
D. R. S. Jamieson ◽  
L. H. Robinson ◽  
J. I. Daksis ◽  
M. J. Nicholl ◽  
C. M. Preston
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Human Fibroblasts ◽  
Viral Genomes ◽  
Simplex Virus

Detection of Herpes Simplex Virus Type-1 DNA by In Situ Polymerase Chain Reaction

1995 ◽  
Vol 7 (3) ◽  
pp. 211
Author(s):  
Jee-Ho Choi ◽  
Seong-Beom Kim ◽  
Jung-Wha Suh ◽  
In-Sung Song ◽  
Jai-Kyoung Koh ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Simplex Virus

scholarly journals Herpes Simplex Virus Type 1 Genomes Are Associated with ND10 Nuclear Substructures in Quiescently Infected Human Fibroblasts

2007 ◽  
Vol 81 (20) ◽  
pp. 10991-11004 ◽  
Author(s):  
Roger D. Everett ◽  
Jill Murray ◽  
Anne Orr ◽  
Chris M. Preston
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Human Fibroblasts ◽  
Lytic Infection ◽  
Viral Genomes ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT Herpes simplex virus type 1 (HSV-1) genomes become associated with structures related to cellular nuclear substructures known as ND10 or promyelocytic leukemia nuclear bodies during the early stages of lytic infection. This paper describes the relationship between HSV-1 genomes and ND10 in human fibroblasts that maintain the viral genomes in a quiescent state. We report that quiescent HSV-1 genomes detected by fluorescence in situ hybridization (FISH) are associated with enlarged ND10-like structures, frequently such that the FISH-defined viral foci are apparently enveloped within a sphere of PML and other ND10 proteins. The number of FISH viral foci in each quiescently infected cell is concordant with the input multiplicity of infection, with each structure containing no more than a small number of viral genomes. A proportion of the enlarged ND10-like foci in quiescently infected cells contain accumulations of the heterochromatin protein HP1 but not other common markers of heterochromatin such as histone H3 di- or trimethylated on lysine residue 9. Many of the virally induced enlarged ND10-like structures also contain concentrations of conjugated ubiquitin. Quiescent infections can be established in cells that are highly depleted for PML. However, during the initial stages of establishment of a quiescent infection in such cells, other ND10 proteins (Sp100, hDaxx, and ATRX) are recruited into virally induced foci that are likely to be associated with HSV-1 genomes. These observations illustrate that the intimate connections between HSV-1 genomes and ND10 that occur during lytic infection also extend to quiescent infections.

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