Diversity of nuclear microsatellite markers were analyzed in a reference
sample of the population of northeast Bosnia and Herzegovina. 437 samples
taken from unrelated individuals were processed and three samples of
paternity proof were shown. Detection effectiveness profile of the research,
points to a valid choice of method of extraction, amplification and
genotyping short tandem repeat (STR) loci with PowerPlextm16 kit. Genetic
analysis of allelic variants of the 15 STR loci PowerPlextm16 kit detected 17
samples determined as rare allelic variants or microvariants. Samples were
divided into 15 different allelic variants at 7 different loci, and are: in
locus D7S820, D16S539, D3S1358, D18S51, PENTA D, PENTA E and in locus vWA.
Genetic analysis of mutations in cases of paternity determined three examples
of single-step mutations in the loci FGA, Penta D and D3S1358. Genetic
analysis of observed STR loci detected three allelic variant of genotype
combination 7/10/11.3 in locus D7S820 Type II. Population genetic analysis of
STR loci in a representative sample of the population of northeast Bosnia and
Herzegovina included the application of the assessment tests of
within-population genetic diversity and interpopulation diversity, as well as
genetic differentiation between populations: North-eastern Bosnia and
Herzegovina (BH) and BH general reference, then the Croatian population,
Macedonian, Serbian and Slovenian. Based on the result analysis of specific
forensic parameters, it can be assumed that the most informative marker is
PENTA E for population genetic analysis and forensic testing in the
population of northeast Bosnia and Herzegovina. Research results fit regional
STR database of this part of Europe.
Forensic and population genetic analysis of Serbian population using 21 STR loci of GlobalFiler™ PCR amplification kit
