Three-dimensional reconstruction by confocal laser scanning microscopy in routine pathologic specimens of benign and malignant lesions of the human breast

1997 ◽  
Vol 107 (4) ◽  
pp. 267-278 ◽  
Author(s):  
S. Liu ◽  
Donald L. Weaver ◽  
D. J. Taatjes
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Three Dimensional Reconstruction ◽  
Human Breast ◽  
Dimensional Reconstruction ◽  
Malignant Lesions

Application of Phase Correlation to the Montage Synthesis and Three-Dimensional Reconstruction of Large Tissue Volumes from Confocal Laser Scanning Microscopy

2006 ◽  
Vol 12 (2) ◽  
pp. 106-112 ◽  
Author(s):  
Mohamed-Adel Slamani ◽  
Andrzej Krol ◽  
Jacques Beaumont ◽  
Robert L. Price ◽  
Ioana L. Coman ◽  
...  
Keyword(s):  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Phase Correlation ◽  
Confocal Laser Scanning ◽  
Scanning Microscopy ◽  
Confocal Laser ◽  
Dimensional Reconstruction ◽  
3D Volume

We have implemented and tested a new automatic method for the montage synthesis and three-dimensional (3D) reconstruction of large tissue volumes from confocal laser scanning microscopy data (CLSM). This method relies on maximization of the phase correlation between adjacent images. It was tested on a large specimen (a murine heart) that was cut into a number of individual sections with thickness appropriate for CLSM. The sections were scanned horizontally (in-plane) and vertically (perpendicular to the optical planes) to produce “tiles” of a 3D volume. Phase correlation maximization was applied to the montage synthesis of in-plane tiles and 3D alignment of optical slices within a given physical section. The performance of the new method is evaluated.

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